Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isocitrate lyase

Gene

ICL1

Organism
Gibberella zeae (strain PH-1 / ATCC MYA-4620 / FGSC 9075 / NRRL 31084) (Wheat head blight fungus) (Fusarium graminearum)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle. Required for growth on ethanol or acetate, but dispensable when fermentable carbon sources are available. Acts also on 2-methylisocitrate.By similarity

Catalytic activityi

Isocitrate = succinate + glyoxylate.By similarity
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate.By similarity

Cofactori

Mg2+By similarity

Pathwayi: glyoxylate cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from isocitrate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Isocitrate lyase (ICL1)
  2. Malate synthase (FG08700.1)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi177MagnesiumBy similarity1
Active sitei215Proton acceptorBy similarity1
Binding sitei252SubstrateBy similarity1
Binding sitei466SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00719.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate lyaseBy similarity (EC:4.1.3.1By similarity)
Short name:
ICLCurated
Short name:
IsocitraseCurated
Short name:
IsocitrataseCurated
Alternative name(s):
Methylisocitrate lyaseBy similarity (EC:4.1.3.30By similarity)
Short name:
MICACurated
Threo-D(S)-isocitrate glyoxylate-lyaseCurated
Gene namesi
Name:ICL1By similarity
ORF Names:FGRRES_09896, FGSG_09896
OrganismiGibberella zeae (strain PH-1 / ATCC MYA-4620 / FGSC 9075 / NRRL 31084) (Wheat head blight fungus) (Fusarium graminearum)
Taxonomic identifieri229533 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesNectriaceaeFusarium
Proteomesi
  • UP000070720 Componenti: Chromosome 1
  • UP000009057 Componenti: Unplaced

Organism-specific databases

EuPathDBiFungiDB:FGSG_09896.

Subcellular locationi

  • Glyoxysome By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Glyoxysome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002861571 – 546Isocitrate lyaseAdd BLAST546

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi229533.XP_390072.1.

Structurei

Secondary structure

1546
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni13 – 16Combined sources4
Helixi17 – 31Combined sources15
Helixi34 – 36Combined sources3
Helixi45 – 49Combined sources5
Helixi60 – 77Combined sources18
Beta strandi82 – 86Combined sources5
Helixi90 – 96Combined sources7
Turni97 – 99Combined sources3
Beta strandi103 – 105Combined sources3
Helixi107 – 113Combined sources7
Beta strandi122 – 125Combined sources4
Helixi131 – 155Combined sources25
Helixi159 – 161Combined sources3
Beta strandi174 – 177Combined sources4
Beta strandi182 – 184Combined sources3
Helixi185 – 198Combined sources14
Beta strandi201 – 208Combined sources8
Turni210 – 212Combined sources3
Helixi226 – 243Combined sources18
Beta strandi248 – 253Combined sources6
Turni255 – 257Combined sources3
Beta strandi260 – 262Combined sources3
Helixi267 – 270Combined sources4
Helixi284 – 293Combined sources10
Helixi298 – 311Combined sources14
Helixi317 – 327Combined sources11
Helixi333 – 344Combined sources12
Helixi349 – 360Combined sources12
Beta strandi367 – 369Combined sources3
Beta strandi378 – 380Combined sources3
Helixi384 – 394Combined sources11
Helixi395 – 397Combined sources3
Beta strandi399 – 403Combined sources5
Helixi410 – 421Combined sources12
Beta strandi429 – 432Combined sources4
Helixi439 – 442Combined sources4
Helixi445 – 448Combined sources4
Helixi451 – 457Combined sources7
Beta strandi460 – 465Combined sources6
Helixi468 – 487Combined sources20
Helixi489 – 495Combined sources7
Helixi497 – 502Combined sources6
Helixi506 – 508Combined sources3
Helixi510 – 513Combined sources4
Helixi516 – 525Combined sources10
Helixi541 – 544Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5E9HX-ray2.30A/B1-546[»]
ProteinModelPortaliQ4HYR2.
SMRiQ4HYR2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni106 – 108Substrate bindingBy similarity3
Regioni216 – 217Substrate bindingBy similarity2
Regioni432 – 436Substrate bindingBy similarity5

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1260. Eukaryota.
COG2224. LUCA.
InParanoidiQ4HYR2.
KOiK01637.
OrthoDBiEOG092C19UX.

Family and domain databases

Gene3Di3.20.20.60. 2 hits.
InterProiIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF00463. ICL. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR01346. isocit_lyase. 1 hit.
PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4HYR2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASQNMTNPS INPDIEDELF QKEVEAVKTW WSDSRWRQTK RPFTAEQIVS
60 70 80 90 100
KRGYLPIDYA SNTQAKKLWK ILEHRFENRD ASYTYGCLEP TMVTQMAKYL
110 120 130 140 150
DTVYVSGWQS SSTASASDEP GPDLADYPYT TVPNKVGHLF MAQLFHDRKQ
160 170 180 190 200
RQERLSVPKE QRANLLNIDY LRPIVADADT GHGGLTAVMK LTKLFIEKGA
210 220 230 240 250
AGIHIEDQAP GTKKCGHMAG KVLVPIQEHI NRLVAIRAQA DIMGSDLLAI
260 270 280 290 300
ARTDAEAATL LSTNIDPRDH AFILGSTNST LKPLNDLMIA AEATGKSGAE
310 320 330 340 350
LQRIEDEWLA KANLSSFDDA VAAAIDAGSF SDKAGIKQEY TSRAKGKSNF
360 370 380 390 400
EARAVARQLL GRDIFFDWDA PRTREGYFRL KGGCDCAVNR AIAYAPYCDA
410 420 430 440 450
IWMESKLPDF AQAEQFAQGV HAVWPEKKLA YNLSPSFNWK TAMPRDEQET
460 470 480 490 500
YIRRLAKLGY CWQFITLAGL HTTALISDQF AKAYSTVGMR AYGELVQEPE
510 520 530 540
MDQKVDVVKH QKWSGATYVD ELQKMVTGGI SSTAAMGAGV TEDQFK
Length:546
Mass (Da):60,826
Last modified:August 16, 2005 - v1
Checksum:iC90BD9BFBAB4BE9D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS231669 Genomic DNA. Translation: ESU16540.1.
HG970332 Genomic DNA. Translation: CEF75197.1.
RefSeqiXP_011318802.1. XM_011320500.1.

Genome annotation databases

GeneIDi23556822.
KEGGifgr:FGSG_09896.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS231669 Genomic DNA. Translation: ESU16540.1.
HG970332 Genomic DNA. Translation: CEF75197.1.
RefSeqiXP_011318802.1. XM_011320500.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5E9HX-ray2.30A/B1-546[»]
ProteinModelPortaliQ4HYR2.
SMRiQ4HYR2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi229533.XP_390072.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi23556822.
KEGGifgr:FGSG_09896.

Organism-specific databases

EuPathDBiFungiDB:FGSG_09896.

Phylogenomic databases

eggNOGiKOG1260. Eukaryota.
COG2224. LUCA.
InParanoidiQ4HYR2.
KOiK01637.
OrthoDBiEOG092C19UX.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00719.

Family and domain databases

Gene3Di3.20.20.60. 2 hits.
InterProiIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF00463. ICL. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR01346. isocit_lyase. 1 hit.
PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACEA_GIBZE
AccessioniPrimary (citable) accession number: Q4HYR2
Secondary accession number(s): A0A0E0RVC2, V6RX12
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: August 16, 2005
Last modified: November 30, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.