Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

MICAL C-terminal-like protein

Gene

Micalcl

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May cooperate with MAPK1/ERK2 via an intracellular signal transduction pathway in the morphogenetic development of round spermatids to spermatozoa.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Spermatogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
MICAL C-terminal-like protein
Alternative name(s):
ERK2-binding testicular protein 1
Ebitein-1
Protein RSB-11-77
Gene namesi
Name:Micalcl
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi735042. Micalcl.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 687687MICAL C-terminal-like proteinPRO_0000284125Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei440 – 4401PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ4G091.
PRIDEiQ4G091.

PTM databases

iPTMnetiQ4G091.

Expressioni

Gene expression databases

ExpressionAtlasiQ4G091. baseline and differential.
GenevisibleiQ4G091. RN.

Interactioni

Subunit structurei

Interacts with MAPK1/ERK2.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000039759.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni63 – 10240Interaction with MAPK1By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili515 – 56147Sequence analysisAdd
BLAST
Coiled coili587 – 66377Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi15 – 4228Ser-richAdd
BLAST
Compositional biasi515 – 5195Poly-Arg

Sequence similaritiesi

Belongs to the ebitein family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IJMF. Eukaryota.
ENOG410YJPN. LUCA.
GeneTreeiENSGT00760000118856.
HOGENOMiHOG000113587.
HOVERGENiHBG108134.
InParanoidiQ4G091.
OMAiNRPSKVF.
OrthoDBiEOG7S21XV.
PhylomeDBiQ4G091.
TreeFamiTF336446.

Family and domain databases

InterProiIPR022735. DUF3585.
[Graphical view]
PfamiPF12130. DUF3585. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q4G091-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNQRALSPPK EPPPSSSSSS PSLPSSFSSA SVPGHTTDDS SSPQVPAYNL
60 70 80 90 100
HSPQISRDDV SPTPIYLRRA RAQGITKEIP LYLPHSPMLE STEHCLVSPD
110 120 130 140 150
GEELRSPEEI SASDGCQKAL ALGNSESTHK DSYPVSGKDP YLPNQMLALG
160 170 180 190 200
AAGNTGDLSE ESRMGQTGGA ELSKERKLGL KKLVLTEEQK TMLLDWNDYT
210 220 230 240 250
QEHKAGERLA QEKAENGRGN SLKPICSSTL SQAVKEKLLS QKKALGETRT
260 270 280 290 300
PAAKAPRERE VPPPKSPLRL IANAIFRSLL PSSEAGKKTS SKPETKTLPR
310 320 330 340 350
GQPHAFTRSF SFRKLGSSKD GDQQSPGRHM AKKASAFFSL ASPTSKAAQA
360 370 380 390 400
SDLSPPNPIL RSRSLPNRPS KMFFATTSLP PSSKVEDVPT LLEKVSLQDA
410 420 430 440 450
AQGPKKGASH ISPLGLKDKS FESFLQECKE RKDIGDFFNS PKEKGPPGNR
460 470 480 490 500
VPSLEKLVQP VDSTSMGQVA HPSSTGQDAR PGAPVTEDTS SPTSSSAEED
510 520 530 540 550
VETQLSSRLK EKIPRRRRKL EKQMAKQEEL KRLHKAQAIQ RQLEEVEERQ
560 570 580 590 600
RTSEIQGVRL EKVLRGETDS GTQDEAQLLQ EWFKLVLEKN KLMRYESELL
610 620 630 640 650
IMAQELELED HQSRLEQKLR QKMLKDEGQK DENDLKEEQE IFEEMMQVIE
660 670 680
QRNKLVDSLE EQRIKERTQD QHFENFVLSR GCQLSRT
Length:687
Mass (Da):76,257
Last modified:August 30, 2005 - v1
Checksum:i421A552747EDCF38
GO
Isoform 2 (identifier: Q4G091-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-145: Missing.
     672-687: HFENFVLSRGCQLSRT → LF

Note: It is unsure whether Met-1 is the initiator methionine.
Show »
Length:528
Mass (Da):59,136
Checksum:iA3EA586775E45455
GO

Sequence cautioni

The sequence AAI00138.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAN46735.1 differs from that shown. Reason: Frameshift at position 364. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti210 – 2101A → S in AAN46735 (Ref. 1) Curated
Sequence conflicti285 – 2851A → V in AAN46735 (Ref. 1) Curated
Sequence conflicti292 – 2921K → N in AAN46735 (Ref. 1) Curated
Sequence conflicti295 – 2951T → A in AAN46735 (Ref. 1) Curated
Sequence conflicti348 – 3481A → T in AAN46735 (Ref. 1) Curated
Sequence conflicti356 – 3616PNPILR → SNPFFG in AAN46735 (Ref. 1) Curated
Sequence conflicti515 – 5151R → G in AAN46735 (Ref. 1) Curated
Sequence conflicti525 – 5251A → V in AAN46735 (Ref. 1) Curated
Sequence conflicti568 – 5681T → TA in AAI00138 (PubMed:15489334).Curated
Sequence conflicti643 – 6431E → K in AAN46735 (Ref. 1) Curated
Sequence conflicti672 – 6721H → L in AAN46735 (Ref. 1) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 145145Missing in isoform 2. 1 PublicationVSP_024452Add
BLAST
Alternative sequencei672 – 68716HFENF…QLSRT → LF in isoform 2. 1 PublicationVSP_024454Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY149342 mRNA. Translation: AAN46735.1. Frameshift.
BC098647 mRNA. Translation: AAH98647.1.
BC100137 mRNA. Translation: AAI00138.1. Different initiation.
RefSeqiNP_872610.2. NM_182669.2. [Q4G091-1]
UniGeneiRn.30134.

Genome annotation databases

EnsembliENSRNOT00000043017; ENSRNOP00000039759; ENSRNOG00000016210. [Q4G091-1]
GeneIDi293180.
KEGGirno:293180.
UCSCiRGD:735042. rat. [Q4G091-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY149342 mRNA. Translation: AAN46735.1. Frameshift.
BC098647 mRNA. Translation: AAH98647.1.
BC100137 mRNA. Translation: AAI00138.1. Different initiation.
RefSeqiNP_872610.2. NM_182669.2. [Q4G091-1]
UniGeneiRn.30134.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000039759.

PTM databases

iPTMnetiQ4G091.

Proteomic databases

PaxDbiQ4G091.
PRIDEiQ4G091.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000043017; ENSRNOP00000039759; ENSRNOG00000016210. [Q4G091-1]
GeneIDi293180.
KEGGirno:293180.
UCSCiRGD:735042. rat. [Q4G091-1]

Organism-specific databases

CTDi84953.
RGDi735042. Micalcl.

Phylogenomic databases

eggNOGiENOG410IJMF. Eukaryota.
ENOG410YJPN. LUCA.
GeneTreeiENSGT00760000118856.
HOGENOMiHOG000113587.
HOVERGENiHBG108134.
InParanoidiQ4G091.
OMAiNRPSKVF.
OrthoDBiEOG7S21XV.
PhylomeDBiQ4G091.
TreeFamiTF336446.

Miscellaneous databases

PROiQ4G091.

Gene expression databases

ExpressionAtlasiQ4G091. baseline and differential.
GenevisibleiQ4G091. RN.

Family and domain databases

InterProiIPR022735. DUF3585.
[Graphical view]
PfamiPF12130. DUF3585. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A new gene from rat testis cDNA library."
    Liang G., Miao S.Y., Zhang X.D., Wang L.J., Wang L.F.
    Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Testis.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-440, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMICLK_RAT
AccessioniPrimary (citable) accession number: Q4G091
Secondary accession number(s): Q498N8, Q80WN5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: August 30, 2005
Last modified: June 8, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.