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Protein

Eukaryotic translation initiation factor 3 subunit B

Gene

Eif3b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-RNO-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-RNO-72649. Translation initiation complex formation.
R-RNO-72689. Formation of a pool of free 40S subunits.
R-RNO-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-RNO-72702. Ribosomal scanning and start codon recognition.
R-RNO-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit BUniRule annotation
Short name:
eIF3bUniRule annotation
Alternative name(s):
Eukaryotic translation initiation factor 3 subunit 9UniRule annotation
eIF-3-etaUniRule annotation
Gene namesi
Name:Eif3b
Synonyms:Eif3s9
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi1309018. Eif3b.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 797797Eukaryotic translation initiation factor 3 subunit BPRO_0000223480Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineUniRule annotationBy similarity
Modified residuei31 – 311PhosphothreonineBy similarity
Modified residuei37 – 371PhosphoserineCombined sources
Modified residuei40 – 401PhosphoserineCombined sources
Modified residuei68 – 681PhosphoserineUniRule annotationBy similarity
Modified residuei72 – 721PhosphothreonineBy similarity
Modified residuei75 – 751PhosphoserineCombined sources
Modified residuei79 – 791PhosphoserineCombined sources
Modified residuei84 – 841PhosphoserineBy similarity
Modified residuei105 – 1051PhosphoserineBy similarity
Modified residuei114 – 1141PhosphoserineCombined sources
Modified residuei117 – 1171PhosphoserineCombined sources
Modified residuei135 – 1351PhosphoserineCombined sources
Modified residuei137 – 1371PhosphoserineCombined sources
Modified residuei147 – 1471PhosphoserineCombined sources
Modified residuei192 – 1921N6-acetyllysineBy similarity
Modified residuei222 – 2221PhosphoserineUniRule annotationBy similarity
Modified residuei271 – 2711N6-acetyllysineBy similarity
Modified residuei347 – 3471N6-acetyllysineBy similarity

Post-translational modificationi

Phosphorylated. Phosphorylation is enhanced upon serum stimulation.UniRule annotation

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ4G061.
PRIDEiQ4G061.

PTM databases

iPTMnetiQ4G061.
PhosphoSiteiQ4G061.

Expressioni

Gene expression databases

BgeeiENSRNOG00000001253.
GenevisibleiQ4G061. RN.

Interactioni

Subunit structurei

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Also interacts with UPF2 and HNRPD. Interacts with METTL3.UniRule annotation

Protein-protein interaction databases

BioGridi252584. 1 interaction.
IntActiQ4G061. 2 interactions.
STRINGi10116.ENSRNOP00000057188.

Structurei

3D structure databases

ProteinModelPortaliQ4G061.
SMRiQ4G061. Positions 153-257.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini168 – 25184RRMUniRule annotationAdd
BLAST
Repeati315 – 35339WD 1Add
BLAST
Repeati355 – 40046WD 2Add
BLAST
Repeati404 – 44239WD 3Add
BLAST
Repeati543 – 58442WD 4Add
BLAST
Repeati632 – 67746WD 5Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni107 – 396290Sufficient for interaction with EIF3EUniRule annotationAdd
BLAST
Regioni153 – 257105Sufficient for interaction with EIF3JUniRule annotationAdd
BLAST

Domaini

The RRM domain mediates interaction with EIF3J.UniRule annotation

Sequence similaritiesi

Belongs to the eIF-3 subunit B family.UniRule annotation
Contains 1 RRM (RNA recognition motif) domain.UniRule annotation
Contains 5 WD repeats.UniRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG2314. Eukaryota.
COG5354. LUCA.
GeneTreeiENSGT00550000074913.
HOGENOMiHOG000265546.
HOVERGENiHBG006127.
InParanoidiQ4G061.
KOiK03253.
OMAiAFMEYKQ.
OrthoDBiEOG091G02V5.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
2.130.10.10. 1 hit.
3.30.70.330. 1 hit.
HAMAPiMF_03001. eIF3b. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR011400. EIF3B.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR013979. TIF_beta_prop-like.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PANTHERiPTHR14068. PTHR14068. 1 hit.
PfamiPF08662. eIF2A. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
PIRSFiPIRSF036424. eIF3b. 1 hit.
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4G061-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQDAENVAAP EAAEERAEPA RQQPVSESPP TDEAAGSGGS EVGRTEDAEE
60 70 80 90 100
DAEARPEPEV RAKPAAQSEE ETAASPAASP TPQSAQEPSA PGKAEAGGEQ
110 120 130 140 150
ARHPSARAEE EGGSDGSAAE AEPRALENGE ADEPSFSDPE DFVDDVSEEE
160 170 180 190 200
LLGDVLKDRP QEADGIDSVI VVDNVPQVGP DRLEKLKNVI HKIFSKFGKI
210 220 230 240 250
INDYYPEEDG KTKGYIFLEY ASPAHAVDAV KNADGYKLDK QHTFRVNLFT
260 270 280 290 300
DFDKYMTISD EWDIPEKQPF KDLGNLRYWL EEAECRDQYS VIFESGDRTS
310 320 330 340 350
IFWNDVKDPV SIEERARWTE TYVRWSPKGT YLATFHQRGI ALWGGDKFKQ
360 370 380 390 400
IQRFSHQGVQ LIDFSPCERY LVTFSPLMDT QDDPQAIIIW DILTGHKKRG
410 420 430 440 450
FHCESSAHWP IFKWSHDGKF FARMTLDTLS IYETPSMGLL DKKSLKISGI
460 470 480 490 500
KDFSWSPGGN IIAFWVPEDK DIPARVTLMQ LPTRQEIRVR NLFNVVDCKL
510 520 530 540 550
HWQKNGDYLC VKVDRTPKGT QGVVTNFEIF RMREKQVPVD VVEMKETIIA
560 570 580 590 600
FAWEPNGSKF AVLHGEAPRI SVSFYHVKSN GKIELIKMFD KQQANTIFWS
610 620 630 640 650
PQGQFVVLAG LRSMNGALAF VDTSDCTVMN IAEHYMASDV EWDPTGRYVV
660 670 680 690 700
TSVSWWSHKV DNAYWLWTFQ GRLLQKNNKD RFCQLLWRPR PPTLLSQDQI
710 720 730 740 750
KQIKKDLKKY SKIFEQKDRL SQSKASKELV ERRRTMMEDF RQYRKMAQEL
760 770 780 790
YMKQKNERLE LRGGVDTDEL DSNVDDWEEE TIEFFVTEEV IPLGSQE
Length:797
Mass (Da):90,911
Last modified:August 30, 2005 - v1
Checksum:iDACAA7CABE41EBA2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC098728 mRNA. Translation: AAH98728.1.
RefSeqiNP_001026810.1. NM_001031640.1.
UniGeneiRn.2829.

Genome annotation databases

EnsembliENSRNOT00000060444; ENSRNOP00000057188; ENSRNOG00000001253.
GeneIDi288516.
KEGGirno:288516.
UCSCiRGD:1309018. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC098728 mRNA. Translation: AAH98728.1.
RefSeqiNP_001026810.1. NM_001031640.1.
UniGeneiRn.2829.

3D structure databases

ProteinModelPortaliQ4G061.
SMRiQ4G061. Positions 153-257.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi252584. 1 interaction.
IntActiQ4G061. 2 interactions.
STRINGi10116.ENSRNOP00000057188.

PTM databases

iPTMnetiQ4G061.
PhosphoSiteiQ4G061.

Proteomic databases

PaxDbiQ4G061.
PRIDEiQ4G061.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000060444; ENSRNOP00000057188; ENSRNOG00000001253.
GeneIDi288516.
KEGGirno:288516.
UCSCiRGD:1309018. rat.

Organism-specific databases

CTDi8662.
RGDi1309018. Eif3b.

Phylogenomic databases

eggNOGiKOG2314. Eukaryota.
COG5354. LUCA.
GeneTreeiENSGT00550000074913.
HOGENOMiHOG000265546.
HOVERGENiHBG006127.
InParanoidiQ4G061.
KOiK03253.
OMAiAFMEYKQ.
OrthoDBiEOG091G02V5.

Enzyme and pathway databases

ReactomeiR-RNO-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-RNO-72649. Translation initiation complex formation.
R-RNO-72689. Formation of a pool of free 40S subunits.
R-RNO-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-RNO-72702. Ribosomal scanning and start codon recognition.
R-RNO-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.

Miscellaneous databases

PROiQ4G061.

Gene expression databases

BgeeiENSRNOG00000001253.
GenevisibleiQ4G061. RN.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
2.130.10.10. 1 hit.
3.30.70.330. 1 hit.
HAMAPiMF_03001. eIF3b. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR011400. EIF3B.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR013979. TIF_beta_prop-like.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PANTHERiPTHR14068. PTHR14068. 1 hit.
PfamiPF08662. eIF2A. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
PIRSFiPIRSF036424. eIF3b. 1 hit.
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEIF3B_RAT
AccessioniPrimary (citable) accession number: Q4G061
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: August 30, 2005
Last modified: September 7, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.