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Q4G3H4 (IKKA_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Inhibitor of nuclear factor kappa-B kinase subunit alpha
Short name=I kappa-B kinase alpha
Short name=IKK-A
Short name=IKK-alpha
Short name=IkBKA
Short name=IkappaB kinase
EC=2.7.11.10
Alternative name(s):
Conserved helix-loop-helix ubiquitous kinase
I-kappa-B kinase 1
Short name=IKK1
Nuclear factor NF-kappa-B inhibitor kinase alpha
Short name=NFKBIKA
Gene names
Name:chuk
Synonyms:ikk1
ORF Names:zgc:56539
OrganismDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length758 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines By similarity.

Catalytic activity

ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein].

Enzyme regulation

Activated when phosphorylated and inactivated when dephosphorylated By similarity.

Subunit structure

Directly interacts with ikbkg/nemo. Ref.1

Subcellular location

Cytoplasm By similarity. Nucleus By similarity. Note: Shuttles between the cytoplasm and the nucleus By similarity.

Developmental stage

Abundantly expressed from the blastula period through early gastrulation. During early somitogenesis, transcripts are dorsally localized at the level of the segmental plate and the presumptive notochord. At the 18-somite stage, expression becomes largely detectable throughout the somitic tissue At late embryonic stages, expression is particularly evident in the tectum, cerebellum, hindbrain region, and eyes. After hatching, transcripts can also be detected in the myotomes and pectoral fins. Ref.1

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 758758Inhibitor of nuclear factor kappa-B kinase subunit alpha
PRO_0000268827

Regions

Domain15 – 301287Protein kinase
Domain456 – 47722Leucine-zipper
Nucleotide binding21 – 299ATP By similarity
Region741 – 7466NEMO-binding By similarity

Sites

Active site1451Proton acceptor By similarity
Binding site441ATP By similarity

Experimental info

Sequence conflict611G → E in AAW68010. Ref.1
Sequence conflict3631S → L in AAW68010. Ref.1
Sequence conflict4381L → P in AAW68010. Ref.1
Sequence conflict6171L → P in AAW68010. Ref.1
Sequence conflict7561P → A in AAW68010. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q4G3H4 [UniParc].

Last modified August 30, 2005. Version 1.
Checksum: 9E3355AEAE5EE7F7

FASTA75887,147
        10         20         30         40         50         60 
MEKPPFRQNQ VCGSWVMKER LGTGGFGHVY LYQNQETSEK IAVKLCRLEL NSKNKDRWSR 

        70         80         90        100        110        120 
GIQIMKKLKH LNVVTAKDVP EEMMHIALND LPLLAMEYCS KGDLRKLLSK PENCCGLKES 

       130        140        150        160        170        180 
EVLSLLNDVG SGIQYLHENK IIHRDLKPEN IVLQEINGKL VHKIIDLGYA KDLDQGSLCT 

       190        200        210        220        230        240 
SFVGTLQYLA PELFEGKSYT VTVDFWSFGT MIFECCCGFR PFLHNLQPVQ WTSKVRNKGP 

       250        260        270        280        290        300 
KDIMAVEDMN GEVRFSTHLP YPNNLSRTLL EPLEGLLQLM LKWDPVQRGL GLNTDSKQPQ 

       310        320        330        340        350        360 
CFVLLDQILS MKVVHILNMT TTQVHSFLLS PDEGLHSLQQ RIENETKIEL LNQDLLQETG 

       370        380        390        400        410        420 
VMSDPRKPAA QCVLDGVRGW DSYIVYLFDK SLTKYMGPLT ARTLPESVNF IVRETKTQLP 

       430        440        450        460        470        480 
LSTLKKVWGE AVSYICGLRE DYSRLFQGQR AAMLSLLRFN TNLTRYKNMM FSFSQQLKAK 

       490        500        510        520        530        540 
LDFFKTSIQY DLEKYSDQMQ YGISSEKMLK AWHENEERAA AFAQVAEIGH LDEEIMALHS 

       550        560        570        580        590        600 
EIVELQRSPY ARRQGDVMEQ LQEKAIELYK QLKAKCKMPD PQHGYSDSSE MVKVIVQTVQ 

       610        620        630        640        650        660 
NQDRVLRDLY AHLSKILLSK QKIIDLFPKI ERTLECIKEA DTTVMQMQIK RQREFWHLLK 

       670        680        690        700        710        720 
IACAQNTTRS SVSQSGEMPS SLSTWNQTQA QCSSRLPMSL QVPHEGDSVN HLLEENQRYL 

       730        740        750 
TQLTSLLQET TEEKSESIMA QDWSWTKYES LVAKSPRL

« Hide

References

« Hide 'large scale' references
[1]"Zebrafish IkappaB kinase 1 negatively regulates NF-kappaB activity."
Correa R.G., Matsui T., Tergaonkar V., Rodriguez-Esteban C., Izpisua-Belmonte J.C., Verma I.M.
Curr. Biol. 15:1291-1295(2005) [PubMed: 16051172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, INTERACTION WITH IKBKG.
[2]NIH - Zebrafish Gene Collection (ZGC) project
Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: SJD.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AY735397 mRNA. Translation: AAW68010.1.
BC051614 mRNA. Translation: AAH51614.1.
IPIIPI00485912.
RefSeqNP_956611.1. NM_200317.1.
UniGeneDr.14012.

3D structure databases

ProteinModelPortalQ4G3H4.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ4G3H4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID393287.
KEGGdre:393287.

Organism-specific databases

CTD1147.
ZFINZDB-GENE-040426-1069. chuk.

Phylogenomic databases

eggNOGfiNOG11989.
GeneTreeENSGT00530000063051.
HOGENOMHBG358635.
HOVERGENHBG018241.
InParanoidQ4G3H4.
OrthoDBEOG4SJ5D7.

Gene expression databases

ArrayExpressQ4G3H4.
BgeeQ4G3H4.

Family and domain databases

InterProIPR022007. IKKbetaNEMObind.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR017442. Se/Thr_kinase-like_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
KOK04467.
PfamPF12179. IKKbetaNEMObind. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMSSF56112. Kinase_like. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIKKA_DANRE
AccessionPrimary (citable) accession number: Q4G3H4
Secondary accession number(s): Q7ZTU1
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: August 30, 2005
Last modified: November 16, 2011
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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