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Q4GWU5 (SFTI1_HELAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 34. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Trypsin inhibitor 1
Alternative name(s):
SFTI-1
Gene names
Name:sfti1
OrganismHelianthus annuus (Common sunflower)
Taxonomic identifier4232 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridscampanulidsAsteralesAsteraceaeAsteroideaeHeliantheae allianceHeliantheaeHelianthus

Protein attributes

Sequence length56 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibits trypsin, cathepsin G, elastase, chymotrypsin and thrombin. Does not inhibit factor Xa. Ref.2

Post-translational modification

This is a cyclic peptide. Ref.2

Mass spectrometry

Molecular mass is 1513 Da from positions 40 - 53. Determined by ESI. Ref.2

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Propeptide26 – 3914 Ref.2
PRO_0000042672
Peptide40 – 5314Trypsin inhibitor 1 Ref.2
PRO_0000042673
Propeptide54 – 563
PRO_0000042674

Sites

Site44 – 452Reactive bond By similarity UniProtKB P81705

Amino acid modifications

Disulfide bond42 ↔ 50 Ref.2 Ref.4
Cross-link40 ↔ 53Cyclopeptide (Gly-Asp)

Secondary structure

....... 56
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q4GWU5 [UniParc].

Last modified August 30, 2005. Version 1.
Checksum: BF3BAF97C25F19A7

FASTA565,971
        10         20         30         40         50 
MATTMAKLIT LVVLAILAFV EVSVSGYKTS ISTITIEDNG RCTKSIPPIC FPDGRP 

« Hide

References

[1]"Discovery, structural determination, and putative processing of the precursor protein that produces the cyclic trypsin inhibitor sunflower trypsin inhibitor 1."
Mulvenna J.P., Foley F.M., Craik D.J.
J. Biol. Chem. 280:32245-32253(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRUCTURE BY NMR OF 26-56.
Tissue: Leaf.
[2]"High-resolution structure of a potent, cyclic proteinase inhibitor from sunflower seeds."
Luckett S., Garcia R.S., Barker J.J., Konarev A.V., Shewry P.R., Clarke A.R., Brady R.L.
J. Mol. Biol. 290:525-533(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 40-53, CROSS-LINK, FUNCTION, MASS SPECTROMETRY, STRUCTURE BY NMR OF 40-53.
Tissue: Seed.
[3]"Solution structures by 1H NMR of the novel cyclic trypsin inhibitor SFTI-1 from sunflower seeds and an acyclic permutant."
Korsinczky M.L., Schirra H.J., Rosengren K.J., West J., Condie B.A., Otvos L., Anderson M.A., Craik D.J.
J. Mol. Biol. 311:579-591(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 40-53.
[4]"Enzymatic cyclization of a potent bowman-birk protease inhibitor, sunflower trypsin inhibitor-1, and solution structure of an acyclic precursor peptide."
Marx U.C., Korsinczky M.L.J., Schirra H.J., Jones A., Condie B., Otvos L. Jr., Craik D.J.
J. Biol. Chem. 278:21782-21789(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 40-53, DISULFIDE BOND.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM050587 Genomic DNA. Translation: CAJ19097.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1JBLNMR-A40-53[»]
1JBNNMR-A40-53[»]
1O8YNMR-A40-53[»]
1O8ZNMR-A40-53[»]
1SFIX-ray1.65I40-53[»]
1T9ENMR-A40-53[»]
2AB9NMR-A26-56[»]
3P8FX-ray2.00I40-53[»]
4ABIX-ray1.55B40-53[»]
4ABJX-ray1.45B40-53[»]
4HGCX-ray1.29I40-53[»]
4K1EX-ray1.30B40-53[»]
4K8YX-ray1.00B40-53[»]
4KELX-ray1.15B40-53[»]
ProteinModelPortalQ4GWU5.
SMRQ4GWU5. Positions 26-56.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSI12.002.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Other

EvolutionaryTraceQ4GWU5.

Entry information

Entry nameSFTI1_HELAN
AccessionPrimary (citable) accession number: Q4GWU5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: August 30, 2005
Last modified: July 9, 2014
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references