Reviewed, UniProtKB/Swiss-Prot Q4I8X0 (TRM10_GIBZE)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(1)-)-methyltransferase
    EC=2.1.1.31
Alternative name(s):
    tRNA methyltransferase 10

Gene names

Name:	TRM10
ORF Names:	FG06338

Organism

Gibberella zeae (Wheat head blight fungus) (Fusarium graminearum)

Taxonomic identifier

5518 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Hypocreomycetidae › Hypocreales › Nectriaceae › Gibberella

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of N(1)-methylguanine at position 9 (m1G9) in cytoplasmic tRNAs By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(1)-methylguanine.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the RNA methyltransferase trmD family. TRM10 subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm Nucleus
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 407	407	tRNA (guanine-N(1)-)-methyltransferase		PRO_0000060516

Sequences

	Sequence		Length	Mass (Da)
	Q4I8X0-1 [UniParc]. Last modified August 16, 2005. Version 1. Checksum: 30151BC819DC5C24	FASTA	407	46,769
	10 20 30 40 50 60 MDLDPAHKPS QAEETKEQGN EQGQVEQNQA QQDPTTGPQA ETEKAIIPSQ GSTIPQKRSA 70 80 90 100 110 120 EDEPAQPMSK NALKRLRKQQ QWEAGKEDRK LKRKDSRIAR KVRKREERDA LIAQGINPYA 130 140 150 160 170 180 NKQKPPSVNV PISLIFDCEF EQYMREKEII SLGSQITRSY SENKNAKYRT NIYVSNWNGK 190 200 210 220 230 240 LAERFHQILD DKHQNWKGID FVEGDFIECA EKAREKMKHE NMIEPLQRSL TEKSPWARDE 250 260 270 280 290 300 KDPLPLPDPE PEPRPEYSDI VYLSSDSPYT LERLEPNTSY VIGGLVDKNR EKGLCYKRAR 310 320 330 340 350 360 ERGIRTARLP IGQYMVMQSR TVLTTNHVVE IMLKWLEYEN WGEAFMSVIP KRKGGKLKEQ 370 380 390 400 QGASGETQET EEAEAEDPEE ENEETKDPDA EASASKQNTP KVEVTSK

References

[1]

"The Fusarium graminearum genome reveals a link between localized polymorphism and pathogen specialization." Cuomo C.A., Gueldener U., Xu J.-R., Trail F., Turgeon B.G., Di Pietro A., Walton J.D., Ma L.-J., Baker S.E., Rep M., Adam G., Antoniw J., Baldwin T., Calvo S.E., Chang Y.-L., DeCaprio D., Gale L.R., Gnerre S. , Goswami R.S., Hammond-Kosack K., Harris L.J., Hilburn K., Kennell J.C., Kroken S., Magnuson J.K., Mannhaupt G., Mauceli E.W., Mewes H.-W., Mitterbauer R., Muehlbauer G., Muensterkoetter M., Nelson D., O'Donnell K., Ouellet T., Qi W., Quesneville H., Roncero M.I.G., Seong K.-Y., Tetko I.V., Urban M., Waalwijk C., Ward T.J., Yao J., Birren B.W., Kistler H.C. Science 317:1400-1402(2007) [PubMed: 17823352] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PH-1 / NRRL 31084.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AACM01000254 Genomic DNA. Translation: EAA74955.1.
RefSeq	XP_386514.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2786641.
KEGG	fgr:FG06338.1.
Enzyme and pathway databases
BRENDA	2.1.1.31. 74326.
Family and domain databases
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR007356. tRNA_m1G_MeTrfase_euk. [Graphical view]
PANTHER	PTHR13563. DUF425. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRM10_GIBZE

Accession

Primary (citable) accession number: Q4I8X0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	August 16, 2005
Last modified:	September 22, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)