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Q4G0N4

- NAKD2_HUMAN

UniProt

Q4G0N4 - NAKD2_HUMAN

Protein

NAD kinase 2, mitochondrial

Gene

NADK2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 2 (24 Jul 2007)
      Previous versions | rss
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    Functioni

    Mitochondrial NAD+ kinase that phosphorylates NAD+ to yield NADP+. Can use both ATP or inorganic polyphosphate as the phosphoryl donor. Also has weak NADH kinase activity in vitro; however NADH kinase activity is much weaker than the NAD+ kinase activity and may not be relevant in vivo.1 Publication

    Catalytic activityi

    ATP + NAD+ = ADP + NADP+.1 Publication

    Enzyme regulationi

    Inhibited by NADH, NADPH and NADP+.1 Publication

    Kineticsi

    1. KM=0.022 mM for NAD+1 Publication
    2. KM=1.7 mM for ATP1 Publication
    3. KM=1.2 mM for tetrapolyphosphate1 Publication

    Vmax=0.091 µmol/min/mg enzyme1 Publication

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. NAD+ kinase activity Source: UniProtKB
    3. protein homodimerization activity Source: UniProtKB

    GO - Biological processi

    1. NAD metabolic process Source: UniProtKB
    2. NADP biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, NAD, NADP, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NAD kinase 2, mitochondrial (EC:2.7.1.23)
    Alternative name(s):
    Mitochondrial NAD kinase
    NAD kinase domain-containing protein 1, mitochondrial
    Gene namesi
    Name:NADK2
    Synonyms:C5orf33, MNADK, NADKD1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 5

    Organism-specific databases

    HGNCiHGNC:26404. NADK2.

    Subcellular locationi

    Mitochondrion 1 Publication

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB

    Keywords - Cellular componenti

    Mitochondrion

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA162380039.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 6262MitochondrionSequence AnalysisAdd
    BLAST
    Chaini63 – 442380NAD kinase 2, mitochondrialPRO_0000296292Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei76 – 761N6-acetyllysine; alternateBy similarity
    Modified residuei76 – 761N6-succinyllysine; alternateBy similarity
    Modified residuei302 – 3021N6-succinyllysineBy similarity
    Modified residuei317 – 3171N6-acetyllysine; alternateBy similarity
    Modified residuei317 – 3171N6-succinyllysine; alternateBy similarity
    Modified residuei397 – 3971N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ4G0N4.
    PaxDbiQ4G0N4.
    PRIDEiQ4G0N4.

    PTM databases

    PhosphoSiteiQ4G0N4.

    Expressioni

    Tissue specificityi

    Widely expressed.1 Publication

    Gene expression databases

    ArrayExpressiQ4G0N4.
    BgeeiQ4G0N4.
    CleanExiHS_C5orf33.
    GenevestigatoriQ4G0N4.

    Organism-specific databases

    HPAiHPA038367.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    BioGridi126369. 17 interactions.
    MINTiMINT-3056473.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4G0N4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NAD kinase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiNOG240615.
    HOVERGENiHBG106388.
    InParanoidiQ4G0N4.
    OMAiVRWADAI.
    OrthoDBiEOG7FJH14.
    PhylomeDBiQ4G0N4.
    TreeFamiTF314077.

    Family and domain databases

    Gene3Di2.60.200.30. 2 hits.
    3.40.50.10330. 1 hit.
    InterProiIPR017438. ATP-NAD_kinase_dom_1.
    IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
    IPR012355. NADK_euk.
    IPR002504. PolyP/ATP_NADK.
    [Graphical view]
    PfamiPF01513. NAD_kinase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF017565. Kin_ATP-NAD_euk. 1 hit.
    SUPFAMiSSF111331. SSF111331. 2 hits.

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q4G0N4-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTCYRGFLLG SCCRVAGGRA AALRGPGAGG PAARPRLGGD GGGRRHLGQG    50
    QPRELAGCGS RADGGFRPSR VVVVAKTTRY EFEQQRYRYA ELSEEDLKQL 100
    LALKGSSYSG LLERHHIHTK NVEHIIDSLR NEGIEVRLVK RREYDEETVR 150
    WADAVIAAGG DGTMLLAASK VLDRLKPVIG VNTDPERSEG HLCLPVRYTH 200
    SFPEALQKFY RGEFRWLWRQ RIRLYLEGTG INPVPVDLHE QQLSLNQHNR 250
    ALNIERAHDE RSEASGPQLL PVRALNEVFI GESLSSRASY YEISVDDGPW 300
    EKQKSSGLNL CTGTGSKAWS FNINRVATQA VEDVLNIAKR QGNLSLPLNR 350
    ELVEKVTNEY NESLLYSPEE PKILFSIREP IANRVFSSSR QRCFSSKVCV 400
    RSRCWDACMV VDGGTSFEFN DGAIASMMIN KEDELRTVLL EQ 442
    Length:442
    Mass (Da):49,433
    Last modified:July 24, 2007 - v2
    Checksum:i289EA16979AF14D8
    GO
    Isoform 2 (identifier: Q4G0N4-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         288-319: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:410
    Mass (Da):45,972
    Checksum:iBAED88797577AAC2
    GO
    Isoform 3 (identifier: Q4G0N4-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-163: Missing.

    Show »
    Length:279
    Mass (Da):31,716
    Checksum:i8A6977E19682F747
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 163163Missing in isoform 3. 2 PublicationsVSP_027192Add
    BLAST
    Alternative sequencei288 – 31932Missing in isoform 2. 1 PublicationVSP_027193Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK055158 mRNA. Translation: BAB70864.1.
    AC008807 Genomic DNA. No translation available.
    AC008942 Genomic DNA. No translation available.
    BC045565 mRNA. No translation available.
    BC062567 mRNA. Translation: AAH62567.1.
    AY360463 mRNA. Translation: AAQ62967.1.
    CCDSiCCDS3917.1. [Q4G0N4-3]
    CCDS47197.1. [Q4G0N4-1]
    RefSeqiNP_001078880.1. NM_001085411.2. [Q4G0N4-1]
    NP_001274269.1. NM_001287340.1. [Q4G0N4-3]
    NP_001274270.1. NM_001287341.1.
    NP_694558.1. NM_153013.4. [Q4G0N4-3]
    UniGeneiHs.81907.

    Genome annotation databases

    EnsembliENST00000282512; ENSP00000282512; ENSG00000152620. [Q4G0N4-3]
    ENST00000381937; ENSP00000371362; ENSG00000152620. [Q4G0N4-1]
    ENST00000397338; ENSP00000380499; ENSG00000152620. [Q4G0N4-3]
    ENST00000514504; ENSP00000421029; ENSG00000152620. [Q4G0N4-2]
    GeneIDi133686.
    KEGGihsa:133686.
    UCSCiuc003jkf.4. human. [Q4G0N4-1]
    uc010iux.3. human. [Q4G0N4-2]

    Polymorphism databases

    DMDMi156630863.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK055158 mRNA. Translation: BAB70864.1 .
    AC008807 Genomic DNA. No translation available.
    AC008942 Genomic DNA. No translation available.
    BC045565 mRNA. No translation available.
    BC062567 mRNA. Translation: AAH62567.1 .
    AY360463 mRNA. Translation: AAQ62967.1 .
    CCDSi CCDS3917.1. [Q4G0N4-3 ]
    CCDS47197.1. [Q4G0N4-1 ]
    RefSeqi NP_001078880.1. NM_001085411.2. [Q4G0N4-1 ]
    NP_001274269.1. NM_001287340.1. [Q4G0N4-3 ]
    NP_001274270.1. NM_001287341.1.
    NP_694558.1. NM_153013.4. [Q4G0N4-3 ]
    UniGenei Hs.81907.

    3D structure databases

    ProteinModelPortali Q4G0N4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 126369. 17 interactions.
    MINTi MINT-3056473.

    PTM databases

    PhosphoSitei Q4G0N4.

    Polymorphism databases

    DMDMi 156630863.

    Proteomic databases

    MaxQBi Q4G0N4.
    PaxDbi Q4G0N4.
    PRIDEi Q4G0N4.

    Protocols and materials databases

    DNASUi 133686.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000282512 ; ENSP00000282512 ; ENSG00000152620 . [Q4G0N4-3 ]
    ENST00000381937 ; ENSP00000371362 ; ENSG00000152620 . [Q4G0N4-1 ]
    ENST00000397338 ; ENSP00000380499 ; ENSG00000152620 . [Q4G0N4-3 ]
    ENST00000514504 ; ENSP00000421029 ; ENSG00000152620 . [Q4G0N4-2 ]
    GeneIDi 133686.
    KEGGi hsa:133686.
    UCSCi uc003jkf.4. human. [Q4G0N4-1 ]
    uc010iux.3. human. [Q4G0N4-2 ]

    Organism-specific databases

    CTDi 133686.
    GeneCardsi GC05M036194.
    H-InvDB HIX0004812.
    HGNCi HGNC:26404. NADK2.
    HPAi HPA038367.
    MIMi 615787. gene.
    neXtProti NX_Q4G0N4.
    PharmGKBi PA162380039.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG240615.
    HOVERGENi HBG106388.
    InParanoidi Q4G0N4.
    OMAi VRWADAI.
    OrthoDBi EOG7FJH14.
    PhylomeDBi Q4G0N4.
    TreeFami TF314077.

    Miscellaneous databases

    ChiTaRSi NADKD1. human.
    GenomeRNAii 133686.
    NextBioi 83260.
    PROi Q4G0N4.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q4G0N4.
    Bgeei Q4G0N4.
    CleanExi HS_C5orf33.
    Genevestigatori Q4G0N4.

    Family and domain databases

    Gene3Di 2.60.200.30. 2 hits.
    3.40.50.10330. 1 hit.
    InterProi IPR017438. ATP-NAD_kinase_dom_1.
    IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
    IPR012355. NADK_euk.
    IPR002504. PolyP/ATP_NADK.
    [Graphical view ]
    Pfami PF01513. NAD_kinase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF017565. Kin_ATP-NAD_euk. 1 hit.
    SUPFAMi SSF111331. SSF111331. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
      Tissue: Brain.
    2. "The DNA sequence and comparative analysis of human chromosome 5."
      Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.
      , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
      Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 39-442 (ISOFORM 1).
      Tissue: Brain.
    4. Li H., Yu R., Zhou G., Shen C., Xiao W., Li M., Ke R., Zhong G., Lin L., Yang S.
      Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 164-442 (ISOFORM 2).
    5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    6. "Identification and characterization of a human mitochondrial NAD kinase."
      Ohashi K., Kawai S., Murata K.
      Nat. Commun. 3:1248-1248(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, ENZYME REGULATION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiNAKD2_HUMAN
    AccessioniPrimary (citable) accession number: Q4G0N4
    Secondary accession number(s): B5MC93, Q6UTX5, Q96NM0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 24, 2007
    Last sequence update: July 24, 2007
    Last modified: October 1, 2014
    This is version 76 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families
    4. Uncharacterized protein families (UPF)
      List of uncharacterized protein family (UPF) entries

    External Data

    Dasty 3