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Q4G0N4

- NAKD2_HUMAN

UniProt

Q4G0N4 - NAKD2_HUMAN

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Protein

NAD kinase 2, mitochondrial

Gene
NADK2, C5orf33, MNADK, NADKD1
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Mitochondrial NAD+ kinase that phosphorylates NAD+ to yield NADP+. Can use both ATP or inorganic polyphosphate as the phosphoryl donor. Also has weak NADH kinase activity in vitro; however NADH kinase activity is much weaker than the NAD+ kinase activity and may not be relevant in vivo.1 Publication

Catalytic activityi

ATP + NAD+ = ADP + NADP+.1 Publication

Enzyme regulationi

Inhibited by NADH, NADPH and NADP+.1 Publication

Kineticsi

  1. KM=0.022 mM for NAD+1 Publication
  2. KM=1.7 mM for ATP
  3. KM=1.2 mM for tetrapolyphosphate

Vmax=0.091 µmol/min/mg enzyme

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. NAD+ kinase activity Source: UniProtKB
  3. protein homodimerization activity Source: UniProtKB

GO - Biological processi

  1. NAD metabolic process Source: UniProtKB
  2. NADP biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinase 2, mitochondrial (EC:2.7.1.23)
Alternative name(s):
Mitochondrial NAD kinase
NAD kinase domain-containing protein 1, mitochondrial
Gene namesi
Name:NADK2
Synonyms:C5orf33, MNADK, NADKD1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 5

Organism-specific databases

HGNCiHGNC:26404. NADK2.

Subcellular locationi

Mitochondrion 1 Publication

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162380039.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 6262Mitochondrion Reviewed predictionAdd
BLAST
Chaini63 – 442380NAD kinase 2, mitochondrialPRO_0000296292Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei76 – 761N6-acetyllysine; alternate By similarity
Modified residuei76 – 761N6-succinyllysine; alternate By similarity
Modified residuei302 – 3021N6-succinyllysine By similarity
Modified residuei317 – 3171N6-acetyllysine; alternate By similarity
Modified residuei317 – 3171N6-succinyllysine; alternate By similarity
Modified residuei397 – 3971N6-acetyllysine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ4G0N4.
PaxDbiQ4G0N4.
PRIDEiQ4G0N4.

PTM databases

PhosphoSiteiQ4G0N4.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

ArrayExpressiQ4G0N4.
BgeeiQ4G0N4.
CleanExiHS_C5orf33.
GenevestigatoriQ4G0N4.

Organism-specific databases

HPAiHPA038367.

Interactioni

Subunit structurei

Homodimer Inferred.1 Publication

Protein-protein interaction databases

BioGridi126369. 17 interactions.
MINTiMINT-3056473.

Structurei

3D structure databases

ProteinModelPortaliQ4G0N4.

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiNOG240615.
HOVERGENiHBG106388.
InParanoidiQ4G0N4.
OMAiVRWADAI.
OrthoDBiEOG7FJH14.
PhylomeDBiQ4G0N4.
TreeFamiTF314077.

Family and domain databases

Gene3Di2.60.200.30. 2 hits.
3.40.50.10330. 1 hit.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR012355. NADK_euk.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF017565. Kin_ATP-NAD_euk. 1 hit.
SUPFAMiSSF111331. SSF111331. 2 hits.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q4G0N4-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MTCYRGFLLG SCCRVAGGRA AALRGPGAGG PAARPRLGGD GGGRRHLGQG    50
QPRELAGCGS RADGGFRPSR VVVVAKTTRY EFEQQRYRYA ELSEEDLKQL 100
LALKGSSYSG LLERHHIHTK NVEHIIDSLR NEGIEVRLVK RREYDEETVR 150
WADAVIAAGG DGTMLLAASK VLDRLKPVIG VNTDPERSEG HLCLPVRYTH 200
SFPEALQKFY RGEFRWLWRQ RIRLYLEGTG INPVPVDLHE QQLSLNQHNR 250
ALNIERAHDE RSEASGPQLL PVRALNEVFI GESLSSRASY YEISVDDGPW 300
EKQKSSGLNL CTGTGSKAWS FNINRVATQA VEDVLNIAKR QGNLSLPLNR 350
ELVEKVTNEY NESLLYSPEE PKILFSIREP IANRVFSSSR QRCFSSKVCV 400
RSRCWDACMV VDGGTSFEFN DGAIASMMIN KEDELRTVLL EQ 442
Length:442
Mass (Da):49,433
Last modified:July 24, 2007 - v2
Checksum:i289EA16979AF14D8
GO
Isoform 2 (identifier: Q4G0N4-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     288-319: Missing.

Note: No experimental confirmation available.

Show »
Length:410
Mass (Da):45,972
Checksum:iBAED88797577AAC2
GO
Isoform 3 (identifier: Q4G0N4-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-163: Missing.

Show »
Length:279
Mass (Da):31,716
Checksum:i8A6977E19682F747
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 163163Missing in isoform 3. VSP_027192Add
BLAST
Alternative sequencei288 – 31932Missing in isoform 2. VSP_027193Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK055158 mRNA. Translation: BAB70864.1.
AC008807 Genomic DNA. No translation available.
AC008942 Genomic DNA. No translation available.
BC045565 mRNA. No translation available.
BC062567 mRNA. Translation: AAH62567.1.
AY360463 mRNA. Translation: AAQ62967.1.
CCDSiCCDS3917.1. [Q4G0N4-3]
CCDS47197.1. [Q4G0N4-1]
RefSeqiNP_001078880.1. NM_001085411.2. [Q4G0N4-1]
NP_001274269.1. NM_001287340.1. [Q4G0N4-3]
NP_001274270.1. NM_001287341.1.
NP_694558.1. NM_153013.4. [Q4G0N4-3]
UniGeneiHs.81907.

Genome annotation databases

EnsembliENST00000282512; ENSP00000282512; ENSG00000152620. [Q4G0N4-3]
ENST00000381937; ENSP00000371362; ENSG00000152620. [Q4G0N4-1]
ENST00000397338; ENSP00000380499; ENSG00000152620. [Q4G0N4-3]
ENST00000514504; ENSP00000421029; ENSG00000152620. [Q4G0N4-2]
GeneIDi133686.
KEGGihsa:133686.
UCSCiuc003jkf.4. human. [Q4G0N4-1]
uc010iux.3. human. [Q4G0N4-2]

Polymorphism databases

DMDMi156630863.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK055158 mRNA. Translation: BAB70864.1 .
AC008807 Genomic DNA. No translation available.
AC008942 Genomic DNA. No translation available.
BC045565 mRNA. No translation available.
BC062567 mRNA. Translation: AAH62567.1 .
AY360463 mRNA. Translation: AAQ62967.1 .
CCDSi CCDS3917.1. [Q4G0N4-3 ]
CCDS47197.1. [Q4G0N4-1 ]
RefSeqi NP_001078880.1. NM_001085411.2. [Q4G0N4-1 ]
NP_001274269.1. NM_001287340.1. [Q4G0N4-3 ]
NP_001274270.1. NM_001287341.1.
NP_694558.1. NM_153013.4. [Q4G0N4-3 ]
UniGenei Hs.81907.

3D structure databases

ProteinModelPortali Q4G0N4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 126369. 17 interactions.
MINTi MINT-3056473.

PTM databases

PhosphoSitei Q4G0N4.

Polymorphism databases

DMDMi 156630863.

Proteomic databases

MaxQBi Q4G0N4.
PaxDbi Q4G0N4.
PRIDEi Q4G0N4.

Protocols and materials databases

DNASUi 133686.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000282512 ; ENSP00000282512 ; ENSG00000152620 . [Q4G0N4-3 ]
ENST00000381937 ; ENSP00000371362 ; ENSG00000152620 . [Q4G0N4-1 ]
ENST00000397338 ; ENSP00000380499 ; ENSG00000152620 . [Q4G0N4-3 ]
ENST00000514504 ; ENSP00000421029 ; ENSG00000152620 . [Q4G0N4-2 ]
GeneIDi 133686.
KEGGi hsa:133686.
UCSCi uc003jkf.4. human. [Q4G0N4-1 ]
uc010iux.3. human. [Q4G0N4-2 ]

Organism-specific databases

CTDi 133686.
GeneCardsi GC05M036194.
H-InvDB HIX0004812.
HGNCi HGNC:26404. NADK2.
HPAi HPA038367.
MIMi 615787. gene.
neXtProti NX_Q4G0N4.
PharmGKBi PA162380039.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG240615.
HOVERGENi HBG106388.
InParanoidi Q4G0N4.
OMAi VRWADAI.
OrthoDBi EOG7FJH14.
PhylomeDBi Q4G0N4.
TreeFami TF314077.

Miscellaneous databases

ChiTaRSi NADKD1. human.
GenomeRNAii 133686.
NextBioi 83260.
PROi Q4G0N4.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q4G0N4.
Bgeei Q4G0N4.
CleanExi HS_C5orf33.
Genevestigatori Q4G0N4.

Family and domain databases

Gene3Di 2.60.200.30. 2 hits.
3.40.50.10330. 1 hit.
InterProi IPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR012355. NADK_euk.
IPR002504. PolyP/ATP_NADK.
[Graphical view ]
Pfami PF01513. NAD_kinase. 1 hit.
[Graphical view ]
PIRSFi PIRSF017565. Kin_ATP-NAD_euk. 1 hit.
SUPFAMi SSF111331. SSF111331. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Brain.
  2. "The DNA sequence and comparative analysis of human chromosome 5."
    Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.
    , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
    Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 39-442 (ISOFORM 1).
    Tissue: Brain.
  4. Li H., Yu R., Zhou G., Shen C., Xiao W., Li M., Ke R., Zhong G., Lin L., Yang S.
    Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 164-442 (ISOFORM 2).
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "Identification and characterization of a human mitochondrial NAD kinase."
    Ohashi K., Kawai S., Murata K.
    Nat. Commun. 3:1248-1248(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, ENZYME REGULATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiNAKD2_HUMAN
AccessioniPrimary (citable) accession number: Q4G0N4
Secondary accession number(s): B5MC93, Q6UTX5, Q96NM0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: July 24, 2007
Last modified: September 3, 2014
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families
  4. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

External Data

Dasty 3

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