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Reviewed, UniProtKB/Swiss-Prot Q4G0J3 (LARP7_HUMAN)

Last modified July 7, 2009. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    La-related protein 7
Alternative name(s):
    La ribonucleoprotein domain family member 7
    P-TEFb-interaction protein for 7SK stability
      Short name=PIP7S
Gene names
Name: LARP7
ORF Names: HDCMA18P
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length582 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Negative transcriptional regulator of polymerase II genes, acting by means of the 7SK RNP system. Within the 7SK RNP complex, the positive transcription elongation factor b (P-TEFb) is sequestered in an inactive form, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. Ref.1 Ref.6

Subunit structure

Integral part of the 7SK RNP complex. Specifically binds to the highly conserved 3'-terminal U-rich stretch of 7SK RNA. On stimulation, remains associated with 7SK RNA, whereas P-TEFb is released from the complex.

Subcellular location

Nucleusnucleoplasm. Ref.1

Post-translational modification

Phosphorylated upon DNA damage, probably by ATM or ATR By similarity.

Sequence similarities

Contains 1 HTH La-type RNA-binding domain.

Contains 1 RRM (RNA recognition motif) domain.

Sequence caution

The sequence ACD13786.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

The sequence EAX06284.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   LigandRNA-binding
   PTMPhosphoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processRNA processing

Inferred from electronic annotation. Source: InterPro

   Cellular componentnucleoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

ribonucleoprotein complex

Inferred from electronic annotation. Source: InterPro

   Molecular functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

nucleotide binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q4G0J3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q4G0J3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-368: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 582582La-related protein 7
PRO_0000281143

Regions

Domain28 – 12295HTH La-type RNA-binding
Domain125 – 20379RRM
Compositional bias202 – 368167Lys-rich

Amino acid modifications

Modified residue2571Phosphothreonine Ref.8
Modified residue2581Phosphoserine Ref.8
Modified residue2611Phosphoserine Ref.8
Modified residue2731Phosphoserine Ref.8
Modified residue3371Phosphoserine Ref.8 Ref.7 Ref.9
Modified residue3381Phosphothreonine Ref.8 Ref.7 Ref.9
Modified residue4401Phosphothreonine By similarity

Natural variations

Alternative sequence1 – 368368Missing in isoform 2.
VSP_024021

Experimental info

Mutagenesis1281Y → D: Loss of 7SK RNA-binding and marked decrease in 7SK RNP complex formation. Ref.6
Sequence conflict2951R → G in AAI07710. Ref.4
Sequence conflict5161K → R in AAF65503. Ref.2
Sequence conflict5161K → R in CAB43230. Ref.5
Sequence conflict5601K → Q in CAB43230. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified August 30, 2005. Version 1.
Checksum: 04209B8159A5738C

FASTA58266,899
        10         20         30         40         50         60 
METESGNQEK VMEEESTEKK KEVEKKKRSR VKQVLADIAK QVDFWFGDAN LHKDRFLREQ 

        70         80         90        100        110        120 
IEKSRDGYVD ISLLVSFNKM KKLTTDGKLI ARALRSSAVV ELDLEGTRIR RKKPLGERPK 

       130        140        150        160        170        180 
DEDERTVYVE LLPKNVNHSW IERVFGKCGN VVYISIPHYK STGDPKGFAF VEFETKEQAA 

       190        200        210        220        230        240 
KAIEFLNNPP EEAPRKPGIF PKTVKNKPIP ALRVVEEKKK KKKKKGRMKK EDNIQAKEEN 

       250        260        270        280        290        300 
MDTSNTSISK MKRSRPTSEG SDIESTEPQK QCSKKKKKRD RVEASSLPEV RTGKRKRSSS 

       310        320        330        340        350        360 
EDAESLAPRS KVKKIIQKDI IKEASEASKE NRDIEISTEE EKDTGDLKDS SLLKTKRKHK 

       370        380        390        400        410        420 
KKHKERHKMG EEVIPLRVLS KSEWMDLKKE YLALQKASMA SLKKTISQIK SESEMETDSG 

       430        440        450        460        470        480 
VPQNTGMKNE KTANREECRT QEKVNATGPQ FVSGVIVKII STEPLPGRKQ VRDTLAAISE 

       490        500        510        520        530        540 
VLYVDLLEGD TECHARFKTP EDAQAVINAY TEINKKHCWK LEILSGDHEQ RYWQKILVDR 

       550        560        570        580 
QAKLNQPREK KRGTEKLITK AEKIRLAKTQ QASKHIRFSE YD 

« Hide

Isoform 2.

Checksum: 12A03DED56668BA7
Show »

FASTA21424,488

References

« Hide 'large scale' references
[1]"The La-related protein LARP7 is a component of the 7SK ribonucleoprotein and affects transcription of cellular and viral polymerase II genes."
Markert A., Grimm M., Martinez J., Wiesner J., Meyerhans A., Meyuhas O., Sickmann A., Fischer U.
EMBO Rep. 9:569-575(2008) [PubMed: 18483487] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN THE 7SK SNRNP COMPLEX.
[2]"A novel gene from human dendritic cell."
Zhao Z., Huang X., Li N., Zhu X., Cao X.
Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Dendritic cell.
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Testis.
[5]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Blocker H., Heubner D., Hoerlein A., Michel G., Wedler H., Kohrer K., Ottenwalder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 293-582 (ISOFORM 1).
Tissue: Fetal brain.
[6]"A La-related protein modulates 7SK snRNP integrity to suppress P-TEFb-dependent transcriptional elongation and tumorigenesis."
He N., Jahchan N.S., Hong E., Li Q., Bayfield M.A., Maraia R.J., Luo K., Zhou Q.
Mol. Cell 29:588-599(2008) [PubMed: 18249148] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF TYR-128.
[7]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed: 17081983] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-337 AND THR-338, MASS SPECTROMETRY.
Tissue: Epithelium.
[8]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-257; SER-258; SER-261; SER-273; SER-337 AND THR-338, MASS SPECTROMETRY.
[9]"Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography."
Han G., Ye M., Zhou H., Jiang X., Feng S., Jiang X., Tian R., Wan D., Zou H., Gu J.
Proteomics 8:1346-1361(2008) [PubMed: 18318008] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-337 AND THR-338, MASS SPECTROMETRY.
Tissue: Liver.
[10]Colinge J., Superti-Furga G., Bennett K.L.
Submitted (OCT-2008) to UniProtKB
Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EU667388 mRNA. Translation: ACD13786.1. Different initiation.
AF068284 mRNA. Translation: AAF65503.1.
CH471057 Genomic DNA. Translation: EAX06284.1. Different initiation.
BC066945 mRNA. Translation: AAH66945.1.
BC107709 mRNA. Translation: AAI07710.1. Different initiation.
AL049996 mRNA. Translation: CAB43230.1.
IPIIPI00294742.
IPI00395536.
PIRT08692.
RefSeqNP_056269.1.
NP_057732.2.
UniGeneHs.713663

3D structure databases

ModBaseSearch...

PTM databases

PhosphoSiteQ4G0J3.

Proteomic databases

PRIDEQ4G0J3.

Genome annotation databases

EnsemblENSG00000174720. Homo sapiens. [Contig view]
GeneID51574.
KEGGhsa:51574.
UCSCuc003iay.1. human.

Organism-specific databases

GeneCardsGC04P113777.
HGNCHGNC:24912. LARP7.
HPAHPA017600.
MIM612026. gene.
GenAtlasSearch...

Phylogenomic databases

HOVERGENQ4G0J3.
OMAQ4G0J3. AKLNQPR.

Gene expression databases

ArrayExpressQ4G0J3.
BgeeQ4G0J3.
CleanExHS_LARP7.

Family and domain databases

InterProIPR012677. a_b_plait_nuc_bd.
IPR002344. Lupus_La.
IPR006630. Lupus_La_RNA_bd.
IPR014886. RRM_3.
IPR000504. RRM_RNP1.
IPR011991. Wing_hlx_DNA_bd.
[Graphical view]
Gene3DG3DSA:3.30.70.330. a_b_plait_nuc_bd. 1 hit.
G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
PfamPF05383. La. 1 hit.
PF00076. RRM_1. 1 hit.
PF08777. RRM_3. 1 hit.
[Graphical view]
PRINTSPR00302. LUPUSLA.
SMARTSM00715. LA. 1 hit.
SM00360. RRM. 1 hit.
[Graphical view]
PROSITEPS50961. HTH_LA. 1 hit.
PS50102. RRM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio55403.
SOURCESearch...

Entry information

Entry nameLARP7_HUMAN
AccessionPrimary (citable) accession number: Q4G0J3
Secondary accession number(s): B2ZHN6 expand/collapse secondary AC list , Q3B7A9, Q9P1S7, Q9Y3Z8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: August 30, 2005
Last modified: July 7, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents