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Q4G1G8 (CH25H_PIG) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Cholesterol 25-hydroxylase
EC=1.14.99.38
Alternative name(s):
Cholesterol 25-monooxygenase
Gene names
Name:CH25H
OrganismSus scrofa (Pig) [Reference proteome]
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length270 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation By similarity.

Catalytic activity

Cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O.

Cofactor

Iron By similarity.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Post-translational modification

N-glycosylated By similarity.

Sequence similarities

Belongs to the sterol desaturase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 270270Cholesterol 25-hydroxylase
PRO_0000226803

Regions

Transmembrane38 – 5821Helical; Potential
Transmembrane81 – 10323Helical; Potential
Transmembrane127 – 14721Helical; Potential
Transmembrane167 – 18721Helical; Potential
Transmembrane190 – 21021Helical; Potential
Motif142 – 1465Histidine box-1
Motif157 – 1615Histidine box-2
Motif238 – 2447Histidine box-3

Amino acid modifications

Glycosylation51N-linked (GlcNAc...) Potential
Glycosylation1631N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q4G1G8 [UniParc].

Last modified August 30, 2005. Version 1.
Checksum: 6BAEA05DBEAE1AA4

FASTA27031,482
        10         20         30         40         50         60 
MSGHNNSELF VLCSSSQLFL QPLWDHLKTW ETLILSPFFP VFFSITTYLG FCLPFVVLDV 

        70         80         90        100        110        120 
LCPWVPALRR YKIHPDFSPS VWQLLPCLGL TLYQHVVFVF PMTLLHWAAS PVLLPPEAPE 

       130        140        150        160        170        180 
LLQLVRHIVL CLLLFDTEFF IWHVLHHKVP WLYRTFHKMH HQNSSSFALA TQYMSVGELL 

       190        200        210        220        230        240 
SLGVFDMVNI MLLRCHPLTV LIFHVINIWL SVEDHSGYDF PWSAHRLVPF GWYGGVTHHD 

       250        260        270 
LHHSQFNCNF APYFTHWDKI LGTLRSAHAK

« Hide

References

[1]"Cloning and identification of a new single gene in pigs."
Cheng H.C., Deng C.Y., Zhang F.W.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AY974088 Genomic DNA. Translation: AAY40559.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRING9823.ENSSSCP00000011138.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG3000.
HOGENOMHOG000015767.
HOVERGENHBG080944.
OrthoDBEOG4N04FP.

Family and domain databases

InterProIPR006694. Fatty_acid_hydroxylase.
[Graphical view]
PfamPF04116. FA_hydroxylase. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCH25H_PIG
AccessionPrimary (citable) accession number: Q4G1G8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: August 30, 2005
Last modified: April 3, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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