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Q4G1G8

- CH25H_PIG

UniProt

Q4G1G8 - CH25H_PIG

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Protein
Cholesterol 25-hydroxylase
Gene
CH25H
Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation By similarity.

Catalytic activityi

Cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O.

Cofactori

Iron By similarity.

GO - Molecular functioni

  1. cholesterol 25-hydroxylase activity Source: UniProtKB-EC
  2. iron ion binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. fatty acid biosynthetic process Source: InterPro
  2. sterol biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cholesterol 25-hydroxylase (EC:1.14.99.38)
Alternative name(s):
Cholesterol 25-monooxygenase
Gene namesi
Name:CH25H
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei38 – 5821Helical; Reviewed prediction
Add
BLAST
Transmembranei81 – 10323Helical; Reviewed prediction
Add
BLAST
Transmembranei127 – 14721Helical; Reviewed prediction
Add
BLAST
Transmembranei167 – 18721Helical; Reviewed prediction
Add
BLAST
Transmembranei190 – 21021Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 270270Cholesterol 25-hydroxylase
PRO_0000226803Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi5 – 51N-linked (GlcNAc...) Reviewed prediction
Glycosylationi163 – 1631N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

N-glycosylated By similarity.

Keywords - PTMi

Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000011138.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi142 – 1465Histidine box-1
Motifi157 – 1615Histidine box-2
Motifi238 – 2447Histidine box-3

Sequence similaritiesi

Belongs to the sterol desaturase family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG3000.
HOGENOMiHOG000015767.
HOVERGENiHBG080944.

Family and domain databases

InterProiIPR006694. Fatty_acid_hydroxylase.
[Graphical view]
PfamiPF04116. FA_hydroxylase. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4G1G8-1 [UniParc]FASTAAdd to Basket

« Hide

MSGHNNSELF VLCSSSQLFL QPLWDHLKTW ETLILSPFFP VFFSITTYLG    50
FCLPFVVLDV LCPWVPALRR YKIHPDFSPS VWQLLPCLGL TLYQHVVFVF 100
PMTLLHWAAS PVLLPPEAPE LLQLVRHIVL CLLLFDTEFF IWHVLHHKVP 150
WLYRTFHKMH HQNSSSFALA TQYMSVGELL SLGVFDMVNI MLLRCHPLTV 200
LIFHVINIWL SVEDHSGYDF PWSAHRLVPF GWYGGVTHHD LHHSQFNCNF 250
APYFTHWDKI LGTLRSAHAK 270
Length:270
Mass (Da):31,482
Last modified:August 30, 2005 - v1
Checksum:i6BAEA05DBEAE1AA4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY974088 Genomic DNA. Translation: AAY40559.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY974088 Genomic DNA. Translation: AAY40559.1 .

3D structure databases

ModBasei Search...

Protein-protein interaction databases

STRINGi 9823.ENSSSCP00000011138.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG3000.
HOGENOMi HOG000015767.
HOVERGENi HBG080944.

Family and domain databases

InterProi IPR006694. Fatty_acid_hydroxylase.
[Graphical view ]
Pfami PF04116. FA_hydroxylase. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and identification of a new single gene in pigs."
    Cheng H.C., Deng C.Y., Zhang F.W.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiCH25H_PIG
AccessioniPrimary (citable) accession number: Q4G1G8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: August 30, 2005
Last modified: February 19, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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