Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

F-box only protein 43

Gene

FBXO43

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required to establish and maintain the arrest of oocytes at the second meiotic metaphase until fertilization. Probably acts by inhibiting the anaphase-promoting complex/cyclosome (APC/C) ubiquitin ligase. Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation (Probable).Curated

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri622 – 68160IBR-typeAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Meiosis, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
F-box only protein 43
Alternative name(s):
Endogenous meiotic inhibitor 2
Gene namesi
Name:FBXO43
Synonyms:EMI2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:28521. FBXO43.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi75 – 762DS → AA: Impairs ubiquitination and degradation in response to calcium. 1 Publication
Mutagenesisi333 – 3342DS → AA: Impairs ubiquitination and degradation in response to calcium. 1 Publication

Organism-specific databases

PharmGKBiPA134913061.

Polymorphism and mutation databases

BioMutaiFBXO43.
DMDMi152031603.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 708708F-box only protein 43PRO_0000247233Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei76 – 761Phosphoserine1 Publication
Modified residuei234 – 2341Phosphothreonine1 Publication
Modified residuei334 – 3341Phosphoserine1 Publication

Post-translational modificationi

Phosphorylated on Ser-76, Thr-234 and Ser-334 in response to calcium, which is a prerequisite for ubiquitination and proteasomal degradation.2 Publications
Ubiquitinated in response to calcium, which promotes proteasomal degradation.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ4G163.
PaxDbiQ4G163.
PRIDEiQ4G163.

PTM databases

iPTMnetiQ4G163.

Expressioni

Gene expression databases

BgeeiQ4G163.
CleanExiHS_FBXO43.
ExpressionAtlasiQ4G163. baseline and differential.
GenevisibleiQ4G163. HS.

Organism-specific databases

HPAiHPA024292.
HPA024295.
HPA024824.

Interactioni

Subunit structurei

Part of a SCF (SKP1-cullin-F-box) protein ligase complex. May interact with CDC20 (By similarity).By similarity

Protein-protein interaction databases

BioGridi130316. 2 interactions.
STRINGi9606.ENSP00000403293.

Structurei

3D structure databases

ProteinModelPortaliQ4G163.
SMRiQ4G163. Positions 633-684.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini490 – 54758F-boxAdd
BLAST

Sequence similaritiesi

Contains 1 F-box domain.Curated
Contains 1 IBR-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri622 – 68160IBR-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IHWX. Eukaryota.
ENOG410XUB4. LUCA.
GeneTreeiENSGT00530000063692.
HOGENOMiHOG000112548.
HOVERGENiHBG081522.
InParanoidiQ4G163.
KOiK10318.
OMAiFEVECIS.
OrthoDBiEOG783MXX.
PhylomeDBiQ4G163.
TreeFamiTF101170.

Family and domain databases

InterProiIPR002867. IBR_dom.
[Graphical view]
SMARTiSM00647. IBR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4G163-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFKDKDERI SCLEAYVTLT SKSSRFTDET EILKMSQRHS GQAGTEAGNG
60 70 80 90 100
ADSPPIVNSK YSTFRDFCST SSFQDSGYNE LKSCSFDNID KEYLGKKEKG
110 120 130 140 150
PTLLYEHPET SGLGLTHPLE SPTQKKKCIL PRKEKDKTPE LCETPKISGK
160 170 180 190 200
KCLPRRRLNV SFALLKGDFE SQNSSLESSI SQVINLEKNI PSSASGFSRA
210 220 230 240 250
NNFSPLVTST LKTEEVTSCS QKLRLNFSQQ KTSTIDDSKD DCSLFEVECI
260 270 280 290 300
SPIQGNNFKD SITHDFSDSS LCINDENACP ELLGSSVSGT TCGTDEDIFV
310 320 330 340 350
TPISNLVANI RFNASQILSP SPEVRGSIST PEDSGFNSLS LEKSEDSLSD
360 370 380 390 400
QEGSFQELLQ KHKGTPKVGD TIRKTRHLGR SRRLSTLREQ SSQSETEEEK
410 420 430 440 450
QIVHPDSEKR AAAASAISEG QLSSDESGDL TFSLKNLSKT PALQLVHELF
460 470 480 490 500
MKSKRKRLQE NSGHEFLEQG DGEKIAVLQC ILAGLIGKKM GIEKLDILTE
510 520 530 540 550
LKYRNLKHIL AMVLESLTAE SLCSVWKVSR NWREIVVQDK NANRRRKFYI
560 570 580 590 600
TQLKTDSEGA VLNVEDAATR LQLLNRSALR SVQAQARIPG SQREQGSTLS
610 620 630 640 650
PWGEVLTPLA SSSVTHLSSK QEEYVKVAKT LFTDEALKPC PRCQSPAKYQ
660 670 680 690 700
PYKKRGLCSR TACGFDFCVL CLCAYHGSEE CSRGAAKPRN RKDALPGSAQ

SKRNLKRL
Length:708
Mass (Da):78,402
Last modified:July 10, 2007 - v3
Checksum:iE42A358AE7BD29D1
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti139 – 1391P → L.
Corresponds to variant rs2279102 [ dbSNP | Ensembl ].
VAR_061168
Natural varianti139 – 1391P → R.
Corresponds to variant rs2279102 [ dbSNP | Ensembl ].
VAR_061169

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC021590 Genomic DNA. No translation available.
CCDSiCCDS47904.1.
RefSeqiNP_001025031.2. NM_001029860.3.
UniGeneiHs.339577.

Genome annotation databases

EnsembliENST00000428847; ENSP00000403293; ENSG00000156509.
GeneIDi286151.
KEGGihsa:286151.
UCSCiuc003yjd.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC021590 Genomic DNA. No translation available.
CCDSiCCDS47904.1.
RefSeqiNP_001025031.2. NM_001029860.3.
UniGeneiHs.339577.

3D structure databases

ProteinModelPortaliQ4G163.
SMRiQ4G163. Positions 633-684.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi130316. 2 interactions.
STRINGi9606.ENSP00000403293.

PTM databases

iPTMnetiQ4G163.

Polymorphism and mutation databases

BioMutaiFBXO43.
DMDMi152031603.

Proteomic databases

MaxQBiQ4G163.
PaxDbiQ4G163.
PRIDEiQ4G163.

Protocols and materials databases

DNASUi286151.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000428847; ENSP00000403293; ENSG00000156509.
GeneIDi286151.
KEGGihsa:286151.
UCSCiuc003yjd.4. human.

Organism-specific databases

CTDi286151.
GeneCardsiFBXO43.
H-InvDBHIX0034334.
HGNCiHGNC:28521. FBXO43.
HPAiHPA024292.
HPA024295.
HPA024824.
MIMi609110. gene.
neXtProtiNX_Q4G163.
PharmGKBiPA134913061.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IHWX. Eukaryota.
ENOG410XUB4. LUCA.
GeneTreeiENSGT00530000063692.
HOGENOMiHOG000112548.
HOVERGENiHBG081522.
InParanoidiQ4G163.
KOiK10318.
OMAiFEVECIS.
OrthoDBiEOG783MXX.
PhylomeDBiQ4G163.
TreeFamiTF101170.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

GenomeRNAii286151.
NextBioi96042.
PROiQ4G163.
SOURCEiSearch...

Gene expression databases

BgeeiQ4G163.
CleanExiHS_FBXO43.
ExpressionAtlasiQ4G163. baseline and differential.
GenevisibleiQ4G163. HS.

Family and domain databases

InterProiIPR002867. IBR_dom.
[Graphical view]
SMARTiSM00647. IBR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "DNA sequence and analysis of human chromosome 8."
    Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T.
    , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
    Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Systematic analysis and nomenclature of mammalian F-box proteins."
    Jin J., Cardozo T., Lovering R.C., Elledge S.J., Pagano M., Harper J.W.
    Genes Dev. 18:2573-2580(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE.
  3. "A role for the anaphase-promoting complex inhibitor Emi2/XErp1, a homolog of early mitotic inhibitor 1, in cytostatic factor arrest of Xenopus eggs."
    Tung J.J., Hansen D.V., Ban K.H., Loktev A.V., Summers M.K., Adler J.R. III, Jackson P.K.
    Proc. Natl. Acad. Sci. U.S.A. 102:4318-4323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION, PHOSPHORYLATION AT SER-76 AND SER-334, MUTAGENESIS OF 75-ASP-SER-76 AND 333-ASP-SER-334.
  4. "CaMKII and polo-like kinase 1 sequentially phosphorylate the cytostatic factor Emi2/XErp1 to trigger its destruction and meiotic exit."
    Hansen D.V., Tung J.J., Jackson P.K.
    Proc. Natl. Acad. Sci. U.S.A. 103:608-613(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT THR-234.

Entry informationi

Entry nameiFBX43_HUMAN
AccessioniPrimary (citable) accession number: Q4G163
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 10, 2007
Last modified: May 11, 2016
This is version 90 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.