Reviewed, UniProtKB/Swiss-Prot Q4G163 (FBX43_HUMAN)

Names and origin

Protein names

Recommended name:
    F-box only protein 43
Alternative name(s):
    Endogenous meiotic inhibitor 2

Gene names

Name:	FBXO43
Synonyms:	EMI2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	708 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required to establish and maintain the arrest of oocytes at the second meiotic metaphase until fertilization. Probably acts by inhibiting the anaphase-promoting complex/cyclosome (APC/C) ubiquitin ligase. Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation Probable.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Part of a SCF (SKP1-cullin-F-box) protein ligase complex. May interact with CDC20 By similarity.
Post-translational modification	Phosphorylated on Ser-76, Thr-234 and Ser-334 in reponse to calcium, which is a prerequisite for ubiquitination and proteasomal degradation Probable. Ubiquitinated in response to calcium, which promotes proteasomal degradation. Ref.3
Sequence similarities	Contains 1 F-box domain. Contains 1 IBR-type zinc finger.

Ontologies

Keywords
Biological process	Meiosis Ubl conjugation pathway
Domain	Zinc-finger
Ligand	Metal-binding Zinc
PTM	Phosphoprotein Ubl conjugation
Gene Ontology (GO)
Biological process	meiosis Inferred from electronic annotation. Source: UniProtKB-KW modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 708	708	F-box only protein 43		PRO_0000247233
Regions
	Domain	490 – 547	58	F-box
	Zinc finger	622 – 681	60	IBR-type
Amino acid modifications
	Modified residue	76	1	Phosphoserine Probable
	Modified residue	234	1	Phosphothreonine Ref.4
	Modified residue	334	1	Phosphoserine Probable
	Modified residue	503	1	Phosphotyrosine By similarity
Experimental info
	Mutagenesis	75 – 76	2	DS → AA: Impairs ubiquitination and degradation in response to calcium. Ref.3
	Mutagenesis	333 – 334	2	DS → AA: Impairs ubiquitination and degradation in response to calcium. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q4G163-1 [UniParc]. Last modified July 10, 2007. Version 3. Checksum: E42A358AE7BD29D1	FASTA	708	78,402
	10 20 30 40 50 60 MSFKDKDERI SCLEAYVTLT SKSSRFTDET EILKMSQRHS GQAGTEAGNG ADSPPIVNSK 70 80 90 100 110 120 YSTFRDFCST SSFQDSGYNE LKSCSFDNID KEYLGKKEKG PTLLYEHPET SGLGLTHPLE 130 140 150 160 170 180 SPTQKKKCIL PRKEKDKTPE LCETPKISGK KCLPRRRLNV SFALLKGDFE SQNSSLESSI 190 200 210 220 230 240 SQVINLEKNI PSSASGFSRA NNFSPLVTST LKTEEVTSCS QKLRLNFSQQ KTSTIDDSKD 250 260 270 280 290 300 DCSLFEVECI SPIQGNNFKD SITHDFSDSS LCINDENACP ELLGSSVSGT TCGTDEDIFV 310 320 330 340 350 360 TPISNLVANI RFNASQILSP SPEVRGSIST PEDSGFNSLS LEKSEDSLSD QEGSFQELLQ 370 380 390 400 410 420 KHKGTPKVGD TIRKTRHLGR SRRLSTLREQ SSQSETEEEK QIVHPDSEKR AAAASAISEG 430 440 450 460 470 480 QLSSDESGDL TFSLKNLSKT PALQLVHELF MKSKRKRLQE NSGHEFLEQG DGEKIAVLQC 490 500 510 520 530 540 ILAGLIGKKM GIEKLDILTE LKYRNLKHIL AMVLESLTAE SLCSVWKVSR NWREIVVQDK 550 560 570 580 590 600 NANRRRKFYI TQLKTDSEGA VLNVEDAATR LQLLNRSALR SVQAQARIPG SQREQGSTLS 610 620 630 640 650 660 PWGEVLTPLA SSSVTHLSSK QEEYVKVAKT LFTDEALKPC PRCQSPAKYQ PYKKRGLCSR 670 680 690 700 TACGFDFCVL CLCAYHGSEE CSRGAAKPRN RKDALPGSAQ SKRNLKRL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence and analysis of human chromosome 8." Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S. Nature 439:331-335(2006) [PubMed: 16421571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Systematic analysis and nomenclature of mammalian F-box proteins." Jin J., Cardozo T., Lovering R.C., Elledge S.J., Pagano M., Harper J.W. Genes Dev. 18:2573-2580(2004) [PubMed: 15520277] [Abstract] Cited for: NOMENCLATURE.
[3]	"A role for the anaphase-promoting complex inhibitor Emi2/XErp1, a homolog of early mitotic inhibitor 1, in cytostatic factor arrest of Xenopus eggs." Tung J.J., Hansen D.V., Ban K.H., Loktev A.V., Summers M.K., Adler J.R. III, Jackson P.K. Proc. Natl. Acad. Sci. U.S.A. 102:4318-4323(2005) [PubMed: 15753281] [Abstract] Cited for: UBIQUITINATION, PHOSPHORYLATION AT SER-76 AND SER-334, MUTAGENESIS OF 75-ASP-SER-76 AND 333-ASP-SER-334.
[4]	"CaMKII and polo-like kinase 1 sequentially phosphorylate the cytostatic factor Emi2/XErp1 to trigger its destruction and meiotic exit." Hansen D.V., Tung J.J., Jackson P.K. Proc. Natl. Acad. Sci. U.S.A. 103:608-613(2006) [PubMed: 16407128] [Abstract] Cited for: PHOSPHORYLATION AT THR-234.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC021590 Genomic DNA. No translation available.
IPI	IPI00175439.
RefSeq	NP_001025031.2.
UniGene	Hs.339577
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q4G163.
Proteomic databases
PRIDE	Q4G163.
Genome annotation databases
Ensembl	ENSG00000156509. Homo sapiens. [Contig view]
GeneID	286151.
KEGG	hsa:286151.
Organism-specific databases
GeneCards	GC08M101214.
HGNC	HGNC:28521. FBXO43.
MIM	609110. gene.
PharmGKB	PA134913061.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q4G163.
HOVERGEN	Q4G163.
Gene expression databases
ArrayExpress	Q4G163.
Bgee	Q4G163.
CleanEx	HS_FBXO43.
GermOnline	ENSG00000156509. Homo sapiens.
Family and domain databases
InterPro	IPR001810. F-box. IPR002867. Znf_C6HC. [Graphical view]
Pfam	PF00646. F-box. 1 hit. PF01485. IBR. 1 hit. [Graphical view]
SMART	SM00647. IBR. 1 hit. [Graphical view]
PROSITE	PS50181. FBOX. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	96042.
SOURCE	Search...

Entry information

Entry name

FBX43_HUMAN

Accession

Primary (citable) accession number: Q4G163

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	July 10, 2007
Last modified:	June 16, 2009
This is version 33 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)