Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA wybutosine-synthesizing protein 4

Gene

PPM2

Organism
Gibberella zeae (strain PH-1 / ATCC MYA-4620 / FGSC 9075 / NRRL 31084) (Wheat head blight fungus) (Fusarium graminearum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Probable S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. May methylate the carboxyl group of leucine residues to form alpha-leucine ester residues (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + 7-((3S)-3-amino-3-carboxypropyl)wyosine(37) in tRNA(Phe) = S-adenosyl-L-homocysteine + 7-((3S)-3-amino-3-(methoxycarbonyl)propyl)wyosine(37) in tRNA(Phe).
S-adenosyl-L-methionine + 7-((3S)-3-amino-3-(methoxycarbonyl)propyl)wyosine(37) in tRNA(Phe) + CO2 = S-adenosyl-L-homocysteine + wybutosine(37) in tRNA(Phe).

Pathwayi: wybutosine-tRNA(Phe) biosynthesis

This protein is involved in the pathway wybutosine-tRNA(Phe) biosynthesis, which is part of tRNA modification.
View all proteins of this organism that are known to be involved in the pathway wybutosine-tRNA(Phe) biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei68 – 681S-adenosyl-L-methionineBy similarity
Binding sitei90 – 901S-adenosyl-L-methionineBy similarity
Binding sitei166 – 1661S-adenosyl-L-methionine; via carbonyl oxygenBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00375.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA wybutosine-synthesizing protein 4 (EC:2.1.1.290, EC:2.3.1.231)
Short name:
tRNA-yW synthesizing protein 4
Alternative name(s):
Leucine carboxyl methyltransferase 2
tRNA(Phe) (7-(3-amino-3-(methoxycarbonyl)propyl)wyosine(37)-N)-methoxycarbonyltransferase
tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-O)-methyltransferase
Gene namesi
Name:PPM2
Synonyms:TYW4
ORF Names:FGRRES_04298, FGSG_04298
OrganismiGibberella zeae (strain PH-1 / ATCC MYA-4620 / FGSC 9075 / NRRL 31084) (Wheat head blight fungus) (Fusarium graminearum)
Taxonomic identifieri229533 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesNectriaceaeFusarium
Proteomesi
  • UP000070720 Componenti: Chromosome 2
  • UP000009057 Componenti: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 989989tRNA wybutosine-synthesizing protein 4PRO_0000226142Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi229533.XP_384474.1.

Structurei

3D structure databases

ProteinModelPortaliQ4IER0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini794 – 948155JmjCPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni139 – 1402S-adenosyl-L-methionine bindingBy similarity

Sequence similaritiesi

Contains 1 JmjC domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2132. Eukaryota.
KOG2918. Eukaryota.
ENOG410YB9K. LUCA.
InParanoidiQ4IER0.
KOiK15451.
OrthoDBiEOG092C0IJS.

Family and domain databases

Gene3Di2.120.10.80. 1 hit.
3.40.50.150. 2 hits.
InterProiIPR003347. JmjC_dom.
IPR015915. Kelch-typ_b-propeller.
IPR007213. Ppm1/Ppm2/Tcmp.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR13600. PTHR13600. 1 hit.
SMARTiSM00558. JmjC. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51184. JMJC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4IER0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKEKPSSTR VAKTQALDDL IMGTNSSSIV SKRSVERLYY PNELHFFRYF
60 70 80 90 100
VNKFQRRAPL INRGYWLRLR AIDSRVEERW SLISGQEDVD YPDLMRKKRA
110 120 130 140 150
IVLGTPQLSE LLGEDPYVSE KETDHLLLRS DKYCQVGCDL RELETLRKCL
160 170 180 190 200
ESFLPLFECS VLFVAEVSVT YMDTISADAL IQWASYIGQA EFCLLEQILP
210 220 230 240 250
HGPDHPFAST MLKHFNKLNT SLKSVHQYPT VNSQRSRFEK RGWNSVDVWD
260 270 280 290 300
LWEAWNSEVF LNSSERAALD DVEPFDEWEE FILFARHYIV LHATSYHRGG
310 320 330 340 350
KGAGQQMLAS PPGKHVKANM VATKSLGAPK RRFGSPLVAS SPEGGQYLVH
360 370 380 390 400
TLGMGINARL DSCDIYSIQE SSTSLEMAPV GPSARICHTT TDLGQGDLLL
410 420 430 440 450
VGGRASPSKA FSDCWVLKKS SNSWEKTFDL PVPLFRHSTV NLPGSSLALV
460 470 480 490 500
LGGKTGPSEI SSDYFIFHPV RGWLKCAVSG VSPNSTFGAF AIASTSLGSK
510 520 530 540 550
NGHFEGLLAG GIDGEGKINN QAYFWTVDVT THEPIIHFES VQNYDQHSWA
560 570 580 590 600
LSVFGAQTAN IESLSFVCGG VGQDPSSQGQ SMACLTVKGK SLAVHLVDLG
610 620 630 640 650
EKAGQLPFMV GSATVSSGSQ LVIVGGGATC FSMGTFWDTG VYKIDLEGVV
660 670 680 690 700
SEMIPGRTIH NKPVVVGYQN SPKLTQPTTD GVYPGPQSNA FITAIPKIKL
710 720 730 740 750
QSKSDFEKLV QNRKPVIIEG LDLGGCVEKW NSEYVIQSVG ETKEVVVHEC
760 770 780 790 800
QTSTGKMDFN SKNFRYVTES FSSFMTKAAR GEALYLRALS EEKPTEAPAN
810 820 830 840 850
LAEDFPGLAD DFRLPEELGS VKDRMFSSVL RISGRANMWL HYDVMANVYT
860 870 880 890 900
QIQGSKRMVL FPPTDVSHLA FAPGASSSSL DIFSALDTHQ LASTNPHEAF
910 920 930 940 950
LNPGDLLFIP AMWFHTASPV TDLSVAVNVF FRDLESGYST GRDVYGNRDL
960 970 980
AAYEKGRQDI SRITKSFDRL PSEIRQFYLR RLADELLHE
Length:989
Mass (Da):109,484
Last modified:August 16, 2005 - v1
Checksum:i569239E91EF231E4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS231664 Genomic DNA. Translation: ESU08819.1.
HG970333 Genomic DNA. Translation: CEF79277.1.
RefSeqiXP_011321318.1. XM_011323016.1.

Genome annotation databases

GeneIDi23551556.
KEGGifgr:FGSG_04298.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS231664 Genomic DNA. Translation: ESU08819.1.
HG970333 Genomic DNA. Translation: CEF79277.1.
RefSeqiXP_011321318.1. XM_011323016.1.

3D structure databases

ProteinModelPortaliQ4IER0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi229533.XP_384474.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi23551556.
KEGGifgr:FGSG_04298.

Phylogenomic databases

eggNOGiKOG2132. Eukaryota.
KOG2918. Eukaryota.
ENOG410YB9K. LUCA.
InParanoidiQ4IER0.
KOiK15451.
OrthoDBiEOG092C0IJS.

Enzyme and pathway databases

UniPathwayiUPA00375.

Family and domain databases

Gene3Di2.120.10.80. 1 hit.
3.40.50.150. 2 hits.
InterProiIPR003347. JmjC_dom.
IPR015915. Kelch-typ_b-propeller.
IPR007213. Ppm1/Ppm2/Tcmp.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR13600. PTHR13600. 1 hit.
SMARTiSM00558. JmjC. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51184. JMJC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTYW4_GIBZE
AccessioniPrimary (citable) accession number: Q4IER0
Secondary accession number(s): A0A098DLG7, A0A0E0S702, V6R3K6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: August 16, 2005
Last modified: September 7, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.