Q4I061 (NMT_GIBZE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycylpeptide N-tetradecanoyltransferase
EC=2.3.1.97

Alternative name(s):
Myristoyl-CoA:protein N-myristoyltransferase
Short name=NMT
Peptide N-myristoyltransferase

Gene names

Name:	NMT1
ORF Names:	FG09397

Organism

Gibberella zeae (strain PH-1 / ATCC MYA-4620 / FGSC 9075 / NRRL 31084) (Wheat head blight fungus) (Fusarium graminearum)

Taxonomic identifier

229533 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Hypocreomycetidae › Hypocreales › Nectriaceae › Gibberella

Protein attributes

Sequence length	564 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Adds a myristoyl group to the N-terminal glycine residue of certain cellular proteins By similarity.
Catalytic activity	Tetradecanoyl-CoA + glycylpeptide = CoA + N-tetradecanoylglycylpeptide.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the NMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	N-terminal protein myristoylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycylpeptide N-tetradecanoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 564	564	Glycylpeptide N-tetradecanoyltransferase		PRO_0000064242
Regions
	Region	153 – 156	4	Myristoyl CoA-binding By similarity
	Region	283 – 319	37	Myristoyl CoA-binding By similarity
	Compositional bias	55 – 64	10	Poly-Lys
Sites
	Active site	564	1	Proton acceptor; via carboxylate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4I061 [UniParc]. Last modified August 16, 2005. Version 1. Checksum: CB68E90A8E477D6B	FASTA	564	63,944
	10 20 30 40 50 60 MSEESKPVEP VVDETNAELK GKAIAQADPE ADEHAESASE EEHEEEHAAE GSSDKKKKKK 70 80 90 100 110 120 KKSKRSKVKE ALTGSKSAPT DADFHKALDG LTPKEVKEFL ALNPALAQEV SKASNSDNPS 130 140 150 160 170 180 ADQASDMLKK MSLQDIMTGL AAGGKNAKDM GSYKFWQTQP VPKFGEDNNL QEGPLRIQKV 190 200 210 220 230 240 EEVDKEPSAL IPGFEWVTMD LTKEEEIKEV YELLNKHYVE DDEAMFRFNY SPTILRWAMM 250 260 270 280 290 300 PPGWKKEYHV GVRASQSGLL CAFISAIPVH LRVRDNVVTC SEVNFLAIHK KLRGKRLTPV 310 320 330 340 350 360 LIKEITRRSN LDGIWQGLYT AGVVLPKPVS TCRYFHRAIN WQKLYECGFS PLPANSKPQY 370 380 390 400 410 420 QIRKYALPDD TAIKGLRPLQ EKDIEAVMDL YHRYNKRFDM TPKMSREEAL HWFLPKTGPG 430 440 450 460 470 480 EQQAVWTYVV EDENNKITDF FSFFCIESTA IGNSKHNVIK VAYMFYYGTE VGLQDKFDKA 490 500 510 520 530 540 ALKKRLNDLV HDALIISKRY KFDVFNALTL MDNALFLEQQ KFGAGDGQLH YYLFNYRVNP 550 560 IAGGVDRKNQ LDEENLSGIG LVMP

References

[1]

"The Fusarium graminearum genome reveals a link between localized polymorphism and pathogen specialization." Cuomo C.A., Gueldener U., Xu J.-R., Trail F., Turgeon B.G., Di Pietro A., Walton J.D., Ma L.-J., Baker S.E., Rep M., Adam G., Antoniw J., Baldwin T., Calvo S.E., Chang Y.-L., DeCaprio D., Gale L.R., Gnerre S. , Goswami R.S., Hammond-Kosack K., Harris L.J., Hilburn K., Kennell J.C., Kroken S., Magnuson J.K., Mannhaupt G., Mauceli E.W., Mewes H.-W., Mitterbauer R., Muehlbauer G., Muensterkoetter M., Nelson D., O'Donnell K., Ouellet T., Qi W., Quesneville H., Roncero M.I.G., Seong K.-Y., Tetko I.V., Urban M., Waalwijk C., Ward T.J., Yao J., Birren B.W., Kistler H.C. Science 317:1400-1402(2007) [PubMed: 17823352] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PH-1 / ATCC MYA-4620 / FGSC 9075 / NRRL 31084.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACM01000384 Genomic DNA. Translation: EAA77389.1.
RefSeq	XP_389573.1. XM_389573.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	FGSG_09397T0; FGSG_09397P0; FGSG_09397.
GeneID	2791587.
KEGG	fgr:FG09397.1.
Family and domain databases
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000903. MyristoylCoA_TrFase. IPR022677. MyristoylCoA_TrFase_C. IPR022678. MyristoylCoA_TrFase_CS. IPR022676. MyristoylCoA_TrFase_N. [Graphical view]
Gene3D	G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 2 hits.
KO	K00671.
PANTHER	PTHR11377. Myristoyl_trans. 1 hit.
Pfam	PF01233. NMT. 1 hit. PF02799. NMT_C. 1 hit. [Graphical view]
PIRSF	PIRSF015892. N-myristl_transf. 1 hit.
SUPFAM	SSF55729. Acyl_CoA_acyltransferase. 2 hits.
PROSITE	PS00975. NMT_1. False negative. PS00976. NMT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NMT_GIBZE

Accession

Primary (citable) accession number: Q4I061

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	August 16, 2005
Last modified:	December 14, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program