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Q4G0N4 (NAKD2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NAD kinase 2, mitochondrial

EC=2.7.1.23
Alternative name(s):
Mitochondrial NAD kinase
NAD kinase domain-containing protein 1, mitochondrial
Gene names
Name:NADK2
Synonyms:C5orf33, MNADK, NADKD1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length442 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mitochondrial NAD+ kinase that phosphorylates NAD+ to yield NADP+. Can use both ATP or inorganic polyphosphate as the phosphoryl donor. Also has weak NADH kinase activity in vitro; however NADH kinase activity is much weaker than the NAD+ kinase activity and may not be relevant in vivo. Ref.6

Catalytic activity

ATP + NAD+ = ADP + NADP+. Ref.6

Enzyme regulation

Inhibited by NADH, NADPH and NADP+. Ref.6

Subunit structure

Homodimer Probable. Ref.6

Subcellular location

Mitochondrion Ref.6.

Tissue specificity

Widely expressed. Ref.6

Sequence similarities

Belongs to the NAD kinase family.

Biophysicochemical properties

Kinetic parameters:

KM=0.022 mM for NAD+ Ref.6

KM=1.7 mM for ATP

KM=1.2 mM for tetrapolyphosphate

Vmax=0.091 µmol/min/mg enzyme

Ontologies

Keywords
   Cellular componentMitochondrion
   Coding sequence diversityAlternative splicing
   DomainTransit peptide
   LigandATP-binding
NAD
NADP
Nucleotide-binding
   Molecular functionKinase
Transferase
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processNAD metabolic process

Inferred from direct assay Ref.6. Source: UniProtKB

NADP biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentmitochondrion

Inferred from direct assay Ref.6. Source: UniProtKB

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

NAD+ kinase activity

Inferred from direct assay Ref.6. Source: UniProtKB

protein homodimerization activity

Inferred from direct assay Ref.6. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q4G0N4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q4G0N4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     288-319: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q4G0N4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-163: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 6262Mitochondrion Potential
Chain63 – 442380NAD kinase 2, mitochondrial
PRO_0000296292

Amino acid modifications

Modified residue761N6-acetyllysine; alternate By similarity
Modified residue761N6-succinyllysine; alternate By similarity
Modified residue3021N6-succinyllysine By similarity
Modified residue3171N6-acetyllysine; alternate By similarity
Modified residue3171N6-succinyllysine; alternate By similarity
Modified residue3971N6-acetyllysine By similarity

Natural variations

Alternative sequence1 – 163163Missing in isoform 3.
VSP_027192
Alternative sequence288 – 31932Missing in isoform 2.
VSP_027193

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 24, 2007. Version 2.
Checksum: 289EA16979AF14D8

FASTA44249,433
        10         20         30         40         50         60 
MTCYRGFLLG SCCRVAGGRA AALRGPGAGG PAARPRLGGD GGGRRHLGQG QPRELAGCGS 

        70         80         90        100        110        120 
RADGGFRPSR VVVVAKTTRY EFEQQRYRYA ELSEEDLKQL LALKGSSYSG LLERHHIHTK 

       130        140        150        160        170        180 
NVEHIIDSLR NEGIEVRLVK RREYDEETVR WADAVIAAGG DGTMLLAASK VLDRLKPVIG 

       190        200        210        220        230        240 
VNTDPERSEG HLCLPVRYTH SFPEALQKFY RGEFRWLWRQ RIRLYLEGTG INPVPVDLHE 

       250        260        270        280        290        300 
QQLSLNQHNR ALNIERAHDE RSEASGPQLL PVRALNEVFI GESLSSRASY YEISVDDGPW 

       310        320        330        340        350        360 
EKQKSSGLNL CTGTGSKAWS FNINRVATQA VEDVLNIAKR QGNLSLPLNR ELVEKVTNEY 

       370        380        390        400        410        420 
NESLLYSPEE PKILFSIREP IANRVFSSSR QRCFSSKVCV RSRCWDACMV VDGGTSFEFN 

       430        440 
DGAIASMMIN KEDELRTVLL EQ 

« Hide

Isoform 2 [UniParc].

Checksum: BAED88797577AAC2
Show »

FASTA41045,972
Isoform 3 [UniParc].

Checksum: 8A6977E19682F747
Show »

FASTA27931,716

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Brain.
[2]"The DNA sequence and comparative analysis of human chromosome 5."
Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. expand/collapse author list , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 39-442 (ISOFORM 1).
Tissue: Brain.
[4]Li H., Yu R., Zhou G., Shen C., Xiao W., Li M., Ke R., Zhong G., Lin L., Yang S.
Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 164-442 (ISOFORM 2).
[5]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[6]"Identification and characterization of a human mitochondrial NAD kinase."
Ohashi K., Kawai S., Murata K.
Nat. Commun. 3:1248-1248(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, ENZYME REGULATION, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK055158 mRNA. Translation: BAB70864.1.
AC008807 Genomic DNA. No translation available.
AC008942 Genomic DNA. No translation available.
BC045565 mRNA. No translation available.
BC062567 mRNA. Translation: AAH62567.1.
AY360463 mRNA. Translation: AAQ62967.1.
CCDSCCDS3917.1. [Q4G0N4-3]
CCDS47197.1. [Q4G0N4-1]
RefSeqNP_001078880.1. NM_001085411.2. [Q4G0N4-1]
NP_001274269.1. NM_001287340.1. [Q4G0N4-3]
NP_001274270.1. NM_001287341.1.
NP_694558.1. NM_153013.4. [Q4G0N4-3]
UniGeneHs.81907.

3D structure databases

ProteinModelPortalQ4G0N4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid126369. 17 interactions.
MINTMINT-3056473.

PTM databases

PhosphoSiteQ4G0N4.

Polymorphism databases

DMDM156630863.

Proteomic databases

MaxQBQ4G0N4.
PaxDbQ4G0N4.
PRIDEQ4G0N4.

Protocols and materials databases

DNASU133686.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000282512; ENSP00000282512; ENSG00000152620. [Q4G0N4-3]
ENST00000381937; ENSP00000371362; ENSG00000152620. [Q4G0N4-1]
ENST00000397338; ENSP00000380499; ENSG00000152620. [Q4G0N4-3]
ENST00000514504; ENSP00000421029; ENSG00000152620. [Q4G0N4-2]
GeneID133686.
KEGGhsa:133686.
UCSCuc003jkf.4. human. [Q4G0N4-1]
uc010iux.3. human. [Q4G0N4-2]

Organism-specific databases

CTD133686.
GeneCardsGC05M036194.
H-InvDBHIX0004812.
HGNCHGNC:26404. NADK2.
HPAHPA038367.
neXtProtNX_Q4G0N4.
PharmGKBPA162380039.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG240615.
HOVERGENHBG106388.
InParanoidQ4G0N4.
OMAVRWADAI.
OrthoDBEOG7FJH14.
PhylomeDBQ4G0N4.
TreeFamTF314077.

Gene expression databases

ArrayExpressQ4G0N4.
BgeeQ4G0N4.
CleanExHS_C5orf33.
GenevestigatorQ4G0N4.

Family and domain databases

Gene3D2.60.200.30. 2 hits.
3.40.50.10330. 1 hit.
InterProIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR012355. NADK_euk.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PfamPF01513. NAD_kinase. 1 hit.
[Graphical view]
PIRSFPIRSF017565. Kin_ATP-NAD_euk. 1 hit.
SUPFAMSSF111331. SSF111331. 2 hits.
ProtoNetSearch...

Other

ChiTaRSNADKD1. human.
GenomeRNAi133686.
NextBio83260.
PROQ4G0N4.

Entry information

Entry nameNAKD2_HUMAN
AccessionPrimary (citable) accession number: Q4G0N4
Secondary accession number(s): B5MC93, Q6UTX5, Q96NM0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: July 24, 2007
Last modified: July 9, 2014
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Uncharacterized protein families (UPF)

List of uncharacterized protein family (UPF) entries

SIMILARITY comments

Index of protein domains and families

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM