Reviewed, UniProtKB/Swiss-Prot Q4IJP1 (DOT1_GIBZE)

Names and origin

Protein names

Recommended name:
    Histone-lysine N-methyltransferase, H3 lysine-79 specific
    EC=2.1.1.43
Alternative name(s):
    Histone H3-K79 methyltransferase
    H3-K79-HMTase

Gene names

Name:	DOT1
ORF Names:	FG02567

Organism

Gibberella zeae (Fusarium graminearum)

Taxonomic identifier

5518 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Hypocreomycetidae › Hypocreales › Nectriaceae › Gibberella

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Histone methyltransferase that specifically methylates histone H3 to form H3K79me. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histones By similarity.
Catalytic activity	S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine.
Enzyme regulation	Ubiquitination of histone H2B to form H2BK123ub1 is required for efficient DOT1 methyltransferase activity on histone H3 By similarity.
Subcellular location	Nucleus By similarity.
Miscellaneous	In contrast to other lysine histone methyltransferase, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.
Sequence similarities	Belongs to the DOT1 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat
Ligand	S-adenosyl-L-methionine
Molecular function	Chromatin regulator Methyltransferase Transferase
Gene Ontology (GO)
Biological process	chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	histone-lysine N-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 493	493	Histone-lysine N-methyltransferase, H3 lysine-79 specific		PRO_0000270613
Regions
	Region	337 – 339	3	S-adenosyl-L-methionine binding By similarity
	Region	397 – 398	2	S-adenosyl-L-methionine binding By similarity
	Motif	333 – 344	12	SAM-binding motif 1 By similarity
	Motif	412 – 421	10	SAM-binding motif 2 By similarity
Sites
	Binding site	314	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	361	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4IJP1-1 [UniParc]. Last modified August 16, 2005. Version 1. Checksum: D41F5E293FF3475C	FASTA	493	55,587
	10 20 30 40 50 60 MRLFGGKNNK FKVDPPKIRI EKVVVDRPAQ KPKPKPNPAL SGSASRSSSS HHPSPKPLAR 70 80 90 100 110 120 QASHSPYPSS CDEKRLERKR KAPSVSRKSP ASDRIEFDKD SDGEDDGWMT LDTKRQRKGT 130 140 150 160 170 180 EDSGFVDPNR KLRSVRAFEE RMDSRKFIHA VDVASLEHKC VPVMGAQKDE VAIRLQYPSL 190 200 210 220 230 240 QPREKYELVW GKDKIDAVEA SIKVVRHVAE TYLTEEEAEP FTNPNGGIIR RLEKASNRNI 250 260 270 280 290 300 QDLMGFKAAL REYNEKLRAL VDDGVIAKNL DKMHELPQHL VAFILDQIYD RTVALKVELL 310 320 330 340 350 360 SKYENGTDYV YGELLHPFIS KVLVEQTRMT SGQVFVDLGS GVGNVVLQAA LEIGCESWGC 370 380 390 400 410 420 EMMENACNLA EEQKKEFDAR CMLWGVRPGK VHLERGDFRK NAPIHEALKR ADVVLVNNKA 430 440 450 460 470 480 FTSQLNDDLV RMFLDLKSGC KVVSLKSFVA EKSNNHNIND VGSTILEVEE CIYPEGYVSW 490 TNAGGSYFIS TRK

References

[1]

"The Fusarium graminearum genome reveals a link between localized polymorphism and pathogen specialization." Cuomo C.A., Gueldener U., Xu J.-R., Trail F., Turgeon B.G., Di Pietro A., Walton J.D., Ma L.-J., Baker S.E., Rep M., Adam G., Antoniw J., Baldwin T., Calvo S.E., Chang Y.-L., DeCaprio D., Gale L.R., Gnerre S. , Goswami R.S., Hammond-Kosack K., Harris L.J., Hilburn K., Kennell J.C., Kroken S., Magnuson J.K., Mannhaupt G., Mauceli E.W., Mewes H.-W., Mitterbauer R., Muehlbauer G., Muensterkoetter M., Nelson D., O'Donnell K., Ouellet T., Qi W., Quesneville H., Roncero M.I.G., Seong K.-Y., Tetko I.V., Urban M., Waalwijk C., Ward T.J., Yao J., Birren B.W., Kistler H.C. Science 317:1400-1402(2007) [PubMed: 17823352] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PH-1 / NRRL 31084.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AACM01000128 Genomic DNA. Translation: EAA68810.1.
RefSeq	XP_382743.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2784179.
KEGG	fgr:FG02567.1.
Enzyme and pathway databases
BRENDA	2.1.1.43. 74326.
Family and domain databases
InterPro	IPR013110. DOT1. [Graphical view]
Pfam	PF08123. DOT1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DOT1_GIBZE

Accession

Primary (citable) accession number: Q4IJP1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	August 16, 2005
Last modified:	June 16, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)