Reviewed, UniProtKB/Swiss-Prot Q4J940 (G3P_SULAC)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase
      Short name=GAPDH
    EC=1.2.1.59
Alternative name(s):
    NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase

Gene names

Name:	gap
Ordered Locus Names:	Saci_1356

Organism

Sulfolobus acidocaldarius [Complete proteome] [HAMAP]

Taxonomic identifier

2285 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H. HAMAP MF_00559
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. HAMAP MF_00559
Subunit structure	Homotetramer By similarity. HAMAP MF_00559
Subcellular location	Cytoplasm By similarity HAMAP MF_00559.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NAD(P)+) (phosphorylating) activity Inferred from electronic annotation. Source: EC glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	Glyceraldehyde-3-phosphate dehydrogenase HAMAP MF_00559		PRO_0000145734
Regions
	Nucleotide binding	13 – 14	2	NAD By similarity
	Region	140 – 142	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	195 – 196	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	141	1	Nucleophile By similarity
	Binding site	111	1	NAD; via amide nitrogen By similarity
	Binding site	169	1	NAD By similarity
	Binding site	303	1	NAD; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4J940-1 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: D0F30CB7AAF27CD2	FASTA	343	37,957
	10 20 30 40 50 60 MVKVKVAING YGTIGKRVAD AIRLQPDMEL IGVAKTSPNY EAFTAMRKGY KLYTTKENIQ 70 80 90 100 110 120 KFQNSGINVA GSIEDMIKDS DIIIDATPGG VGANYKKIYQ EYGKKAIFQG GEKSDVADVS 130 140 150 160 170 180 FSALCNYNDA INKNYIRVVS CNTTGILRVL CTINNFDKIE KVRGTIVRRA ADPKEVKKGP 190 200 210 220 230 240 INSIVADPAR IPSHHAKDVL TVLKGIDIIT SALVAPTTLM HLHTLFITTR NKVSKEDLLN 250 260 270 280 290 300 ILSNTPRILL LNTEKADAES TAEIMEIARD LGRYRNDVPE TVIFEDSIYT NGNEIFLMYG 310 320 330 340 VHQESIVVPE NIDAIRASLN IMSRDESIRL TNETLKIGKG YLI

References

[1]

"The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota." Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E., Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A. J. Bacteriol. 187:4992-4999(2005) [PubMed: 15995215] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33909 / DSM 639 / IFO 15157 / JCM 8929 / NCIB 11770.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000077 Genomic DNA. Translation: AAY80691.1.
RefSeq	YP_255984.1.
3D structure databases
SMR	Q4J940. Positions 4-343.
ModBase	Search...
Genome annotation databases
GeneID	3473469.
GenomeReviews	Gene locus Saci_1356 in contig CP000077_GR.
KEGG	sai:Saci_1356.
NMPDR	fig|330779.3.peg.122.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG392099.
OMA	VHQESDV.
Enzyme and pathway databases
BioCyc	SACI330779:SACI_1356-MONOMER.
BRENDA	1.2.1.59. 434.
Family and domain databases
HAMAP	MF_00559. G3P_dehdrog_arch. [Tree]
InterPro	IPR020830. GlycerAld_3-P_DH_AS. IPR020829. GlycerAld_3-P_DH_cat. IPR020831. GlycerAld_3-P_DH_family. IPR020828. GlycerAld_3-P_DH_NAD(P)_bd. IPR006436. Glyceraldehyde-3-P_DH_2_arc. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
SMART	SM00846. Gp_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01546. GAPDH-II_archae. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3P_SULAC

Accession

Primary (citable) accession number: Q4J940

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
Last sequence update:	August 2, 2005
Last modified:	February 9, 2010
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)