Reviewed, UniProtKB/Swiss-Prot Q4H1G4 (METK1_BETVU)

Names and origin

Protein names

Recommended name:
    S-adenosylmethionine synthetase 1
      Short name=AdoMet synthetase 1
    EC=2.5.1.6
Alternative name(s):
    Methionine adenosyltransferase 1
      Short name=MAT 1

Gene names

Name:

SAMS1

Organism

Beta vulgaris (Sugar beet)

Taxonomic identifier

161934 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Amaranthaceae › Beta

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme By similarity. May be involved in the synthesis of betain in response to abiotic stress such as high salinity.
Catalytic activity	ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.
Cofactor	Binds 2 divalent ions per subunit. Magnesium or cobalt By similarity. Binds 1 potassium ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Induction	By salt stress. Follow a circadian regulation with higher levels in the light. Ref.1
Sequence similarities	Belongs to the AdoMet synthetase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Cobalt Magnesium Metal-binding Nucleotide-binding Potassium
Molecular function	Transferase
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW methionine adenosyltransferase activity Inferred from electronic annotation. Source: EC potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 396	396	S-adenosylmethionine synthetase 1		PRO_0000363009
Regions
	Nucleotide binding	123 – 128	6	ATP Potential
	Nucleotide binding	271 – 278	8	ATP Potential
Sites
	Metal binding	21	1	Magnesium By similarity
	Metal binding	47	1	Potassium By similarity
	Metal binding	275	1	Potassium By similarity
	Metal binding	283	1	Magnesium By similarity
	Binding site	151	1	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q4H1G4-1 [UniParc]. Last modified August 30, 2005. Version 1. Checksum: 15A9739C1E74DE1D	FASTA	396	43,216
	10 20 30 40 50 60 MAAPIDTFLF TSESVNEGHP DKMCDQISDA VLDACLAQDP ESKVACETCT KTNLVMVFGE 70 80 90 100 110 120 ITTKGNVDYE KIVRQTCRDI GFVSADVGLD ADNCKVLVYI EQQSPDIAQG VHGHLTKRPE 130 140 150 160 170 180 EIGAGDQGHM FGYATDETPE LMPLSHVLAT KLGARLTEVR KNGTCPWLRP DGKTQVTVEY 190 200 210 220 230 240 YNDNGAMVPI RVHTVLISTQ HDETVTNDEI AADLKEHVIK PVIPEKYLDE KTIFHLNPSG 250 260 270 280 290 300 RFVIGGPHGD AGLTGRKIII DTYGGWGAHG GGAFSGKDPT KVDRSGAYIA RQAAKSIVAS 310 320 330 340 350 360 GLARRCIVQI SYAIGVPEPL SVFVDTYGTG KIPDKDILKI VKETFDFRPG MIAINLDLLK 370 380 390 GGSRYLKTAA YGHFGRDDPD FTWEVVKPLK WDKPQA

References

[1]	"Transcriptional response of glycinebetaine-related genes to salt stress and light in leaf beet." Tabuchi T., Okada T., Takashima Y., Azuma T., Nanmori T., Yasuda T. Plant Biotechnol. 23:317-320(2006) Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB221009 mRNA. Translation: BAE07179.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR002133. S-AdoMet_synthetase. [Graphical view]
PANTHER	PTHR11964. S-AdoMet_synt. 1 hit.
Pfam	PF02773. S-AdoMet_synt_C. 1 hit. PF02772. S-AdoMet_synt_M. 1 hit. PF00438. S-AdoMet_synt_N. 1 hit. [Graphical view]
PIRSF	PIRSF000497. MAT. 1 hit.
TIGRFAMs	TIGR01034. metK. 1 hit.
PROSITE	PS00376. ADOMET_SYNTHETASE_1. 1 hit. PS00377. ADOMET_SYNTHETASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

METK1_BETVU

Accession

Primary (citable) accession number: Q4H1G4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	August 30, 2005
Last modified:	September 22, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)