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Protein

Inhibitor of nuclear factor kappa-B kinase subunit alpha

Gene

chuk

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.By similarity

Catalytic activityi

ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein].

Enzyme regulationi

Activated when phosphorylated and inactivated when dephosphorylated.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei44 – 441ATPPROSITE-ProRule annotation
Active sitei145 – 1451Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi21 – 299ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • cellular response to tumor necrosis factor Source: UniProtKB
  • central nervous system development Source: ZFIN
  • epiboly involved in gastrulation with mouth forming second Source: ZFIN
  • I-kappaB kinase/NF-kappaB signaling Source: ZFIN
  • negative regulation of apoptotic process Source: ZFIN
  • negative regulation of I-kappaB kinase/NF-kappaB signaling Source: ZFIN
  • positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • somite specification Source: ZFIN
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Inhibitor of nuclear factor kappa-B kinase subunit alpha (EC:2.7.11.10)
Short name:
I kappa-B kinase alpha
Short name:
IKK-A
Short name:
IKK-alpha
Short name:
IkBKA
Short name:
IkappaB kinase
Alternative name(s):
Conserved helix-loop-helix ubiquitous kinase
I-kappa-B kinase 1
Short name:
IKK1
Nuclear factor NF-kappa-B inhibitor kinase alpha
Short name:
NFKBIKA
Gene namesi
Name:chuk
Synonyms:ikk1
ORF Names:zgc:56539
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-040426-1069. chuk.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Shuttles between the cytoplasm and the nucleus.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 758758Inhibitor of nuclear factor kappa-B kinase subunit alphaPRO_0000268827Add
BLAST

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ4G3H4.

Expressioni

Developmental stagei

Abundantly expressed from the blastula period through early gastrulation. During early somitogenesis, transcripts are dorsally localized at the level of the segmental plate and the presumptive notochord. At the 18-somite stage, expression becomes largely detectable throughout the somitic tissue At late embryonic stages, expression is particularly evident in the tectum, cerebellum, hindbrain region, and eyes. After hatching, transcripts can also be detected in the myotomes and pectoral fins.1 Publication

Interactioni

Subunit structurei

Directly interacts with ikbkg/nemo.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000013115.

Structurei

3D structure databases

ProteinModelPortaliQ4G3H4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini15 – 301287Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni456 – 47722Leucine-zipperAdd
BLAST
Regioni741 – 7466NEMO-bindingBy similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4250. Eukaryota.
ENOG410XRMU. LUCA.
HOGENOMiHOG000038048.
HOVERGENiHBG018241.
InParanoidiQ4G3H4.
KOiK04467.
PhylomeDBiQ4G3H4.

Family and domain databases

InterProiIPR022007. IKKbetaNEMObind.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF12179. IKKbetaNEMObind. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM01239. IKKbetaNEMObind. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4G3H4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKPPFRQNQ VCGSWVMKER LGTGGFGHVY LYQNQETSEK IAVKLCRLEL
60 70 80 90 100
NSKNKDRWSR GIQIMKKLKH LNVVTAKDVP EEMMHIALND LPLLAMEYCS
110 120 130 140 150
KGDLRKLLSK PENCCGLKES EVLSLLNDVG SGIQYLHENK IIHRDLKPEN
160 170 180 190 200
IVLQEINGKL VHKIIDLGYA KDLDQGSLCT SFVGTLQYLA PELFEGKSYT
210 220 230 240 250
VTVDFWSFGT MIFECCCGFR PFLHNLQPVQ WTSKVRNKGP KDIMAVEDMN
260 270 280 290 300
GEVRFSTHLP YPNNLSRTLL EPLEGLLQLM LKWDPVQRGL GLNTDSKQPQ
310 320 330 340 350
CFVLLDQILS MKVVHILNMT TTQVHSFLLS PDEGLHSLQQ RIENETKIEL
360 370 380 390 400
LNQDLLQETG VMSDPRKPAA QCVLDGVRGW DSYIVYLFDK SLTKYMGPLT
410 420 430 440 450
ARTLPESVNF IVRETKTQLP LSTLKKVWGE AVSYICGLRE DYSRLFQGQR
460 470 480 490 500
AAMLSLLRFN TNLTRYKNMM FSFSQQLKAK LDFFKTSIQY DLEKYSDQMQ
510 520 530 540 550
YGISSEKMLK AWHENEERAA AFAQVAEIGH LDEEIMALHS EIVELQRSPY
560 570 580 590 600
ARRQGDVMEQ LQEKAIELYK QLKAKCKMPD PQHGYSDSSE MVKVIVQTVQ
610 620 630 640 650
NQDRVLRDLY AHLSKILLSK QKIIDLFPKI ERTLECIKEA DTTVMQMQIK
660 670 680 690 700
RQREFWHLLK IACAQNTTRS SVSQSGEMPS SLSTWNQTQA QCSSRLPMSL
710 720 730 740 750
QVPHEGDSVN HLLEENQRYL TQLTSLLQET TEEKSESIMA QDWSWTKYES

LVAKSPRL
Length:758
Mass (Da):87,147
Last modified:August 30, 2005 - v1
Checksum:i9E3355AEAE5EE7F7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti61 – 611G → E in AAW68010 (PubMed:16051172).Curated
Sequence conflicti363 – 3631S → L in AAW68010 (PubMed:16051172).Curated
Sequence conflicti438 – 4381L → P in AAW68010 (PubMed:16051172).Curated
Sequence conflicti617 – 6171L → P in AAW68010 (PubMed:16051172).Curated
Sequence conflicti756 – 7561P → A in AAW68010 (PubMed:16051172).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY735397 mRNA. Translation: AAW68010.1.
BC051614 mRNA. Translation: AAH51614.1.
RefSeqiNP_956611.1. NM_200317.1.
UniGeneiDr.14012.

Genome annotation databases

GeneIDi393287.
KEGGidre:393287.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY735397 mRNA. Translation: AAW68010.1.
BC051614 mRNA. Translation: AAH51614.1.
RefSeqiNP_956611.1. NM_200317.1.
UniGeneiDr.14012.

3D structure databases

ProteinModelPortaliQ4G3H4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000013115.

Proteomic databases

PaxDbiQ4G3H4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi393287.
KEGGidre:393287.

Organism-specific databases

CTDi1147.
ZFINiZDB-GENE-040426-1069. chuk.

Phylogenomic databases

eggNOGiKOG4250. Eukaryota.
ENOG410XRMU. LUCA.
HOGENOMiHOG000038048.
HOVERGENiHBG018241.
InParanoidiQ4G3H4.
KOiK04467.
PhylomeDBiQ4G3H4.

Miscellaneous databases

PROiQ4G3H4.

Family and domain databases

InterProiIPR022007. IKKbetaNEMObind.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF12179. IKKbetaNEMObind. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM01239. IKKbetaNEMObind. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIKKA_DANRE
AccessioniPrimary (citable) accession number: Q4G3H4
Secondary accession number(s): Q7ZTU1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: August 30, 2005
Last modified: June 8, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.