Q4J8J7 (SYL2_SULAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Leucine--tRNA ligase 2
EC=6.1.1.4

Alternative name(s):
Leucyl-tRNA synthetase 2
Short name=LeuRS 2

Gene names

Name:	leuS2
Ordered Locus Names:	Saci_1572

Organism

Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) [Complete proteome] [HAMAP]

Taxonomic identifier

330779 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	942 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP MF_00049_A
Subcellular location	Cytoplasm HAMAP MF_00049_A.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.
Sequence caution	The sequence AAY80885.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	leucyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW leucine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 942	942	Leucine--tRNA ligase 2 HAMAP MF_00049_A		PRO_0000152143
Regions
	Motif	35 – 45	11	"HIGH" region HAMAP MF_00049_A
	Motif	619 – 623	5	"KMSKS" region HAMAP MF_00049_A
Sites
	Binding site	622	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4J8J7 [UniParc]. Last modified September 27, 2005. Version 2. Checksum: 4DD802C8C8C861C4	FASTA	942	109,179
	10 20 30 40 50 60 MKISEKWQKE WENKRVFDAT PDPNKKKFFT TIAFPYPNSP FHLGHGRTYV TGDIYARYMR 70 80 90 100 110 120 MRGYNVLFPM AFHYTGTPII AMADDVAKGD KELIDIFKSI YEIPDDVISK LVDPLFMANY 130 140 150 160 170 180 FKEEIKQAMK EIGLSIDWRR EFTTIDPEFS SFIIWQFRKL QEKGFIVRDT HPVGWCPVHH 190 200 210 220 230 240 IPVGMHDTKG DMEPEIGEFV LIYFDSDMGI LPVATLRPET VFGAIAVWVN PHESYSIVEI 250 260 270 280 290 300 DGKKYVMSEK ASSKLSFQID NLKVITVVKG SELVKHSAVN PITGKEVPII GANFVDPLTG 310 320 330 340 350 360 TGVVMSVPAH APFDYFYLKK TKSELSIISV IRVEGMGETL AKDLVEKSNP QNDNDLKKLT 370 380 390 400 410 420 EQVYRIEYNK GVMIDITKLV KPEYVEELKP LVNLPVPAAR QKITEFITQK GLGRKIYEIM 430 440 450 460 470 480 NRPVYCRCGN EVVVKILKDQ WFLDYGNQEW KDLARKSIES IRFIPPEIKK DFEFVVDWLQ 490 500 510 520 530 540 KRACARTRGL GTPLPWDKKW IIESLSDSTI YMAFYTIAHR LKEHKLKPSQ LTYEFWEYIM 550 560 570 580 590 600 LGNGNPDEIS KISGIPVEVI KAMRDEFLYW YPLDVRHSGK DLVPNHLSFF IFNHAAIFPH 610 620 630 640 650 660 QLWPKGIAVN GFVLYDGKKM SKSLRNIVPL RKAIRMYSPD VIRIALTTNA DIGSDVNFSD 670 680 690 700 710 720 SYAKSIIDTL KNYYDLLEKL KEFKGEDEGF PEKWLKSKFY QMVINVTQYM DSLDLRSSSN 730 740 750 760 770 780 EILYNFSSYI NEYFELVRSE GREPNGKLLS QILQIWIKLL SPFAPHFAEE LWHKIGKNTL 790 800 810 820 830 840 VSLESWPIID QSNVDLFIDL THTYHRKLLN DIQAILSVYK DTPKSIKIFV ANKEFLNVLR 850 860 870 880 890 900 DAINIVQKGG QLRQLMEIHK PKGKQDARLY QKIYEEAREI DDDMKKLVTN FDFDEKDLLD 910 920 930 940 KGLKYLSYKL GIKEIRILDA SEMDRTKYNK DALPLRPAII IE

References

[1]

"The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota." Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E., Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A. J. Bacteriol. 187:4992-4999(2005) [PubMed: 15995215] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000077 Genomic DNA. Translation: AAY80885.1. Different initiation.
RefSeq	YP_256178.1. NC_007181.1.
3D structure databases
ProteinModelPortal	Q4J8J7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3474607.
GenomeReviews	Gene locus Saci_1572 in contig CP000077_GR.
KEGG	sai:Saci_1572.
NMPDR	fig|330779.3.peg.378.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG445334.
PhylomeDB	Q4J8J7.
ProtClustDB	PRK12300.
Enzyme and pathway databases
BioCyc	SACI330779:SACI_1572-MONOMER.
Family and domain databases
HAMAP	MF_00049_A. Leu_tRNA_synth_A. [Tree]
InterPro	IPR002300. aa-tRNA-synth_Ia. IPR020791. Leu-tRNA-synth_Ia_arc. IPR004493. Leu-tRNA-synth_Ia_arc/euk. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
Gene3D	G3DSA:3.90.740.10. G3DSA:3.90.740.10. 2 hits. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01869.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00395. LeuS_arch. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYL2_SULAC

Accession

Primary (citable) accession number: Q4J8J7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
Last sequence update:	September 27, 2005
Last modified:	January 25, 2012
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program