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Reviewed, UniProtKB/Swiss-Prot Q4J9S8 (PCKG_SULAC)

Last modified June 16, 2009. Version 28. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Phosphoenolpyruvate carboxykinase [GTP]
      Short name=PEP carboxykinase
      Short name=PEPCK
    EC=4.1.1.32
Alternative name(s):
    Phosphoenolpyruvate carboxylase
Gene names
Name: pckG
Ordered Locus Names: Saci_1100
OrganismSulfolobus acidocaldarius [Complete proteome] [HAMAP]
Taxonomic identifier2285 [NCBI]
Taxonomic lineageArchaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

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Protein attributes

Sequence length604 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity.

Catalytic activity

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2. HAMAP MF_00452

Cofactor

Binds 1 manganese ion per subunit By similarity.

Pathway

Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00452

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

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Ontologies

Keywords
   Biological processGluconeogenesis
   Cellular componentCytoplasm
   LigandGTP-binding
Manganese
Metal-binding
Nucleotide-binding
   Molecular functionDecarboxylase
Lyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processgluconeogenesis

Inferred from electronic annotation. Source: HAMAP

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionGTP binding

Inferred from electronic annotation. Source: HAMAP

manganese ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoenolpyruvate carboxykinase (GTP) activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 604604Phosphoenolpyruvate carboxykinase [GTP] HAMAP MF_00452
PRO_0000103621

Regions

Region365 – 3673Substrate binding By similarity

Sites

Active site2601 By similarity
Metal binding2181Manganese By similarity
Metal binding2371Manganese By similarity
Metal binding2751Manganese By similarity
Binding site871Substrate By similarity
Binding site2111Substrate; via amide nitrogen By similarity
Binding site2181Substrate By similarity
Binding site2581Substrate By similarity
Binding site3671GTP By similarity
Binding site3981GTP By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q4J9S8-1 [UniParc].

Last modified August 2, 2005. Version 1.
Checksum: FFEA81938BF49096

FASTA60468,720
        10         20         30         40         50         60 
MKVDLSPLNG IISDKAFQKL KSINNPSLVH FLSKTIELTT PDRVYVSFGE EKDREYVKKR 

        70         80         90        100        110        120 
ALETKEEIKL KMEGHTIHFD HPLDQARARE DTFILTDEKI PFVNTKPRDE GLREMLSLLK 

       130        140        150        160        170        180 
GSMKGREMYV GFYSLGPRNS KFSILAVQIT DSPYVIHSEN ILYRNAFEDF YGDKPFLKFI 

       190        200        210        220        230        240 
HSKGQLDIKK RRIMIDVKEN TVYSVNTTYA GNSVGLKKLA LRLTVTKAVN EGWLSEHMAI 

       250        260        270        280        290        300 
VGFEGNRGTH YFTASFPSGS GKTSTSMLGS LISDDLAFIK EIDGVCRAVN PEIGIFGIIQ 

       310        320        330        340        350        360 
GINERDDPVI WDVLHKPGEV IFSNVLMTDD GGVYWEGSEV EKPEKGYNYE GAWTKESGKP 

       370        380        390        400        410        420 
ASHPNARFTS PLTSFSNLDK DYDNPQGVVI DGIIFGVRDY STLVPVTEAF SWEHGVITIG 

       430        440        450        460        470        480 
ASMESSRTSA VIGKADVLEF NPMAILDFMP LSLGKYLNNY LTFGRNLKYV PKIFSFNYFL 

       490        500        510        520        530        540 
KDENNKFLNS KEDKRVWVKW AVKRVESETD AIYTPVGFIP YYEDLSKLFN STLGKQYTKE 

       550        560        570        580        590        600 
AYELQFTLKL SKYLEKTERI MKIYSEIADT PIEVINELKA QKDRLLDYIN RYGDKVSPFQ 


LVKS

« Hide

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References

[1]"The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota."
Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E., Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A.
J. Bacteriol. 187:4992-4999(2005) [PubMed: 15995215] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33909 / DSM 639 / IFO 15157 / JCM 8929 / NCIB 11770.

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Cross-references

Sequence databases

CP000077 Genomic DNA. Translation: AAY80452.1.
RefSeqYP_255745.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID3474134.
GenomeReviewsGene locus Saci_1100 in contig CP000077_GR.
KEGGsai:Saci_1100.
NMPDRfig|330779.3.peg.1575.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ4J9S8.
OMAQ4J9S8. SHPNARF.

Enzyme and pathway databases

BioCycSACI330779:SACI_1100-MON.
BRENDA4.1.1.32. 434.

Family and domain databases

HAMAPMF_00452.
[Tree]
InterProIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
Gene3DG3DSA:3.90.228.20. PEP_carboxykinase_C. 1 hit.
G3DSA:3.40.449.10. PEP_carboxykinase_N. 1 hit.
PANTHERPTHR11561. PEP_carboxykin. 1 hit.
PfamPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
ProDomPD004738. PEPCK_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEPS00505. PEPCK_GTP. False negative.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry namePCKG_SULAC
AccessionPrimary (citable) accession number: Q4J9S8
Entry history
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: August 2, 2005
Last modified: June 16, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents