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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei87SubstrateUniRule annotation1
Metal bindingi218ManganeseUniRule annotation1
Metal bindingi237Manganese; via tele nitrogenUniRule annotation1
Binding sitei258SubstrateUniRule annotation1
Active sitei260UniRule annotation1
Metal bindingi275ManganeseUniRule annotation1
Binding sitei367GTPUniRule annotation1
Binding sitei398GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi259 – 264GTPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:Saci_1100
OrganismiSulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Taxonomic identifieri330779 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001018 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001036211 – 604Phosphoenolpyruvate carboxykinase [GTP]Add BLAST604

Interactioni

Protein-protein interaction databases

STRINGi330779.Saci_1100.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni209 – 211Substrate bindingUniRule annotation3
Regioni365 – 367Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG05865. Archaea.
COG1274. LUCA.
HOGENOMiHOG000191700.
KOiK01596.
OMAiEFNPFAI.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiIPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.

Sequencei

Sequence statusi: Complete.

Q4J9S8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVDLSPLNG IISDKAFQKL KSINNPSLVH FLSKTIELTT PDRVYVSFGE
60 70 80 90 100
EKDREYVKKR ALETKEEIKL KMEGHTIHFD HPLDQARARE DTFILTDEKI
110 120 130 140 150
PFVNTKPRDE GLREMLSLLK GSMKGREMYV GFYSLGPRNS KFSILAVQIT
160 170 180 190 200
DSPYVIHSEN ILYRNAFEDF YGDKPFLKFI HSKGQLDIKK RRIMIDVKEN
210 220 230 240 250
TVYSVNTTYA GNSVGLKKLA LRLTVTKAVN EGWLSEHMAI VGFEGNRGTH
260 270 280 290 300
YFTASFPSGS GKTSTSMLGS LISDDLAFIK EIDGVCRAVN PEIGIFGIIQ
310 320 330 340 350
GINERDDPVI WDVLHKPGEV IFSNVLMTDD GGVYWEGSEV EKPEKGYNYE
360 370 380 390 400
GAWTKESGKP ASHPNARFTS PLTSFSNLDK DYDNPQGVVI DGIIFGVRDY
410 420 430 440 450
STLVPVTEAF SWEHGVITIG ASMESSRTSA VIGKADVLEF NPMAILDFMP
460 470 480 490 500
LSLGKYLNNY LTFGRNLKYV PKIFSFNYFL KDENNKFLNS KEDKRVWVKW
510 520 530 540 550
AVKRVESETD AIYTPVGFIP YYEDLSKLFN STLGKQYTKE AYELQFTLKL
560 570 580 590 600
SKYLEKTERI MKIYSEIADT PIEVINELKA QKDRLLDYIN RYGDKVSPFQ

LVKS
Length:604
Mass (Da):68,720
Last modified:August 2, 2005 - v1
Checksum:iFFEA81938BF49096
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000077 Genomic DNA. Translation: AAY80452.1.
RefSeqiWP_011277954.1. NC_007181.1.

Genome annotation databases

EnsemblBacteriaiAAY80452; AAY80452; Saci_1100.
GeneIDi3474134.
KEGGisai:Saci_1100.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000077 Genomic DNA. Translation: AAY80452.1.
RefSeqiWP_011277954.1. NC_007181.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi330779.Saci_1100.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAY80452; AAY80452; Saci_1100.
GeneIDi3474134.
KEGGisai:Saci_1100.

Phylogenomic databases

eggNOGiarCOG05865. Archaea.
COG1274. LUCA.
HOGENOMiHOG000191700.
KOiK01596.
OMAiEFNPFAI.

Enzyme and pathway databases

UniPathwayiUPA00138.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiIPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPCKG_SULAC
AccessioniPrimary (citable) accession number: Q4J9S8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: August 2, 2005
Last modified: November 2, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.