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Protein

Serine/threonine-protein kinase ArnS

Gene

arnS

Organism
Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an essential role in the controlled expression of archaellum components during starvation-induced motility. May inhibit arnR transcription and promote ArnR translation.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Enzyme regulationi

Autophosphorylation is stimulated by Mn2+.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei344ATPPROSITE-ProRule annotation1
Active sitei460Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi323 – 331ATPPROSITE-ProRule annotation9

GO - Molecular functioni

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processStress response
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSACI330779:GH9J-1146-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase ArnS1 Publication (EC:2.7.11.11 Publication)
Short name:
Ser/Thr protein kinase ArnS1 Publication
Alternative name(s):
Archaellum regulatory network protein ArnSCurated
Gene namesi
Name:arnS1 Publication
Ordered Locus Names:Saci_1181Imported
OrganismiSulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Taxonomic identifieri330779 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001018 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei13 – 33HelicalSequence analysisAdd BLAST21
Transmembranei49 – 69HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deletion results in dysregulation of the archaellum regulatory network that leads to reduced motility, though the archaellum is properly assembled. Deletion mutant is still able to sense starvation and to induce archaellum formation, but with a delay at the signal transduction cascade level.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi344K → A: Abolishes autophosphorylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004395101 – 623Serine/threonine-protein kinase ArnSAdd BLAST623

Post-translational modificationi

Autophosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Expressioni

Inductioni

Induced during starvation conditions.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi330779.Saci_1181.

Structurei

3D structure databases

ProteinModelPortaliQ4J9K4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini317 – 623Protein kinasePROSITE-ProRule annotationAdd BLAST307

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiarCOG03682. Archaea.
COG0515. LUCA.
HOGENOMiHOG000134610.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

Q4J9K4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLMSFILLIS TSMILISVFS YVLIALIVLG IVLSLISLLS PRVSYVSSVY
60 70 80 90 100
LIGLTVYLNP GFSLTQSLIN TVPILGEINM LSGLLKTPVT SIISSLFSLA
110 120 130 140 150
LLNLGIKGYE IYLSKHADIV KLDSIIFSQH GLPKQANWNI VVNSEVKLLK
160 170 180 190 200
SNEKLILSNT PEAKYELCPT LVDHKYYVPN KVTGLAFEGE VIRINYSPVD
210 220 230 240 250
GVPYEKFRNC FTVFEVEGLP PDTSFVIEIN GASLKLKDRK FAKLSLEPLY
260 270 280 290 300
WKVNEIRISR EDEEEIYEPD IKEGLTFRGG TVKINFRRKV IRYKTAKIPS
310 320 330 340 350
IENWDPGIWV GQEVYGYRVI EVIGLGGNGY VMKVEKNGLL YAMKVLSVNK
360 370 380 390 400
FTNSLEHFDN LLKESENLEK LSKDPRLVSI YGSFVDKNNI QSALAGDYTS
410 420 430 440 450
YYKYPPAIIM EFMEGGTLFD LISRVDLVQS KYWQYIVKLV IKEIAKALTF
460 470 480 490 500
LHKRGYVHLD VKPQNIFLKE KINGEPEVVY KILSSTPGII KLGDLGSAVR
510 520 530 540 550
VGEKITQATP AYSPPEQIEA VITGKGAQPS MDNYALGVTL YKLLTMKNLD
560 570 580 590 600
YVNYLDKAFD EYIKGDPSIA MKYINMAKMS MVNFKPKLPH NTLPELANVV
610 620
QGTLVVDPKR RLTSYDIVKI LEG
Length:623
Mass (Da):70,061
Last modified:August 2, 2005 - v1
Checksum:i81A9B51DD18E11D7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000077 Genomic DNA. Translation: AAY80528.1.

Genome annotation databases

EnsemblBacteriaiAAY80528; AAY80528; Saci_1181.
KEGGisai:Saci_1181.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000077 Genomic DNA. Translation: AAY80528.1.

3D structure databases

ProteinModelPortaliQ4J9K4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi330779.Saci_1181.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAY80528; AAY80528; Saci_1181.
KEGGisai:Saci_1181.

Phylogenomic databases

eggNOGiarCOG03682. Archaea.
COG0515. LUCA.
HOGENOMiHOG000134610.

Enzyme and pathway databases

BioCyciSACI330779:GH9J-1146-MONOMER.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiARNS_SULAC
AccessioniPrimary (citable) accession number: Q4J9K4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 12, 2017
Last sequence update: August 2, 2005
Last modified: May 10, 2017
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.