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Q4J8J7 (SYL2_SULAC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase 2

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase 2
Short name=LeuRS 2
Gene names
Name:leuS2
Ordered Locus Names:Saci_1572
OrganismSulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) [Complete proteome] [HAMAP]
Taxonomic identifier330779 [NCBI]
Taxonomic lineageArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus

Protein attributes

Sequence length942 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence caution

The sequence AAY80885.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 942942Leucine--tRNA ligase 2 HAMAP-Rule MF_00049
PRO_0000152143

Regions

Motif35 – 4511"HIGH" region HAMAP-Rule MF_00049
Motif619 – 6235"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6221ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4J8J7 [UniParc].

Last modified September 27, 2005. Version 2.
Checksum: 4DD802C8C8C861C4

FASTA942109,179
        10         20         30         40         50         60 
MKISEKWQKE WENKRVFDAT PDPNKKKFFT TIAFPYPNSP FHLGHGRTYV TGDIYARYMR 

        70         80         90        100        110        120 
MRGYNVLFPM AFHYTGTPII AMADDVAKGD KELIDIFKSI YEIPDDVISK LVDPLFMANY 

       130        140        150        160        170        180 
FKEEIKQAMK EIGLSIDWRR EFTTIDPEFS SFIIWQFRKL QEKGFIVRDT HPVGWCPVHH 

       190        200        210        220        230        240 
IPVGMHDTKG DMEPEIGEFV LIYFDSDMGI LPVATLRPET VFGAIAVWVN PHESYSIVEI 

       250        260        270        280        290        300 
DGKKYVMSEK ASSKLSFQID NLKVITVVKG SELVKHSAVN PITGKEVPII GANFVDPLTG 

       310        320        330        340        350        360 
TGVVMSVPAH APFDYFYLKK TKSELSIISV IRVEGMGETL AKDLVEKSNP QNDNDLKKLT 

       370        380        390        400        410        420 
EQVYRIEYNK GVMIDITKLV KPEYVEELKP LVNLPVPAAR QKITEFITQK GLGRKIYEIM 

       430        440        450        460        470        480 
NRPVYCRCGN EVVVKILKDQ WFLDYGNQEW KDLARKSIES IRFIPPEIKK DFEFVVDWLQ 

       490        500        510        520        530        540 
KRACARTRGL GTPLPWDKKW IIESLSDSTI YMAFYTIAHR LKEHKLKPSQ LTYEFWEYIM 

       550        560        570        580        590        600 
LGNGNPDEIS KISGIPVEVI KAMRDEFLYW YPLDVRHSGK DLVPNHLSFF IFNHAAIFPH 

       610        620        630        640        650        660 
QLWPKGIAVN GFVLYDGKKM SKSLRNIVPL RKAIRMYSPD VIRIALTTNA DIGSDVNFSD 

       670        680        690        700        710        720 
SYAKSIIDTL KNYYDLLEKL KEFKGEDEGF PEKWLKSKFY QMVINVTQYM DSLDLRSSSN 

       730        740        750        760        770        780 
EILYNFSSYI NEYFELVRSE GREPNGKLLS QILQIWIKLL SPFAPHFAEE LWHKIGKNTL 

       790        800        810        820        830        840 
VSLESWPIID QSNVDLFIDL THTYHRKLLN DIQAILSVYK DTPKSIKIFV ANKEFLNVLR 

       850        860        870        880        890        900 
DAINIVQKGG QLRQLMEIHK PKGKQDARLY QKIYEEAREI DDDMKKLVTN FDFDEKDLLD 

       910        920        930        940 
KGLKYLSYKL GIKEIRILDA SEMDRTKYNK DALPLRPAII IE 

« Hide

References

[1]"The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota."
Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E., Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A.
J. Bacteriol. 187:4992-4999(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000077 Genomic DNA. Translation: AAY80885.1. Different initiation.
RefSeqYP_256178.1. NC_007181.1.

3D structure databases

ProteinModelPortalQ4J8J7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING330779.Saci_1572.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAY80885; AAY80885; Saci_1572.
GeneID3474607.
KEGGsai:Saci_1572.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000216620.
KOK01869.

Enzyme and pathway databases

BioCycSACI330779:GH9J-1544-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_A. Leu_tRNA_synth_A.
InterProIPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsTIGR00395. leuS_arch. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYL2_SULAC
AccessionPrimary (citable) accession number: Q4J8J7
Entry history
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: September 27, 2005
Last modified: May 14, 2014
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries