Q4J9C6 (OGT_SULAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methylated-DNA--protein-cysteine methyltransferase
EC=2.1.1.63

Alternative name(s):
6-O-methylguanine-DNA methyltransferase
Short name=MGMT
O-6-methylguanine-DNA-alkyltransferase

Gene names

Name:	ogt
Ordered Locus Names:	Saci_1260

Organism

Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) [Complete proteome] [HAMAP]

Taxonomic identifier

330779 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	155 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated By similarity. HAMAP MF_00772
Catalytic activity	DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine. HAMAP MF_00772
Subcellular location	Cytoplasm By similarity HAMAP MF_00772.
Miscellaneous	This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocytalyst that acts repeatedly and over many reaction cycles. HAMAP MF_00772
Sequence similarities	Belongs to the MGMT family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methylated-DNA-[protein]-cysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 155	155	Methylated-DNA--protein-cysteine methyltransferase HAMAP MF_00772		PRO_0000139386
Sites
	Active site	119	1	Nucleophile; methyl group acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4J9C6 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: 2840CF5D08773B1D	FASTA	155	17,556
	10 20 30 40 50 60 MIVYGVYNSP LGTITVAKNE RGIIMLDFCD CAERNLVDNS TFTDLFHKFD NYFQGKPVEF 70 80 90 100 110 120 NETVDLFVNN FRRRVFNEVR RIGWGKVKTY KEIAETLKTS PRAVGMALSK NPVLLIIPCH 130 140 150 RIIAESGLGG FSRGIELKKK LLELEGVNIE ALVRG

References

[1]

"The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota." Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E., Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A. J. Bacteriol. 187:4992-4999(2005) [PubMed: 15995215] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000077 Genomic DNA. Translation: AAY80605.1.
RefSeq	YP_255898.1. NC_007181.1.
3D structure databases
ProteinModelPortal	Q4J9C6.
SMR	Q4J9C6. Positions 1-149.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3473061.
GenomeReviews	Gene locus Saci_1260 in contig CP000077_GR.
KEGG	sai:Saci_1260.
NMPDR	fig|330779.3.peg.2139.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG752398.
OMA	MANSRNP.
ProtClustDB	CLSK803403.
Enzyme and pathway databases
BioCyc	SACI330779:SACI_1260-MONOMER.
Family and domain databases
HAMAP	MF_00772. OGT. [Tree]
InterPro	IPR023546. MethylDNA_cys_MeTrfase. IPR001497. MethylDNA_cys_MeTrfase_AS. IPR014048. MethylDNA_cys_MeTrfase_DNA-bd. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K00567.
Pfam	PF01035. DNA_binding_1. 1 hit. [Graphical view]
SUPFAM	SSF46767. MethylDNA_cys_mtrans_DNA_bd. 1 hit.
TIGRFAMs	TIGR00589. Ogt. 1 hit.
PROSITE	PS00374. MGMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OGT_SULAC

Accession

Primary (citable) accession number: Q4J9C6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
Last sequence update:	August 2, 2005
Last modified:	November 16, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program