Q4J963 (RIFK_SULAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Riboflavin kinase
Short name=RFK
EC=2.7.1.161

Alternative name(s):
CTP-dependent riboflavin kinase
CTP:riboflavin 5'-phosphotransferase
Flavokinase

Gene names

Name:	ribK
Ordered Locus Names:	Saci_1332

Organism

Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) [Complete proteome] [HAMAP]

Taxonomic identifier

330779 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	159 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity. HAMAP MF_01285
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01285
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW riboflavin kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 159	159	Riboflavin kinase HAMAP MF_01285		PRO_0000322078
Regions
	Nucleotide binding	38 – 43	6	CDP By similarity
	Nucleotide binding	139 – 142	4	CDP By similarity
Sites
	Metal binding	67	1	Magnesium By similarity
	Metal binding	69	1	Magnesium By similarity
	Binding site	126	1	FMN By similarity
	Binding site	134	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4J963 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: 0611C0DCCB88BC6C	FASTA	159	17,896
	10 20 30 40 50 60 MIKLTDEGEK MLRECAESLV DLFAKNRIVK IKAKVTSGLG EGRIFVSLPY YMEAFNKFLG 70 80 90 100 110 120 FQPYPGTLNA VIYDRISMEN RLLLDLSRGI LIPEHKEPNR VLGSVKAFPA SINSVSPVAV 130 140 150 VIPTRTTHPK SVVELLSPHK LREKLELEDG DEIEIEVYL

References

[1]

"The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota." Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E., Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A. J. Bacteriol. 187:4992-4999(2005) [PubMed: 15995215] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000077 Genomic DNA. Translation: AAY80667.1.
RefSeq	YP_255960.1. NC_007181.1.
3D structure databases
ProteinModelPortal	Q4J963.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3473445.
GenomeReviews	Gene locus Saci_1332 in contig CP000077_GR.
KEGG	sai:Saci_1332.
NMPDR	fig|330779.3.peg.296.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG553349.
OMA	PYEGTLN.
ProtClustDB	CLSK802937.
Enzyme and pathway databases
BioCyc	SACI330779:SACI_1332-MONOMER.
Family and domain databases
HAMAP	MF_01285. Riboflavin_kinase. [Tree]
InterPro	IPR023470. Riboflavin_kinase_archaeal. IPR023602. Riboflavin_kinase_CTP-dep. IPR023465. Riboflavin_kinase_domain. [Graphical view]
Gene3D	G3DSA:2.40.30.30. Riboflavin_kinase. 1 hit.
KO	K07732.
Pfam	PF01982. CTP-dep_RFKase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIFK_SULAC

Accession

Primary (citable) accession number: Q4J963

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	August 2, 2005
Last modified:	November 16, 2011
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program