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Protein

40S ribosomal protein SA

Gene

RPSA

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Also acts as a receptor for several other ligands, including the pathogenic prion protein, viruses, and bacteria. Acts as a PPP1R16B-dependent substrate of PPP1CA.UniRule annotation1 Publication

Miscellaneous

This protein appears to have acquired a second function as a laminin receptor specifically in the vertebrate lineage.
It is thought that in vertebrates 37/67 kDa laminin receptor acquired a dual function during evolution. It developed from the ribosomal protein SA, playing an essential role in the protein biosynthesis lacking any laminin binding activity, to a cell surface receptor with laminin binding activity.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHost cell receptor for virus entry, Receptor, Ribonucleoprotein, Ribosomal protein

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein SAUniRule annotation
Alternative name(s):
37 kDa laminin receptor precursorUniRule annotation
Short name:
37LRPUniRule annotation
37/67 kDa laminin receptorUniRule annotation
Short name:
LRP/LRUniRule annotation
67 kDa laminin receptorUniRule annotation
Short name:
67LRUniRule annotation
Laminin receptor 1UniRule annotation
Short name:
LamRUniRule annotation
Laminin-binding protein precursor p40UniRule annotation
Short name:
LBP/p40UniRule annotation
Gene namesi
Name:RPSAUniRule annotation
Synonyms:LAMR1
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedUniRule annotation
ChainiPRO_00002717512 – 29540S ribosomal protein SAAdd BLAST294

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineUniRule annotationBy similarity1
Modified residuei43PhosphoserineBy similarity1
Modified residuei52N6-acetyllysineBy similarity1
Modified residuei89N6-acetyllysine; alternateBy similarity1
Cross-linki89Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei97PhosphothreonineBy similarity1

Post-translational modificationi

Acylated. Acylation may be a prerequisite for conversion of the monomeric 37 kDa laminin receptor precursor (37LRP) to the mature dimeric 67 kDa laminin receptor (67LR), and may provide a mechanism for membrane association.UniRule annotation
Cleaved by stromelysin-3 (ST3) at the cell surface. Cleavage by stromelysin-3 may be a mechanism to alter cell-extracellular matrix interactions.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei115 – 116Cleavage; by ST3; site 1UniRule annotation2
Sitei133 – 134Cleavage; by ST3; site 2UniRule annotation2

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ4GWZ2.
PeptideAtlasiQ4GWZ2.
PRIDEiQ4GWZ2.

Interactioni

Subunit structurei

Monomer (37LRP) and homodimer (67LR). Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 33 different proteins and 1 molecule of RNA (18S). The 60S subunit contains about 49 different proteins and 3 molecules of RNA (28S, 5.8S and 5S). Interacts with RPS21. Interacts with several laminins including at least LAMB1. Interacts with MDK. The mature dimeric form interacts with PPP1R16B (via its fourth ankyrin repeat). Interacts with PPP1CA only in the presence of PPP1R16B.UniRule annotation

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000012012.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J7Pelectron microscopy3.50SA1-295[»]
3J7Relectron microscopy3.90SA1-295[»]
ProteinModelPortaliQ4GWZ2.
SMRiQ4GWZ2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati230 – 232[DE]-W-[ST] 13
Repeati247 – 249[DE]-W-[ST] 23
Repeati266 – 268[DE]-W-[ST] 33
Repeati275 – 277[DE]-W-[ST] 43
Repeati293 – 295[DE]-W-[ST] 53

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni54 – 113Interaction with PPP1R16BUniRule annotationAdd BLAST60
Regioni161 – 180Laminin-bindingUniRule annotationAdd BLAST20
Regioni205 – 229Laminin-bindingUniRule annotationAdd BLAST25
Regioni242 – 295Laminin-bindingUniRule annotationAdd BLAST54

Sequence similaritiesi

Belongs to the universal ribosomal protein uS2 family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0830. Eukaryota.
COG0052. LUCA.
HOGENOMiHOG000232073.
HOVERGENiHBG054466.
InParanoidiQ4GWZ2.
KOiK02998.

Family and domain databases

CDDicd01425. RPS2. 1 hit.
HAMAPiMF_03015. Ribosomal_S2_euk. 1 hit.
MF_03016. Ribosomal_S2_laminin_receptor. 1 hit.
InterProiView protein in InterPro
IPR027504. 40S_ribosomal_SA.
IPR032281. 40S_SA_C.
IPR001865. Ribosomal_S2.
IPR018130. Ribosomal_S2_CS.
IPR027498. Ribosomal_S2_euk.
IPR005707. Ribosomal_S2_euk/arc.
IPR023591. Ribosomal_S2_flav_dom.
PANTHERiPTHR11489. PTHR11489. 1 hit.
PfamiView protein in Pfam
PF16122. 40S_SA_C. 1 hit.
PF00318. Ribosomal_S2. 2 hits.
PRINTSiPR00395. RIBOSOMALS2.
SUPFAMiSSF52313. SSF52313. 1 hit.
TIGRFAMsiTIGR01012. uS2_euk_arch. 1 hit.
PROSITEiView protein in PROSITE
PS00962. RIBOSOMAL_S2_1. 1 hit.
PS00963. RIBOSOMAL_S2_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4GWZ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGALDVLQM KEEDVLKFLA AGTHLGGTNL DFQMEQYIYK RKSDGIYIIN
60 70 80 90 100
LKRTWEKLLL AARAIVAIEN PADVSVISSR NTGQRAVLKF AAATGATPIA
110 120 130 140 150
GRFTPGTFTN QIQAAFREPR LLVVTDPRAD HQPLTEASYV NLPTIALCNT
160 170 180 190 200
DSPLRYVDIA IPCNNKGAHS VGLMWWMLAR EVLRMRGTIS REHPWEVMPD
210 220 230 240 250
LYFYRDPEEI EKEEQAAAEK AVTKEEFQGE WTAPAPEFTA TQPEVADWSE
260 270 280 290
GVQVPSVPIQ QFPTEDWSAQ PTTEDWSAAP TAQATEWVGT TTEWS
Length:295
Mass (Da):32,928
Last modified:January 23, 2007 - v3
Checksum:iC7C8DAA6B7459785
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM050292 mRNA. Translation: CAJ18354.1.
RefSeqiNP_001032223.1. NM_001037146.2.
UniGeneiSsc.11095.
Ssc.66959.

Genome annotation databases

GeneIDi641351.
KEGGissc:641351.

Similar proteinsi

Entry informationi

Entry nameiRSSA_PIG
AccessioniPrimary (citable) accession number: Q4GWZ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: January 23, 2007
Last modified: October 25, 2017
This is version 86 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families