Reviewed,
UniProtKB/Swiss-Prot Q4G0J3 (LARP7_HUMAN)
Last modified
February 9, 2010.
Version 53.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: La-related protein 7 Alternative name(s): La ribonucleoprotein domain family member 7 P-TEFb-interaction protein for 7SK stability Short name=PIP7S | ||||
| Gene names |
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| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 582 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Negative transcriptional regulator of polymerase II genes, acting by means of the 7SK RNP system. Within the 7SK RNP complex, the positive transcription elongation factor b (P-TEFb) is sequestered in an inactive form, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. Ref.1 Ref.6 |
| Subunit structure | Integral part of the 7SK RNP complex. Specifically binds to the highly conserved 3'-terminal U-rich stretch of 7SK RNA. On stimulation, remains associated with 7SK RNA, whereas P-TEFb is released from the complex. |
| Subcellular location | |
| Post-translational modification | Phosphorylated upon DNA damage, probably by ATM or ATR By similarity. Ref.7 Ref.8 Ref.9 Ref.11 Ref.13 |
| Sequence similarities | Contains 1 HTH La-type RNA-binding domain. Contains 1 RRM (RNA recognition motif) domain. |
| Sequence caution | The sequence ACD13786.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence EAX06284.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Nucleus |
| Coding sequence diversity | Alternative splicing |
| Ligand | RNA-binding |
| PTM | Acetylation Phosphoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | RNA processing Inferred from electronic annotation. Source: InterPro |
| Cellular component | Golgi apparatus Inferred from direct assay. Source: HPA nucleoplasmInferred from electronic annotation. Source: UniProtKB-SubCell ribonucleoprotein complexInferred from electronic annotation. Source: InterPro |
| Molecular function | RNA binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: Q4G0J3-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: Q4G0J3-2) The sequence of this isoform differs from the canonical sequence as follows: 1-368: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 582 | 582 | La-related protein 7 | PRO_0000281143 | |||||
Regions | |||||||||
| Domain | 28 – 122 | 95 | HTH La-type RNA-binding | ||||||
| Domain | 125 – 203 | 79 | RRM | ||||||
| Compositional bias | 202 – 368 | 167 | Lys-rich | ||||||
Amino acid modifications | |||||||||
| Modified residue | 254 | 1 | Phosphoserine Ref.11 | ||||||
| Modified residue | 257 | 1 | Phosphothreonine Ref.8 Ref.11 | ||||||
| Modified residue | 258 | 1 | Phosphoserine Ref.8 Ref.11 | ||||||
| Modified residue | 261 | 1 | Phosphoserine Ref.8 Ref.11 | ||||||
| Modified residue | 265 | 1 | Phosphoserine Ref.11 | ||||||
| Modified residue | 273 | 1 | Phosphoserine Ref.8 | ||||||
| Modified residue | 298 | 1 | Phosphoserine | ||||||
| Modified residue | 300 | 1 | Phosphoserine Ref.11 | ||||||
| Modified residue | 337 | 1 | Phosphoserine Ref.7 Ref.8 Ref.9 Ref.13 | ||||||
| Modified residue | 338 | 1 | Phosphothreonine Ref.7 Ref.8 Ref.9 Ref.13 | ||||||
| Modified residue | 440 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 574 | 1 | N6-acetyllysine Ref.14 | ||||||
Natural variations | |||||||||
| Alternative sequence | 1 – 368 | 368 | Missing in isoform 2. | VSP_024021 | |||||
Experimental info | |||||||||
| Mutagenesis | 128 | 1 | Y → D: Loss of 7SK RNA-binding and marked decrease in 7SK RNP complex formation. Ref.6 | ||||||
| Sequence conflict | 295 | 1 | R → G in AAI07710. Ref.4 | ||||||
| Sequence conflict | 516 | 1 | K → R in AAF65503. Ref.2 | ||||||
| Sequence conflict | 516 | 1 | K → R in CAB43230. Ref.5 | ||||||
| Sequence conflict | 560 | 1 | K → Q in CAB43230. Ref.5 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The La-related protein LARP7 is a component of the 7SK ribonucleoprotein and affects transcription of cellular and viral polymerase II genes." Markert A., Grimm M., Martinez J., Wiesner J., Meyerhans A., Meyuhas O., Sickmann A., Fischer U. EMBO Rep. 9:569-575(2008) [PubMed: 18483487] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN THE 7SK SNRNP COMPLEX. |
| [2] | "A novel gene from human dendritic cell." Zhao Z., Huang X., Li N., Zhu X., Cao X. Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Dendritic cell. |
| [3] | Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis. |
| [5] | "The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 293-582 (ISOFORM 1). Tissue: Fetal brain. |
| [6] | "A La-related protein modulates 7SK snRNP integrity to suppress P-TEFb-dependent transcriptional elongation and tumorigenesis." He N., Jahchan N.S., Hong E., Li Q., Bayfield M.A., Maraia R.J., Luo K., Zhou Q. Mol. Cell 29:588-599(2008) [PubMed: 18249148] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF TYR-128. |
| [7] | "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-337 AND THR-338, MASS SPECTROMETRY. Tissue: Epithelium. |
| [8] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-257; SER-258; SER-261; SER-273; SER-337 AND THR-338, MASS SPECTROMETRY. |
| [9] | "Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography." Han G., Ye M., Zhou H., Jiang X., Feng S., Jiang X., Tian R., Wan D., Zou H., Gu J. Proteomics 8:1346-1361(2008) [PubMed: 18318008] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-337 AND THR-338, MASS SPECTROMETRY. Tissue: Liver. |
| [10] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| [11] | "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-254; THR-257; SER-258; SER-261; SER-265 AND SER-300, MASS SPECTROMETRY. |
| [12] | "Large-scale proteomics analysis of the human kinome." Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H. Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed: 19369195] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-298, MASS SPECTROMETRY. |
| [13] | "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-337 AND THR-338, MASS SPECTROMETRY. Tissue: T-cell. |
| [14] | "Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-574, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | EU667388 mRNA. Translation: ACD13786.1. Different initiation. AF068284 mRNA. Translation: AAF65503.1. CH471057 Genomic DNA. Translation: EAX06284.1. Different initiation. BC066945 mRNA. Translation: AAH66945.1. BC107709 mRNA. Translation: AAI07710.2. AL049996 mRNA. Translation: CAB43230.1. |
| IPI | IPI00294742. IPI00395536. |
| PIR | T08692. |
| RefSeq | NP_056269.1. NP_057732.2. |
| UniGene | Hs.713663 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1ZH5 based on UniProtKB P05455. |
| SMR | Q4G0J3. Positions 34-115, 92-190, 451-552. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q4G0J3. |
PTM databases | |
| PhosphoSite | Q4G0J3. |
Proteomic databases | |
| PRIDE | Q4G0J3. |
Genome annotation databases | |
| Ensembl | ENST00000324052; ENSP00000314311; ENSG00000174720; Homo sapiens. [Genome view] ENST00000344442; ENSP00000344950; ENSG00000174720; Homo sapiens. [Genome view] |
| GeneID | 51574. |
| KEGG | hsa:51574. |
| UCSC | uc003iay.1. human. |
Organism-specific databases | |
| CTD | 51574. |
| GeneCards | GC04P113777. |
| HGNC | HGNC:24912. LARP7. |
| HPA | HPA017600. |
| MIM | 612026. gene. |
| GenAtlas | Search... |
Phylogenomic databases | |
| eggNOG | prNOG05972. |
| HOGENOM | HBG716691. |
| HOVERGEN | Q4G0J3. |
| InParanoid | Q4G0J3. |
| OMA | EVVYVDL. |
| OrthoDB | EOG9RBT3W. |
| PhylomeDB | Q4G0J3. |
Gene expression databases | |
| ArrayExpress | Q4G0J3. |
| Bgee | Q4G0J3. |
| CleanEx | HS_LARP7. |
| Genevestigator | Q4G0J3. |
Family and domain databases | |
| InterPro | IPR012677. a_b_plait_nuc_bd. IPR002344. Lupus_La. IPR006630. Lupus_La_RNA_bd. IPR014886. RRM_3. IPR000504. RRM_RNP1. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view] |
| Gene3D | G3DSA:3.30.70.330. a_b_plait_nuc_bd. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit. |
| Pfam | PF05383. La. 1 hit. PF00076. RRM_1. 1 hit. PF08777. RRM_3. 1 hit. [Graphical view] |
| PRINTS | PR00302. LUPUSLA. |
| SMART | SM00715. LA. 1 hit. SM00360. RRM. 1 hit. [Graphical view] |
| PROSITE | PS50961. HTH_LA. 1 hit. PS50102. RRM. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 55403. |
| SOURCE | Search... |
Entry information
| Entry name | LARP7_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q4G0J3 Secondary accession number(s): B2ZHN6 Q9Y3Z8 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 4 Human chromosome 4: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


