Q4J8F8 (PURL_SULAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	Saci_1610

Organism

Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) [Complete proteome] [HAMAP]

Taxonomic identifier

330779 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	710 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 710	710	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_0000100524
Regions
	Nucleotide binding	95 – 106	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q4J8F8 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: DDE5D46E187F5075	FASTA	710	77,842
	10 20 30 40 50 60 MRLSLSSQEM ELVRKFLGRE PKEEEWLVVD ALWSEHCSYK SSKIFLRSFP SEGERVVMGI 70 80 90 100 110 120 EDWQDAGALD VGDGWAVVLK LESHNHPSAI DPFNGAATGI GGIIRDIISK GARPIALLDM 130 140 150 160 170 180 IRVGNLNNSR NRWLLKNIIA GIGFYGNSIG VPVVAGELAF DETYNDNPLV DVAGLGVVKK 190 200 210 220 230 240 DKIVPSVVKE AGLKIVLVGL TGLDGLGGAS FASRKLSGED EIGAVQIADP FAGKIVLDVT 250 260 270 280 290 300 LQIADKVEAI KDLGGGGLVV GITEMANGLG VEVELDKIPL RVKDLTPGEI LVSETQERMV 310 320 330 340 350 360 FAVKPERVKE VCEAFEYYEY PCAVIGEFTN DTSIRFLYKG KEVVNLPSSL LLNPPRYKWG 370 380 390 400 410 420 VKKTKYSVYY EKPNINLEVA VKEILSYPDL VSKFWAYSQF DYEVGTSTVL KPGEADSGLI 430 440 450 460 470 480 SLPNGKLLAI KGDANPDLCA EDSYECGRYI VAEAYRNLAT VGAKGIGVVD HLQFGDPKKP 490 500 510 520 530 540 EVYYQFVEAV RGIAEASKYF GTPIVGGKVS FYNENKEGKP IKPTPLVVMA GLVQDKFLRP 550 560 570 580 590 600 KITEGASIIM IGFTREEMGG SLLAKIFGNY GDVPKTRLNE ELLSSELVIK AINDGKIIFA 610 620 630 640 650 660 KDISKGGLVG ALLPILVRGF GVSIDTNLIP SDTDDVISKL FSENGGRFIV LSEREDDINW 670 680 690 700 710 LESQSRGIYV SKIGEVTREQ YVMWLREGKK IDLTKEVNNY HRYLEEVTSD

References

[1]

"The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota." Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E., Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A. J. Bacteriol. 187:4992-4999(2005) [PubMed: 15995215] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000077 Genomic DNA. Translation: AAY80922.1.
RefSeq	YP_256215.1. NC_007181.1.
3D structure databases
ProteinModelPortal	Q4J8F8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3474292.
GenomeReviews	Gene locus Saci_1610 in contig CP000077_GR.
KEGG	sai:Saci_1610.
NMPDR	fig|330779.3.peg.526.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG311214.
OMA	YGNSFGV.
PhylomeDB	Q4J8F8.
ProtClustDB	PRK01213.
Enzyme and pathway databases
BioCyc	SACI330779:SACI_1610-MONOMER.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_SULAC

Accession

Primary (citable) accession number: Q4J8F8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
Last sequence update:	August 2, 2005
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program