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Protein

Inhibitor of nuclear factor kappa-B kinase subunit alpha

Gene

chuk

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.By similarity

Catalytic activityi

ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein].

Enzyme regulationi

Activated when phosphorylated and inactivated when dephosphorylated.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei44ATPPROSITE-ProRule annotation1
Active sitei145Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi21 – 29ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • cellular response to tumor necrosis factor Source: UniProtKB
  • central nervous system development Source: ZFIN
  • epiboly involved in gastrulation with mouth forming second Source: ZFIN
  • I-kappaB kinase/NF-kappaB signaling Source: ZFIN
  • I-kappaB phosphorylation Source: GOC
  • inflammatory response Source: GO_Central
  • innate immune response Source: GO_Central
  • negative regulation of apoptotic process Source: ZFIN
  • negative regulation of I-kappaB kinase/NF-kappaB signaling Source: ZFIN
  • peptidyl-serine phosphorylation Source: GO_Central
  • positive regulation of NF-kappaB transcription factor activity Source: GO_Central
  • positive regulation of transcription by RNA polymerase II Source: UniProtKB
  • somite specification Source: ZFIN
  • tumor necrosis factor-mediated signaling pathway Source: GO_Central

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Inhibitor of nuclear factor kappa-B kinase subunit alpha (EC:2.7.11.10)
Short name:
I kappa-B kinase alpha
Short name:
IKK-A
Short name:
IKK-alpha
Short name:
IkBKA
Short name:
IkappaB kinase
Alternative name(s):
Conserved helix-loop-helix ubiquitous kinase
I-kappa-B kinase 1
Short name:
IKK1
Nuclear factor NF-kappa-B inhibitor kinase alpha
Short name:
NFKBIKA
Gene namesi
Name:chuk
Synonyms:ikk1
ORF Names:zgc:56539
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-040426-1069 chuk

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002688271 – 758Inhibitor of nuclear factor kappa-B kinase subunit alphaAdd BLAST758

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ4G3H4
PRIDEiQ4G3H4

Expressioni

Developmental stagei

Abundantly expressed from the blastula period through early gastrulation. During early somitogenesis, transcripts are dorsally localized at the level of the segmental plate and the presumptive notochord. At the 18-somite stage, expression becomes largely detectable throughout the somitic tissue At late embryonic stages, expression is particularly evident in the tectum, cerebellum, hindbrain region, and eyes. After hatching, transcripts can also be detected in the myotomes and pectoral fins.1 Publication

Interactioni

Subunit structurei

Directly interacts with ikbkg/nemo.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000013115

Structurei

3D structure databases

ProteinModelPortaliQ4G3H4
SMRiQ4G3H4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 301Protein kinasePROSITE-ProRule annotationAdd BLAST287

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni456 – 477Leucine-zipperAdd BLAST22
Regioni741 – 746NEMO-bindingBy similarity6

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4250 Eukaryota
ENOG410XRMU LUCA
HOGENOMiHOG000038048
HOVERGENiHBG018241
InParanoidiQ4G3H4
KOiK04467
PhylomeDBiQ4G3H4

Family and domain databases

InterProiView protein in InterPro
IPR022007 IKKbetaNEMObind
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF12179 IKKbetaNEMObind, 1 hit
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM01239 IKKbetaNEMObind, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Q4G3H4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKPPFRQNQ VCGSWVMKER LGTGGFGHVY LYQNQETSEK IAVKLCRLEL
60 70 80 90 100
NSKNKDRWSR GIQIMKKLKH LNVVTAKDVP EEMMHIALND LPLLAMEYCS
110 120 130 140 150
KGDLRKLLSK PENCCGLKES EVLSLLNDVG SGIQYLHENK IIHRDLKPEN
160 170 180 190 200
IVLQEINGKL VHKIIDLGYA KDLDQGSLCT SFVGTLQYLA PELFEGKSYT
210 220 230 240 250
VTVDFWSFGT MIFECCCGFR PFLHNLQPVQ WTSKVRNKGP KDIMAVEDMN
260 270 280 290 300
GEVRFSTHLP YPNNLSRTLL EPLEGLLQLM LKWDPVQRGL GLNTDSKQPQ
310 320 330 340 350
CFVLLDQILS MKVVHILNMT TTQVHSFLLS PDEGLHSLQQ RIENETKIEL
360 370 380 390 400
LNQDLLQETG VMSDPRKPAA QCVLDGVRGW DSYIVYLFDK SLTKYMGPLT
410 420 430 440 450
ARTLPESVNF IVRETKTQLP LSTLKKVWGE AVSYICGLRE DYSRLFQGQR
460 470 480 490 500
AAMLSLLRFN TNLTRYKNMM FSFSQQLKAK LDFFKTSIQY DLEKYSDQMQ
510 520 530 540 550
YGISSEKMLK AWHENEERAA AFAQVAEIGH LDEEIMALHS EIVELQRSPY
560 570 580 590 600
ARRQGDVMEQ LQEKAIELYK QLKAKCKMPD PQHGYSDSSE MVKVIVQTVQ
610 620 630 640 650
NQDRVLRDLY AHLSKILLSK QKIIDLFPKI ERTLECIKEA DTTVMQMQIK
660 670 680 690 700
RQREFWHLLK IACAQNTTRS SVSQSGEMPS SLSTWNQTQA QCSSRLPMSL
710 720 730 740 750
QVPHEGDSVN HLLEENQRYL TQLTSLLQET TEEKSESIMA QDWSWTKYES

LVAKSPRL
Length:758
Mass (Da):87,147
Last modified:August 30, 2005 - v1
Checksum:i9E3355AEAE5EE7F7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti61G → E in AAW68010 (PubMed:16051172).Curated1
Sequence conflicti363S → L in AAW68010 (PubMed:16051172).Curated1
Sequence conflicti438L → P in AAW68010 (PubMed:16051172).Curated1
Sequence conflicti617L → P in AAW68010 (PubMed:16051172).Curated1
Sequence conflicti756P → A in AAW68010 (PubMed:16051172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY735397 mRNA Translation: AAW68010.1
BC051614 mRNA Translation: AAH51614.1
RefSeqiNP_956611.1, NM_200317.1
UniGeneiDr.14012

Genome annotation databases

GeneIDi393287
KEGGidre:393287

Similar proteinsi

Entry informationi

Entry nameiIKKA_DANRE
AccessioniPrimary (citable) accession number: Q4G3H4
Secondary accession number(s): Q7ZTU1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: August 30, 2005
Last modified: March 28, 2018
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health