ID   HSF5_HUMAN              Reviewed;         596 AA.
AC   Q4G112; Q08EH7; Q8N7V2;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   25-MAY-2022, sequence version 3.
DT   27-MAR-2024, entry version 152.
DE   RecName: Full=Heat shock factor protein 5;
DE            Short=HSF 5;
DE   AltName: Full=Heat shock transcription factor 5;
DE            Short=HSTF 5;
GN   Name=HSF5; Synonyms=HSTF5;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16625196; DOI=10.1038/nature04689;
RA   Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R.,
RA   Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A.,
RA   Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J.,
RA   Chang J.L., Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J.,
RA   DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S.,
RA   Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E.,
RA   Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K.,
RA   LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J.,
RA   Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A.,
RA   Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K.,
RA   Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D.,
RA   Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A.,
RA   Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.;
RT   "DNA sequence of human chromosome 17 and analysis of rearrangement in the
RT   human lineage.";
RL   Nature 440:1045-1049(2006).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 319-596 (ISOFORMS 1 AND 2).
RC   TISSUE=Testis;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
CC   -!- FUNCTION: May act as a transcriptional factor. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q4G112-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q4G112-2; Sequence=VSP_033454;
CC   -!- SIMILARITY: Belongs to the HSF family. {ECO:0000305}.
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DR   EMBL; AC005666; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AC023992; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC033017; AAH33017.1; -; mRNA.
DR   EMBL; BC033020; AAH33020.1; -; mRNA.
DR   EMBL; BC115385; AAI15386.1; -; mRNA.
DR   EMBL; AK097630; BAC05124.1; -; mRNA.
DR   CCDS; CCDS32690.1; -. [Q4G112-1]
DR   RefSeq; NP_001073908.2; NM_001080439.2. [Q4G112-1]
DR   AlphaFoldDB; Q4G112; -.
DR   SMR; Q4G112; -.
DR   BioGRID; 125871; 5.
DR   IntAct; Q4G112; 2.
DR   STRING; 9606.ENSP00000313243; -.
DR   iPTMnet; Q4G112; -.
DR   PhosphoSitePlus; Q4G112; -.
DR   BioMuta; HSF5; -.
DR   DMDM; 296434535; -.
DR   MassIVE; Q4G112; -.
DR   PaxDb; 9606-ENSP00000313243; -.
DR   PeptideAtlas; Q4G112; -.
DR   ProteomicsDB; 62150; -. [Q4G112-1]
DR   ProteomicsDB; 62151; -. [Q4G112-2]
DR   Antibodypedia; 18396; 113 antibodies from 23 providers.
DR   DNASU; 124535; -.
DR   Ensembl; ENST00000323777.8; ENSP00000313243.3; ENSG00000176160.11. [Q4G112-1]
DR   GeneID; 124535; -.
DR   KEGG; hsa:124535; -.
DR   MANE-Select; ENST00000323777.8; ENSP00000313243.3; NM_001080439.3; NP_001073908.2.
DR   UCSC; uc002iwi.2; human. [Q4G112-1]
DR   AGR; HGNC:26862; -.
DR   CTD; 124535; -.
DR   DisGeNET; 124535; -.
DR   GeneCards; HSF5; -.
DR   HGNC; HGNC:26862; HSF5.
DR   HPA; ENSG00000176160; Tissue enriched (testis).
DR   neXtProt; NX_Q4G112; -.
DR   OpenTargets; ENSG00000176160; -.
DR   PharmGKB; PA145008204; -.
DR   VEuPathDB; HostDB:ENSG00000176160; -.
DR   eggNOG; KOG1508; Eukaryota.
DR   GeneTree; ENSGT00510000048674; -.
DR   HOGENOM; CLU_037490_1_0_1; -.
DR   InParanoid; Q4G112; -.
DR   OMA; HRIWQNS; -.
DR   OrthoDB; 2908279at2759; -.
DR   PhylomeDB; Q4G112; -.
DR   TreeFam; TF330401; -.
DR   PathwayCommons; Q4G112; -.
DR   SignaLink; Q4G112; -.
DR   BioGRID-ORCS; 124535; 18 hits in 1153 CRISPR screens.
DR   ChiTaRS; HSF5; human.
DR   GenomeRNAi; 124535; -.
DR   Pharos; Q4G112; Tbio.
DR   PRO; PR:Q4G112; -.
DR   Proteomes; UP000005640; Chromosome 17.
DR   RNAct; Q4G112; Protein.
DR   Bgee; ENSG00000176160; Expressed in sperm and 34 other cell types or tissues.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:1990837; F:sequence-specific double-stranded DNA binding; IDA:ARUK-UCL.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR   InterPro; IPR000232; HSF_DNA-bd.
DR   InterPro; IPR027725; HSF_fam.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR10015:SF278; HEAT SHOCK FACTOR PROTEIN 5; 1.
DR   PANTHER; PTHR10015; HEAT SHOCK TRANSCRIPTION FACTOR; 1.
DR   Pfam; PF00447; HSF_DNA-bind; 1.
DR   SMART; SM00415; HSF; 1.
DR   SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
DR   Genevisible; Q4G112; HS.
PE   2: Evidence at transcript level;
KW   Alternative splicing; DNA-binding; Nucleus; Phosphoprotein;
KW   Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..596
FT                   /note="Heat shock factor protein 5"
FT                   /id="PRO_0000333041"
FT   DNA_BIND        10..200
FT                   /evidence="ECO:0000250"
FT   REGION          541..576
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         572
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q5ND04"
FT   VAR_SEQ         53..173
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_033454"
FT   VARIANT         329
FT                   /note="T -> N (in dbSNP:rs1017089)"
FT                   /id="VAR_043115"
FT   VARIANT         473
FT                   /note="S -> N (in dbSNP:rs3803752)"
FT                   /id="VAR_055935"
SQ   SEQUENCE   596 AA;  65265 MW;  8BD6D96DC0CF8962 CRC64;
     MEALLSTPIN PNNFPAKLWR LVNSPRYRSI RWDGRGEGLL IDQPLFEAEL LSPPGPGGGG
     GTAGAGAEPE LFKTTSFTSF IRQLNLYGFR KVVLGGPGGG KPAGNGPLHH FHNPHFRRDQ
     PQLLVHLKRL TSANKAKLAA GLEVPCRPPN RFQRLLITSA SAATAPLQHQ QPPPPAGPRP
     EPHGPVAVGQ FHRSFRRDSL SPYSCVSTPS HDHSTYPLKG LDRTPVPHRI WQNSLGMHPG
     QVETSPTFSD KGVPFPVLQR FPTEVTYTLQ PSTTSVHVQQ GPQTMVSSSQ KYSNYTPSAQ
     YSQAYYPTAV LQCCSPTHMD ALSSCVTPTA SSYAHCNYFQ NPSMQSSYPV EFLPSNWPCS
     TTDENTKTEV NLEAVFQIVD ELHSSPKLEM VKVEPVENQC PTSPSYRGQH ILANSNNSNP
     CSASQASQLE PLTPVGSDIM SFVVGTEQAV ACSLPQSPEY IYTIHTAQPV ENSTIQESAA
     IQQAHVKLKE HLNHNPSPSS VVFVQEGPPF STHQVDANIK CQTSSRENIL PSEQMGFLIS
     EMGPASKPSE DTGLATPARY REHRSNSQQG KSPDLHLLVD VACKQERFPK EEELKE
//