Q4G0N8 (SL9C1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sodium/hydrogen exchanger 10

Alternative name(s):
Na(+)/H(+) exchanger 10
Short name=NHE-10
Solute carrier family 9 member 10
Solute carrier family 9 member C1
Sperm-specific Na(+)/H(+) exchanger
Short name=sNHE

Gene names

Name:	SLC9C1
Synonyms:	SLC9A10

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1177 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Sperm-specific sodium/hydrogen exchanger involved in intracellular pH regulation of spermatozoa. Required for sperm motility and fertility. Involved in sperm cell hyperactivation, a step needed for sperm motility which is essential late in the preparation of sperm for fertilization. Required for the expression and bicarbonate regulation of the soluble adenylyl cyclase (sAC) By similarity.
Subunit structure	Interacts with soluble adenylyl cyclase (sAC) By similarity.
Subcellular location	Cell projection › cilium › flagellum membrane; Multi-pass membrane protein By similarity.
Domain	The ion transport-like region is related to the membrane segments of voltage-gated ion channels. Its function is unknown By similarity.
Sequence similarities	Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family. [View classification] Contains 1 cyclic nucleotide-binding domain.
Sequence caution	The sequence DAA01462.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Antiport Differentiation Ion transport Sodium transport Spermatogenesis Transport
Cellular component	Cell membrane Cell projection Cilium Flagellum Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane Transmembrane helix
Ligand	Sodium
Molecular function	Developmental protein
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW sodium ion transport Inferred from electronic annotation. Source: UniProtKB-KW sperm motility Inferred from electronic annotation. Source: Compara spermatogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cilium Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	solute:hydrogen antiporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q4G0N8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q4G0N8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     294-341: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1177	1177	Sodium/hydrogen exchanger 10		PRO_0000295704
Regions
	Transmembrane	8 – 28	21	Helical; Potential
	Transmembrane	39 – 59	21	Helical; Potential
	Transmembrane	73 – 93	21	Helical; Potential
	Transmembrane	103 – 123	21	Helical; Potential
	Transmembrane	138 – 158	21	Helical; Potential
	Transmembrane	167 – 187	21	Helical; Potential
	Transmembrane	204 – 224	21	Helical; Potential
	Transmembrane	248 – 268	21	Helical; Potential
	Transmembrane	301 – 321	21	Helical; Potential
	Transmembrane	333 – 353	21	Helical; Potential
	Transmembrane	364 – 384	21	Helical; Potential
	Transmembrane	405 – 425	21	Helical; Potential
	Transmembrane	613 – 633	21	Helical; Potential
	Transmembrane	646 – 666	21	Helical; Potential
	Transmembrane	675 – 695	21	Helical; Potential
	Transmembrane	707 – 727	21	Helical; Potential
	Nucleotide binding	870 – 1003	134	cNMP
	Region	598 – 678	81	Ion transport-like
Amino acid modifications
	Glycosylation	278	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	294 – 341	48	Missing in isoform 2.		VSP_027010
	Natural variant	158	1	I → V. Ref.1 Corresponds to variant rs9828502 [ dbSNP | Ensembl ].		VAR_033324
	Natural variant	286	1	I → V. Ref.1 Corresponds to variant rs9872691 [ dbSNP | Ensembl ].		VAR_033325
	Natural variant	348	1	I → M. Ref.1 Corresponds to variant rs9809404 [ dbSNP | Ensembl ].		VAR_033326
	Natural variant	364	1	I → V. Ref.1 Corresponds to variant rs9809384 [ dbSNP | Ensembl ].		VAR_050233
	Natural variant	424	1	T → A. Ref.1 Corresponds to variant rs6768523 [ dbSNP | Ensembl ].		VAR_033327
	Natural variant	705	1	T → I. Ref.1 Corresponds to variant rs4434123 [ dbSNP | Ensembl ].		VAR_033328
	Natural variant	732	1	Q → K. Ref.1 Corresponds to variant rs6781844 [ dbSNP | Ensembl ].		VAR_033329
	Natural variant	768	1	S → I. Ref.1 Corresponds to variant rs9288938 [ dbSNP | Ensembl ].		VAR_033330
	Natural variant	826	1	G → S. Ref.1 Corresponds to variant rs28516377 [ dbSNP | Ensembl ].		VAR_061369
Experimental info
	Sequence conflict	74	1	M → I in BAC87265. Ref.1
	Sequence conflict	785	1	E → D in BAC87265. Ref.1
	Sequence conflict	788	1	I → T in BAC87265. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 24, 2007. Version 2. Checksum: FD04B5B41D0EA2DC	FASTA	1,177	135,206
	10 20 30 40 50 60 MAGIFKEFFF STEDLPEVIL TLSLISSIGA FLNRHLEDFP IPVPVILFLL GCSFEVLSFT 70 80 90 100 110 120 SSQVQRYANA IQWMSPDLFF RIFTPVVFFT TAFDMDTYML QKLFWQILLI SIPGFLVNYI 130 140 150 160 170 180 LVLWHLASVN QLLLKPTQWL LFSAILVSSD PMLTAAAIRD LGLSRSLISL INGESLMTSV 190 200 210 220 230 240 ISLITFTSIM DFDQRLQSKR NHTLAEEIVG GICSYIIASF LFGILSSKLI QFWMSTVFGD 250 260 270 280 290 300 DVNHISLIFS ILYLIFYICE LVGMSGIFTL AIVGLLLNST SFKAAIEETL LLEFWTFLSR 310 320 330 340 350 360 IAFLMVFTFF GLLIPAHTYL YIEFVDIYYS LNIYLTLIVL RFLTLLLISP VLSRVGHEFS 370 380 390 400 410 420 WRWIFIMVCS EMKGMPNINM ALLLAYSDLY FGSDKEKSQI LFHGVLVCLI TLVVNRFILP 430 440 450 460 470 480 VAVTILGLRD ATSTKYKSVC CTFQHFQELT KSAASALKFD KDLANADWNM IEKAITLENP 490 500 510 520 530 540 YMLNEEETTE HQKVKCPHCN KEIDEIFNTE AMELANRRLL SAQIASYQRQ YRNEILSQSA 550 560 570 580 590 600 VQVLVGAAES FGEKKGKCMS LDTIKNYSES QKTVTFARKL LLNWVYNTRK EKEGPSKYFF 610 620 630 640 650 660 FRICHTIVFT EEFEHVGYLV ILMNIFPFII SWISQLNVIY HSELKHTNYC FLTLYILEAL 670 680 690 700 710 720 LKIAAMRKDF FSHAWNIFEL AITLIGILHV ILIEIDTIKY IFNETEVIVF IKVVQFFRIL 730 740 750 760 770 780 RIFKLIAPKL LQIIDKRMSH QKTFWYGILK GYVQGEADIM TIIDQITSSK QIKQMLLKQV 790 800 810 820 830 840 IRNMEHAIKE LGYLEYDHPE IAVTVKTKEE INVMLNMATE ILKAFGLKGI ISKTEGAGIN 850 860 870 880 890 900 KLIMAKKKEV LDSQSIIRPL TVEEVLYHIP WLDKNKDYIN FIQEKAKVVT FDCGNDIFEE 910 920 930 940 950 960 GDEPKGIYII ISGMVKLEKS KPGLGIDQMV ESKEKDFPII DTDYMLSGEI IGEINCLTNE 970 980 990 1000 1010 1020 PMKYSATCKT VVETCFIPKT HLYDAFEQCS PLIKQKMWLK LGLAITARKI REHLSYEDWN 1030 1040 1050 1060 1070 1080 YNMQLKLSNI YVVDIPMSTK TDIYDENLIY VILIHGAVED CLLRKTYRAP FLIPITCHQI 1090 1100 1110 1120 1130 1140 QSIEDFTKVV IIQTPINMKT FRRNIRKFVP KHKSYLTPGL IGSVGTLEEG IQEERNVKED 1150 1160 1170 GAHSAATARS PQPCSLLGTK FNCKESPRIN LRKVRKE
	Isoform 2 [UniParc]. Checksum: 1C290AB522BBA8D6	FASTA	1,129	129,365
	10 20 30 40 50 60 MAGIFKEFFF STEDLPEVIL TLSLISSIGA FLNRHLEDFP IPVPVILFLL GCSFEVLSFT 70 80 90 100 110 120 SSQVQRYANA IQWMSPDLFF RIFTPVVFFT TAFDMDTYML QKLFWQILLI SIPGFLVNYI 130 140 150 160 170 180 LVLWHLASVN QLLLKPTQWL LFSAILVSSD PMLTAAAIRD LGLSRSLISL INGESLMTSV 190 200 210 220 230 240 ISLITFTSIM DFDQRLQSKR NHTLAEEIVG GICSYIIASF LFGILSSKLI QFWMSTVFGD 250 260 270 280 290 300 DVNHISLIFS ILYLIFYICE LVGMSGIFTL AIVGLLLNST SFKAAIEETL LLEFLTLLLI 310 320 330 340 350 360 SPVLSRVGHE FSWRWIFIMV CSEMKGMPNI NMALLLAYSD LYFGSDKEKS QILFHGVLVC 370 380 390 400 410 420 LITLVVNRFI LPVAVTILGL RDATSTKYKS VCCTFQHFQE LTKSAASALK FDKDLANADW 430 440 450 460 470 480 NMIEKAITLE NPYMLNEEET TEHQKVKCPH CNKEIDEIFN TEAMELANRR LLSAQIASYQ 490 500 510 520 530 540 RQYRNEILSQ SAVQVLVGAA ESFGEKKGKC MSLDTIKNYS ESQKTVTFAR KLLLNWVYNT 550 560 570 580 590 600 RKEKEGPSKY FFFRICHTIV FTEEFEHVGY LVILMNIFPF IISWISQLNV IYHSELKHTN 610 620 630 640 650 660 YCFLTLYILE ALLKIAAMRK DFFSHAWNIF ELAITLIGIL HVILIEIDTI KYIFNETEVI 670 680 690 700 710 720 VFIKVVQFFR ILRIFKLIAP KLLQIIDKRM SHQKTFWYGI LKGYVQGEAD IMTIIDQITS 730 740 750 760 770 780 SKQIKQMLLK QVIRNMEHAI KELGYLEYDH PEIAVTVKTK EEINVMLNMA TEILKAFGLK 790 800 810 820 830 840 GIISKTEGAG INKLIMAKKK EVLDSQSIIR PLTVEEVLYH IPWLDKNKDY INFIQEKAKV 850 860 870 880 890 900 VTFDCGNDIF EEGDEPKGIY IIISGMVKLE KSKPGLGIDQ MVESKEKDFP IIDTDYMLSG 910 920 930 940 950 960 EIIGEINCLT NEPMKYSATC KTVVETCFIP KTHLYDAFEQ CSPLIKQKMW LKLGLAITAR 970 980 990 1000 1010 1020 KIREHLSYED WNYNMQLKLS NIYVVDIPMS TKTDIYDENL IYVILIHGAV EDCLLRKTYR 1030 1040 1050 1060 1070 1080 APFLIPITCH QIQSIEDFTK VVIIQTPINM KTFRRNIRKF VPKHKSYLTP GLIGSVGTLE 1090 1100 1110 1120 EGIQEERNVK EDGAHSAATA RSPQPCSLLG TKFNCKESPR INLRKVRKE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS VAL-158; VAL-286; MET-348; VAL-364; ALA-424; ILE-705; LYS-732; ILE-768 AND SER-826. Tissue: Testis.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Testis.
[3]	"Gene discovery in the hamster: a comparative genomics approach for gene annotation by sequencing of hamster testis cDNAs." Oduru S., Campbell J.L., Karri S., Hendry W.J., Khan S.A., Williams S.C. BMC Genomics 4:22-22(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK128084 mRNA. Translation: BAC87265.1. BC044801 mRNA. Translation: AAH44801.1. BK001328 mRNA. Translation: DAA01462.1. Sequence problems.
IPI	IPI00418791. IPI00645688.
RefSeq	NP_898884.1. NM_183061.1.
UniGene	Hs.680112.
3D structure databases
ProteinModelPortal	Q4G0N8.
SMR	Q4G0N8. Positions 888-1027.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000306627.
PTM databases
PhosphoSite	Q4G0N8.
Polymorphism databases
DMDM	158563886.
Proteomic databases
PaxDb	Q4G0N8.
PRIDE	Q4G0N8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000305815; ENSP00000306627; ENSG00000172139. ENST00000487372; ENSP00000420688; ENSG00000172139.
GeneID	285335.
KEGG	hsa:285335.
UCSC	uc003dyu.3. human. uc010hqc.3. human.
Organism-specific databases
CTD	285335.
GeneCards	GC03M111860.
H-InvDB	HIX0200547.
HGNC	HGNC:31401. SLC9C1.
HPA	HPA044853.
MIM	612738. gene.
neXtProt	NX_Q4G0N8.
PharmGKB	PA134914619.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG126655.
HOGENOM	HOG000169764.
HOVERGEN	HBG071202.
InParanoid	Q4G0N8.
KO	K14726.
OMA	PNINMAL.
OrthoDB	EOG4QNMVC.
Gene expression databases
ArrayExpress	Q4G0N8.
Bgee	Q4G0N8.
CleanEx	HS_SLC9A10.
Genevestigator	Q4G0N8.
Family and domain databases
Gene3D	2.60.120.10. 1 hit.
InterPro	IPR006153. Cation/H_exchanger. IPR018422. Cation/H_exchanger_CPA1. IPR018490. cNMP-bd-like. IPR000595. cNMP-bd_dom. IPR014710. RmlC-like_jellyroll. [Graphical view]
PANTHER	PTHR10110. PTHR10110. 1 hit.
Pfam	PF00027. cNMP_binding. 1 hit. PF00999. Na_H_Exchanger. 1 hit. [Graphical view]
SMART	SM00100. cNMP. 1 hit. [Graphical view]
SUPFAM	SSF51206. cNMP_binding. 1 hit.
PROSITE	PS00888. CNMP_BINDING_1. False negative. PS00889. CNMP_BINDING_2. False negative. PS50042. CNMP_BINDING_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SLC9C1. human.
GenomeRNAi	285335.
NextBio	95448.
SOURCE	Search...

Entry information

Entry name

SL9C1_HUMAN

Accession

Primary (citable) accession number: Q4G0N8
Secondary accession number(s): Q6ZRP4, Q7RTP2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	July 24, 2007
Last modified:	May 1, 2013
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.