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Protein

Glyceraldehyde dehydrogenase medium chain

Gene

cutB

Organism
Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the glyceraldehyde dehydrogenase which is involved the nonphosphorylated Entner-Doudoroff pathway. Catalyzes the oxidation of D-glyceraldehyde to yield glycerate. When the artificial electron acceptor 2,6-dichlorophenol-indophenol (Cl2Ind) is used, the enzyme shows a broad substrate range (glyceraldehyde-3-phosphate, formaldehyde, acetaldehyde, propionaldehyde and isobutyraldehyde), but is most active with D-glyceraldehyde. It is not known which acceptor is utilized in vivo.1 Publication

Catalytic activityi

D-glyceraldehyde + H2O + acceptor = D-glycerate + reduced acceptor.1 Publication

Cofactori

FAD1 PublicationNote: Binds 1 FAD per subunit.1 Publication

Kineticsi

  1. KM=30 µM for propionaldehyde (at 80 degrees Celsius and pH 6.7)1 Publication
  2. KM=90 µM for D,L-glyceraldehyde (at 80 degrees Celsius and pH 6.7)1 Publication

    pH dependencei

    Optimum pH is 6.7. At pH 6.7, glyceraldehyde is the predominant substrate, however at pH 7.5 the dehydrogenase exhibits activity preferentially towards the aliphatic aldehydes such as formaldehyde, acetaldehyde and propionaldehyde.1 Publication

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi31 – 35FADBy similarity5
    Nucleotide bindingi110 – 114FADBy similarity5

    GO - Molecular functioni

    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glyceraldehyde dehydrogenase medium chain (EC:1.2.99.8)
    Alternative name(s):
    Glyceraldehyde dehydrogenase subunit B
    Glyceraldehyde dehydrogenase subunit beta
    Gene namesi
    Name:cutB
    Ordered Locus Names:Saci_2269
    OrganismiSulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
    Taxonomic identifieri330779 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
    Proteomesi
    • UP000001018 Componenti: Chromosome

    Subcellular locationi

    • Cytoplasm 1 Publication

    GO - Cellular componenti

    • cytoplasm Source: UniProtKB
    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004242721 – 281Glyceraldehyde dehydrogenase medium chainAdd BLAST281

    Interactioni

    Subunit structurei

    Heterotrimer composed of a large chain (CutA), a medium chain (CutB) and a small chain (CutC).1 Publication

    Protein-protein interaction databases

    STRINGi330779.Saci_2269.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4J6M6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini1 – 176FAD-binding PCMH-typePROSITE-ProRule annotationAdd BLAST176

    Sequence similaritiesi

    Contains 1 FAD-binding PCMH-type domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiarCOG01926. Archaea.
    COG1319. LUCA.
    HOGENOMiHOG000244728.
    KOiK18021.
    OMAiAMLNMRL.

    Family and domain databases

    Gene3Di3.30.43.10. 1 hit.
    3.30.465.10. 1 hit.
    InterProiIPR005107. CO_DH_flav_C.
    IPR016169. CO_DH_flavot_FAD-bd_sub2.
    IPR016166. FAD-bd_2.
    IPR016167. FAD-bd_2_sub1.
    IPR002346. Mopterin_DH_FAD-bd.
    [Graphical view]
    PfamiPF03450. CO_deh_flav_C. 1 hit.
    PF00941. FAD_binding_5. 1 hit.
    [Graphical view]
    SMARTiSM01092. CO_deh_flav_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF55447. SSF55447. 1 hit.
    SSF56176. SSF56176. 1 hit.
    PROSITEiPS51387. FAD_PCMH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q4J6M6-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MYPPEFSYVR AESLQEALKF LEGNDNTRPL AGGQSLIPML KLRVLSPDYI
    60 70 80 90 100
    LDINRLNELN YVKTSLNGVS IGALTRYHDI LSNDIVKSKV PLMHHATRTI
    110 120 130 140 150
    GDMQVRNMGT IGGAISNADP ASDMPVVLTA LNATIILSSA SGSRSVKALD
    160 170 180 190 200
    FFKGPFTTDT NKGELVTQIE VPVLDGYKTV YKKVVRRAGD YALASVALAI
    210 220 230 240 250
    KLKGNEIEDI KLAYGGVHDK PFRAMEVEKN VIGKKLNDDL VKDIASKVSS
    260 270 280
    QINPPSDHRG SSWYRREVVK VLTMKAFKEV A
    Length:281
    Mass (Da):30,863
    Last modified:August 2, 2005 - v1
    Checksum:i9F34004616E96EC6
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP000077 Genomic DNA. Translation: AAY81555.1.
    RefSeqiWP_011279057.1. NC_007181.1.

    Genome annotation databases

    EnsemblBacteriaiAAY81555; AAY81555; Saci_2269.
    GeneIDi3474530.
    KEGGisai:Saci_2269.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP000077 Genomic DNA. Translation: AAY81555.1.
    RefSeqiWP_011279057.1. NC_007181.1.

    3D structure databases

    ProteinModelPortaliQ4J6M6.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi330779.Saci_2269.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAY81555; AAY81555; Saci_2269.
    GeneIDi3474530.
    KEGGisai:Saci_2269.

    Phylogenomic databases

    eggNOGiarCOG01926. Archaea.
    COG1319. LUCA.
    HOGENOMiHOG000244728.
    KOiK18021.
    OMAiAMLNMRL.

    Family and domain databases

    Gene3Di3.30.43.10. 1 hit.
    3.30.465.10. 1 hit.
    InterProiIPR005107. CO_DH_flav_C.
    IPR016169. CO_DH_flavot_FAD-bd_sub2.
    IPR016166. FAD-bd_2.
    IPR016167. FAD-bd_2_sub1.
    IPR002346. Mopterin_DH_FAD-bd.
    [Graphical view]
    PfamiPF03450. CO_deh_flav_C. 1 hit.
    PF00941. FAD_binding_5. 1 hit.
    [Graphical view]
    SMARTiSM01092. CO_deh_flav_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF55447. SSF55447. 1 hit.
    SSF56176. SSF56176. 1 hit.
    PROSITEiPS51387. FAD_PCMH. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiCUTB_SULAC
    AccessioniPrimary (citable) accession number: Q4J6M6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 13, 2013
    Last sequence update: August 2, 2005
    Last modified: November 2, 2016
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.