Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

MAP kinase-interacting serine/threonine-protein kinase 1

Gene

Mknk1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Phosphorylated and activated by the p38 kinases and kinases in the Erk pathway.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei66 – 661ATPPROSITE-ProRule annotation
Active sitei158 – 1581Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi43 – 519ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • intracellular signal transduction Source: GO_Central
  • peptidyl-serine phosphorylation Source: RGD
  • protein autophosphorylation Source: GO_Central
  • regulation of translational initiation Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Translation regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-1295596. Spry regulation of FGF signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
MAP kinase-interacting serine/threonine-protein kinase 1 (EC:2.7.11.1)
Alternative name(s):
MAP kinase signal-integrating kinase 1
Short name:
MAPK signal-integrating kinase 1
Short name:
Mnk1
Gene namesi
Name:Mknk1
Synonyms:Mnk1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi1559603. Mknk1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • cytosol Source: RGD
  • nucleus Source: GO_Central
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 413413MAP kinase-interacting serine/threonine-protein kinase 1PRO_0000226969Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei22 – 221Phosphothreonine; by PAK2By similarity
Modified residuei27 – 271Phosphoserine; by PAK2By similarity
Modified residuei168 – 1681PhosphoserineBy similarity
Modified residuei173 – 1731PhosphoserineBy similarity
Modified residuei197 – 1971PhosphothreonineBy similarity
Modified residuei202 – 2021PhosphothreonineBy similarity
Modified residuei332 – 3321PhosphothreonineBy similarity

Post-translational modificationi

Dual phosphorylation of Thr-197 and Thr-202 activates the kinase. Phosphorylation of Thr-332 activates the kinase. MAPK3/ERK1 is one of the kinases which activate MKNK1/MNK1. Phosphorylation by PAK2 leads to a reduced phosphorylation of EIF4G1 (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ4G050.
PRIDEiQ4G050.

Expressioni

Gene expression databases

ExpressionAtlasiQ4G050. baseline and differential.
GenevisibleiQ4G050. RN.

Interactioni

Subunit structurei

Interacts with the C-terminal regions of EIF4G1 and EIF4G2. Also binds to dephosphorylated ERK1 and ERK2, and to the p38 kinases (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000061535.

Structurei

3D structure databases

ProteinModelPortaliQ4G050.
SMRiQ4G050. Positions 27-323.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 321285Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0607. Eukaryota.
ENOG410XQA9. LUCA.
GeneTreeiENSGT00830000128274.
HOGENOMiHOG000231140.
HOVERGENiHBG106949.
InParanoidiQ4G050.
KOiK04372.
OMAiILTHIQK.
OrthoDBiEOG7SV0VB.
PhylomeDBiQ4G050.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4G050-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSSEPLPIV DSDKRRKKKR KTRATDSLPG KFEDVYQLTS ELLGEGAYAK
60 70 80 90 100
VQGAVSLQSG KEYAVKIIEK QAGHSRSRVF REVETLYQCQ GNRNILELIE
110 120 130 140 150
FFEDDTRFYL VFEKLQGGSI LAHIQKRKHF NELEASRVVR DVATALDFLH
160 170 180 190 200
TKGIAHRDLK PENILCESPE KVSPVKICDF DLGSGVKLNN SCTPITTPEL
210 220 230 240 250
TTPCGSAEYM APEVVEVFRD EATFYDKRCD LWSLGVVLYI MLSGYPPFVG
260 270 280 290 300
HCGADCGWDR GEVCRMCQNK LFESIQEGKY EFPDKDWAHI STEAKDLISK
310 320 330 340 350
LLVRDAKQRL SAAQVLQHPW VQGQAPERGL PTPQVLQRNS STMDLTLFAA
360 370 380 390 400
EAIALNRQLS QHEENELAEE HEALAEGLCS MKLSPPSKSR LARRRALAHA
410
GREANSCSTP AGL
Length:413
Mass (Da):46,214
Last modified:August 30, 2005 - v1
Checksum:iDE0DA795C64D0161
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC098754 mRNA. Translation: AAH98754.1.
RefSeqiNP_001037732.1. NM_001044267.1.
XP_006238760.1. XM_006238698.2.
XP_006238761.1. XM_006238699.2.
UniGeneiRn.230218.

Genome annotation databases

EnsembliENSRNOT00000067655; ENSRNOP00000061535; ENSRNOG00000010381.
GeneIDi500526.
KEGGirno:500526.
UCSCiRGD:1559603. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC098754 mRNA. Translation: AAH98754.1.
RefSeqiNP_001037732.1. NM_001044267.1.
XP_006238760.1. XM_006238698.2.
XP_006238761.1. XM_006238699.2.
UniGeneiRn.230218.

3D structure databases

ProteinModelPortaliQ4G050.
SMRiQ4G050. Positions 27-323.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000061535.

Proteomic databases

PaxDbiQ4G050.
PRIDEiQ4G050.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000067655; ENSRNOP00000061535; ENSRNOG00000010381.
GeneIDi500526.
KEGGirno:500526.
UCSCiRGD:1559603. rat.

Organism-specific databases

CTDi8569.
RGDi1559603. Mknk1.

Phylogenomic databases

eggNOGiKOG0607. Eukaryota.
ENOG410XQA9. LUCA.
GeneTreeiENSGT00830000128274.
HOGENOMiHOG000231140.
HOVERGENiHBG106949.
InParanoidiQ4G050.
KOiK04372.
OMAiILTHIQK.
OrthoDBiEOG7SV0VB.
PhylomeDBiQ4G050.

Enzyme and pathway databases

ReactomeiR-RNO-1295596. Spry regulation of FGF signaling.

Miscellaneous databases

PROiQ4G050.

Gene expression databases

ExpressionAtlasiQ4G050. baseline and differential.
GenevisibleiQ4G050. RN.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.

Entry informationi

Entry nameiMKNK1_RAT
AccessioniPrimary (citable) accession number: Q4G050
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: August 30, 2005
Last modified: July 6, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.