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Q4GWU5

- SFTI1_HELAN

UniProt

Q4GWU5 - SFTI1_HELAN

Protein

Trypsin inhibitor 1

Gene

sfti1

Organism
Helianthus annuus (Common sunflower)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Inhibits trypsin, cathepsin G, elastase, chymotrypsin and thrombin. Does not inhibit factor Xa.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei44 – 452Reactive bondBy similarity

    GO - Molecular functioni

    1. serine-type endopeptidase inhibitor activity Source: UniProtKB

    GO - Biological processi

    1. negative regulation of endopeptidase activity Source: GOC

    Keywords - Molecular functioni

    Protease inhibitor, Serine protease inhibitor

    Protein family/group databases

    MEROPSiI12.002.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trypsin inhibitor 1
    Alternative name(s):
    SFTI-1
    Gene namesi
    Name:sfti1Imported
    OrganismiHelianthus annuus (Common sunflower)
    Taxonomic identifieri4232 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridscampanulidsAsteralesAsteraceaeAsteroideaeHeliantheae allianceHeliantheaeHelianthus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525Sequence AnalysisAdd
    BLAST
    Propeptidei26 – 39141 PublicationSequence AnalysisPRO_0000042672Add
    BLAST
    Peptidei40 – 5314Trypsin inhibitor 1PRO_0000042673Add
    BLAST
    Propeptidei54 – 563PRO_0000042674

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki40 ↔ 53Cyclopeptide (Gly-Asp)
    Disulfide bondi42 ↔ 502 Publications

    Post-translational modificationi

    This is a cyclic peptide.1 Publication

    Keywords - PTMi

    Disulfide bond

    Structurei

    Secondary structure

    1
    56
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi34 – 363
    Beta strandi41 – 477
    Beta strandi49 – 524

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1JBLNMR-A40-53[»]
    1JBNNMR-A40-53[»]
    1O8YNMR-A40-53[»]
    1O8ZNMR-A40-53[»]
    1SFIX-ray1.65I40-53[»]
    1T9ENMR-A40-53[»]
    2AB9NMR-A26-56[»]
    3P8FX-ray2.00I40-53[»]
    4ABIX-ray1.55B40-53[»]
    4ABJX-ray1.45B40-53[»]
    4HGCX-ray1.29I40-53[»]
    4K1EX-ray1.30B40-53[»]
    4K8YX-ray1.00B40-53[»]
    4KELX-ray1.15B40-53[»]
    ProteinModelPortaliQ4GWU5.
    SMRiQ4GWU5. Positions 26-56.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ4GWU5.

    Family & Domainsi

    Keywords - Domaini

    Signal

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q4GWU5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATTMAKLIT LVVLAILAFV EVSVSGYKTS ISTITIEDNG RCTKSIPPIC   50
    FPDGRP 56
    Length:56
    Mass (Da):5,971
    Last modified:August 30, 2005 - v1
    Checksum:iBF3BAF97C25F19A7
    GO

    Mass spectrometryi

    Molecular mass is 1513 Da from positions 40 - 53. Determined by ESI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM050587 Genomic DNA. Translation: CAJ19097.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM050587 Genomic DNA. Translation: CAJ19097.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1JBL NMR - A 40-53 [» ]
    1JBN NMR - A 40-53 [» ]
    1O8Y NMR - A 40-53 [» ]
    1O8Z NMR - A 40-53 [» ]
    1SFI X-ray 1.65 I 40-53 [» ]
    1T9E NMR - A 40-53 [» ]
    2AB9 NMR - A 26-56 [» ]
    3P8F X-ray 2.00 I 40-53 [» ]
    4ABI X-ray 1.55 B 40-53 [» ]
    4ABJ X-ray 1.45 B 40-53 [» ]
    4HGC X-ray 1.29 I 40-53 [» ]
    4K1E X-ray 1.30 B 40-53 [» ]
    4K8Y X-ray 1.00 B 40-53 [» ]
    4KEL X-ray 1.15 B 40-53 [» ]
    ProteinModelPortali Q4GWU5.
    SMRi Q4GWU5. Positions 26-56.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi I12.002.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei Q4GWU5.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Discovery, structural determination, and putative processing of the precursor protein that produces the cyclic trypsin inhibitor sunflower trypsin inhibitor 1."
      Mulvenna J.P., Foley F.M., Craik D.J.
      J. Biol. Chem. 280:32245-32253(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRUCTURE BY NMR OF 26-56.
      Tissue: LeafImported.
    2. "High-resolution structure of a potent, cyclic proteinase inhibitor from sunflower seeds."
      Luckett S., Garcia R.S., Barker J.J., Konarev A.V., Shewry P.R., Clarke A.R., Brady R.L.
      J. Mol. Biol. 290:525-533(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 40-53, CROSS-LINK, FUNCTION, MASS SPECTROMETRY, STRUCTURE BY NMR OF 40-53.
      Tissue: Seed1 Publication.
    3. "Solution structures by 1H NMR of the novel cyclic trypsin inhibitor SFTI-1 from sunflower seeds and an acyclic permutant."
      Korsinczky M.L., Schirra H.J., Rosengren K.J., West J., Condie B.A., Otvos L., Anderson M.A., Craik D.J.
      J. Mol. Biol. 311:579-591(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 40-53.
    4. "Enzymatic cyclization of a potent bowman-birk protease inhibitor, sunflower trypsin inhibitor-1, and solution structure of an acyclic precursor peptide."
      Marx U.C., Korsinczky M.L.J., Schirra H.J., Jones A., Condie B., Otvos L. Jr., Craik D.J.
      J. Biol. Chem. 278:21782-21789(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 40-53, DISULFIDE BOND.

    Entry informationi

    Entry nameiSFTI1_HELAN
    AccessioniPrimary (citable) accession number: Q4GWU5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 25, 2005
    Last sequence update: August 30, 2005
    Last modified: October 1, 2014
    This is version 35 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3