Reviewed, UniProtKB/Swiss-Prot Q4G073 (TRDMT_RAT)

Names and origin

Protein names

Recommended name:
    tRNA (cytosine-5-)-methyltransferase
    EC=2.1.1.29
Alternative name(s):
    DNA (cytosine-5)-methyltransferase-like protein 2
      Short name=Dnmt2

Gene names

Name:	Trdmt1
Synonyms:	Dnmt2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	391 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp) By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing 5-methylcytosine.
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro response to amphetamine Inferred from expression pattern. Source: RGD tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 391	391	tRNA (cytosine-5-)-methyltransferase		PRO_0000249870
Sites
	Active site	79	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4G073-1 [UniParc]. Last modified August 30, 2005. Version 1. Checksum: 58768BA5BBD4E37C	FASTA	391	44,070
	10 20 30 40 50 60 MEPLRVLELY SGIGGMHHAL RESRVPAHVV AAIDVSTVAN EVYKHNFPHT HLLAKTIEGI 70 80 90 100 110 120 SLEEFDKLSF NMILMSPPCQ PFTRIGLQGD MSDRRTNSFL YILDILPRLQ KLPKYILLEN 130 140 150 160 170 180 VKGFEVSSTR GLLIQTMEAC GFQYQEFLLS PSSLGIPNSR LRYFLIAKLQ SEPLCFQAPG 190 200 210 220 230 240 QILMEFPNSG TVQPQEYAVV EEGKLRVRTR EPDVCLDSSS TQCSGQDSIL FKHETAADID 250 260 270 280 290 300 RKRQQDSDLS VQMLKGFLED GDTAQYLLPA KSLLRYALLL DIVKPTSRRS MCFTKGYGSY 310 320 330 340 350 360 IEGTGSVLQT AEDVQIENIY KSLPDLPPEE KIAKLSMLKL RYFTPKEIAN LLGFPPEFGF 370 380 390 PEKTTVKQRY RLLGNSLNVH VVSKLLTVLC E

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Thymus.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC098700 mRNA. Translation: AAH98700.1.
IPI	IPI00363164.
RefSeq	NP_001026813.1.
UniGene	Rn.115
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000026132. Rattus norvegicus. [Contig view]
GeneID	291324.
KEGG	rno:291324.
NMPDR	fig|10116.3.peg.13667.
Organism-specific databases
RGD	1306292. Trdmt1.
Phylogenomic databases
HOVERGEN	Q4G073.
OMA	Q4G073. YFTPREV.
Enzyme and pathway databases
BRENDA	2.1.1.29. 248.
Gene expression databases
ArrayExpress	Q4G073.
GermOnline	ENSRNOG00000026132. Rattus norvegicus.
Family and domain databases
InterPro	IPR001525. C5_DNA_meth. IPR018117. C5_DNA_meth_AS. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF00145. DNA_methylase. 2 hits. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
PROSITE	PS00094. C5_MTASE_1. False negative. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	632414.

Entry information

Entry name

TRDMT_RAT

Accession

Primary (citable) accession number: Q4G073

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
Last sequence update:	August 30, 2005
Last modified:	June 16, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)