ID   MINY4_HUMAN             Reviewed;         757 AA.
AC   Q4G0A6; Q71AZ7; Q9H6D2;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   18-MAY-2010, sequence version 2.
DT   27-MAR-2024, entry version 121.
DE   RecName: Full=Probable ubiquitin carboxyl-terminal hydrolase MINDY-4;
DE            EC=3.4.19.12;
DE   AltName: Full=Probable deubiquitinating enzyme MINDY-4;
GN   Name=MINDY4 {ECO:0000312|HGNC:HGNC:21916}; Synonyms=C7orf67, FAM188B;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Brain;
RA   Ding P., Han W., Xia D., Li X., Song Q., Zhang Y., Ma D.;
RL   Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12853948; DOI=10.1038/nature01782;
RA   Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H.,
RA   Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K.,
RA   Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A.,
RA   Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H., Sun H.,
RA   Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A.,
RA   Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P.,
RA   Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M.,
RA   Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S.,
RA   Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R.,
RA   Strowmatt C., Latreille P., Miller N., Johnson D., Murray J.,
RA   Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W.,
RA   Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E.,
RA   Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A.,
RA   Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E.,
RA   Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E.,
RA   Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A.,
RA   Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A.,
RA   Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R.,
RA   McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H.,
RA   Wilson R.K.;
RT   "The DNA sequence of human chromosome 7.";
RL   Nature 424:157-164(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT MET-322.
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT MET-322.
RC   TISSUE=Chondrosarcoma, and Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-451, AND VARIANT MET-322.
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-219; SER-223 AND SER-289, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Erythroleukemia;
RX   PubMed=23186163; DOI=10.1021/pr300630k;
RA   Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA   Mohammed S.;
RT   "Toward a comprehensive characterization of a human cancer cell
RT   phosphoproteome.";
RL   J. Proteome Res. 12:260-271(2013).
CC   -!- FUNCTION: Probable hydrolase that can remove 'Lys-48'-linked conjugated
CC       ubiquitin from proteins. {ECO:0000250|UniProtKB:Q8NBR6}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide
CC         and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-
CC         residue protein attached to proteins as an intracellular targeting
CC         signal).; EC=3.4.19.12; Evidence={ECO:0000250|UniProtKB:Q8NBR6};
CC   -!- SIMILARITY: Belongs to the MINDY deubiquitinase family. FAM188
CC       subfamily. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAQ10898.1; Type=Frameshift; Evidence={ECO:0000305};
CC       Sequence=BAB15327.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence.; Evidence={ECO:0000305};
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DR   EMBL; AF538962; AAQ10898.1; ALT_FRAME; mRNA.
DR   EMBL; AC004691; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH471073; EAW93970.1; -; Genomic_DNA.
DR   EMBL; BC098569; AAH98569.1; -; mRNA.
DR   EMBL; AK026027; BAB15327.1; ALT_SEQ; mRNA.
DR   CCDS; CCDS43565.1; -.
DR   RefSeq; NP_115598.2; NM_032222.2.
DR   AlphaFoldDB; Q4G0A6; -.
DR   BioGRID; 123932; 71.
DR   STRING; 9606.ENSP00000265299; -.
DR   iPTMnet; Q4G0A6; -.
DR   PhosphoSitePlus; Q4G0A6; -.
DR   BioMuta; MINDY4; -.
DR   DMDM; 296434499; -.
DR   EPD; Q4G0A6; -.
DR   jPOST; Q4G0A6; -.
DR   MassIVE; Q4G0A6; -.
DR   MaxQB; Q4G0A6; -.
DR   PaxDb; 9606-ENSP00000265299; -.
DR   PeptideAtlas; Q4G0A6; -.
DR   ProteomicsDB; 62101; -.
DR   Pumba; Q4G0A6; -.
DR   Antibodypedia; 3440; 53 antibodies from 14 providers.
DR   DNASU; 84182; -.
DR   Ensembl; ENST00000265299.6; ENSP00000265299.6; ENSG00000106125.14.
DR   GeneID; 84182; -.
DR   KEGG; hsa:84182; -.
DR   MANE-Select; ENST00000265299.6; ENSP00000265299.6; NM_032222.3; NP_115598.2.
DR   UCSC; uc003tbt.3; human.
DR   AGR; HGNC:21916; -.
DR   DisGeNET; 84182; -.
DR   GeneCards; MINDY4; -.
DR   HGNC; HGNC:21916; MINDY4.
DR   HPA; ENSG00000106125; Low tissue specificity.
DR   neXtProt; NX_Q4G0A6; -.
DR   OpenTargets; ENSG00000106125; -.
DR   PharmGKB; PA165618038; -.
DR   VEuPathDB; HostDB:ENSG00000106125; -.
DR   eggNOG; KOG2871; Eukaryota.
DR   GeneTree; ENSGT00940000159600; -.
DR   HOGENOM; CLU_011769_1_0_1; -.
DR   InParanoid; Q4G0A6; -.
DR   OMA; NCFSVEW; -.
DR   OrthoDB; 72090at2759; -.
DR   PhylomeDB; Q4G0A6; -.
DR   TreeFam; TF323996; -.
DR   PathwayCommons; Q4G0A6; -.
DR   BioGRID-ORCS; 84182; 12 hits in 1187 CRISPR screens.
DR   GeneWiki; C7orf67; -.
DR   GenomeRNAi; 84182; -.
DR   Pharos; Q4G0A6; Tdark.
DR   PRO; PR:Q4G0A6; -.
DR   Proteomes; UP000005640; Chromosome 7.
DR   RNAct; Q4G0A6; Protein.
DR   Bgee; ENSG00000106125; Expressed in right uterine tube and 100 other cell types or tissues.
DR   GO; GO:0004843; F:cysteine-type deubiquitinase activity; IEA:UniProtKB-EC.
DR   GO; GO:1990380; F:K48-linked deubiquitinase activity; IBA:GO_Central.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   InterPro; IPR025257; MINDY-3/4_CD.
DR   InterPro; IPR039785; MINY3/4.
DR   PANTHER; PTHR12473:SF13; UBIQUITIN CARBOXYL-TERMINAL HYDROLASE MINDY-4-RELATED; 1.
DR   PANTHER; PTHR12473; UNCHARACTERIZED; 1.
DR   Pfam; PF13898; MINDY-3_4_CD; 1.
DR   SMART; SM01174; DUF4205; 1.
DR   Genevisible; Q4G0A6; HS.
PE   1: Evidence at protein level;
KW   Hydrolase; Phosphoprotein; Protease; Reference proteome; Thiol protease;
KW   Ubl conjugation pathway.
FT   CHAIN           1..757
FT                   /note="Probable ubiquitin carboxyl-terminal hydrolase
FT                   MINDY-4"
FT                   /id="PRO_0000320590"
FT   REGION          152..173
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          190..334
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        230..245
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        256..292
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        298..318
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        456
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:Q8N5J2"
FT   ACT_SITE        677
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:Q8N5J2"
FT   MOD_RES         219
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:23186163"
FT   MOD_RES         223
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:23186163"
FT   MOD_RES         289
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:23186163"
FT   VARIANT         183
FT                   /note="D -> A (in dbSNP:rs34357272)"
FT                   /id="VAR_039212"
FT   VARIANT         262
FT                   /note="S -> L (in dbSNP:rs17159453)"
FT                   /id="VAR_039213"
FT   VARIANT         322
FT                   /note="T -> M (in dbSNP:rs12701034)"
FT                   /evidence="ECO:0000269|PubMed:14702039,
FT                   ECO:0000269|PubMed:15489334, ECO:0000269|Ref.3"
FT                   /id="VAR_039214"
FT   VARIANT         324
FT                   /note="R -> K (in dbSNP:rs35897481)"
FT                   /id="VAR_039215"
FT   VARIANT         355
FT                   /note="A -> V (in dbSNP:rs12672119)"
FT                   /id="VAR_039216"
FT   VARIANT         465
FT                   /note="C -> Y (in dbSNP:rs34970833)"
FT                   /id="VAR_062248"
FT   VARIANT         655
FT                   /note="M -> V (in dbSNP:rs10216063)"
FT                   /id="VAR_039217"
FT   CONFLICT        246
FT                   /note="R -> G (in Ref. 1; AAQ10898)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        483
FT                   /note="G -> A (in Ref. 1; AAQ10898)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        632
FT                   /note="I -> F (in Ref. 1; AAQ10898)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        647
FT                   /note="L -> F (in Ref. 1; AAQ10898)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   757 AA;  84372 MW;  1DAB6CAE92042C8E CRC64;
     MDSLFVEEVA ASLVREFLSR KGLKKTCVTM DQERPRSDLS INNRNDLRKV LHLEFLYKEN
     KAKENPLKTS LELITRYFLD HFGNTANNFT QDTPIPALSV PKKNNKVPSR CSETTLVNIY
     DLSDEDAGWR TSLSETSKAR HDNLDGDVLG NFVSSKRPPH KSKPMQTVPG ETPVLTSAWE
     KIDKLHSEPS LDVKRMGENS RPKSGLIVRG MMSGPIASSP QDSFHRHYLR RSSPSSSSTQ
     PQEESRKVPE LFVCTQQDIL ASSNSSPSRT SLGQLSELTV ERQKTTASSP PHLPSKRLPP
     WDRARPRDPS EDTPAVDGST DTDRMPLKLY LPGGNSRMTQ ERLERAFKRQ GSQPAPVRKN
     QLLPSDKVDG ELGALRLEDV EDELIREEVI LSPVPSVLKL QTASKPIDLS VAKEIKTLLF
     GSSFCCFNEE WKLQSFSFSN TASLKYGIVQ NKGGPCGVLA AVQGCVLQKL LFEGDSKADC
     AQGLQPSDAH RTRCLVLALA DIVWRAGGRE RAVVALASRT QQFSPTGKYK ADGVLETLTL
     HSLTCYEDLV TFLQQSIHQF EVGPYGCILL TLSAILSRST ELIRQDFDVP TSHLIGAHGY
     CTQELVNLLL TGKAVSNVFN DVVELDSGDG NITLLRGIAA RSDIGFLSLF EHYNMCQVGC
     FLKTPRFPIW VVCSESHFSI LFSLQPGLLR DWRTERLFDL YYYDGLANQQ EQIRLTIDTT
     QTISEDTDND LVPPLELCIR TKWKGASVNW NGSDPIL
//