Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q4FZV0

- MANBA_RAT

UniProt

Q4FZV0 - MANBA_RAT

Protein

Beta-mannosidase

Gene

Manba

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (30 Aug 2005)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of all N-linked glycoprotein oligosaccharides.By similarity

    Catalytic activityi

    Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei457 – 4571Proton donorBy similarity

    GO - Molecular functioni

    1. beta-mannosidase activity Source: RGD
    2. mannose binding Source: RGD

    GO - Biological processi

    1. mannan catabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    SABIO-RKQ4FZV0.
    UniPathwayiUPA00280.

    Protein family/group databases

    CAZyiGH2. Glycoside Hydrolase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-mannosidase (EC:3.2.1.25)
    Alternative name(s):
    Lysosomal beta A mannosidase
    Mannanase
    Short name:
    Mannase
    Gene namesi
    Name:ManbaImported
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 2

    Organism-specific databases

    RGDi1305785. Manba.

    Subcellular locationi

    Lysosome By similarity

    GO - Cellular componenti

    1. lysosome Source: RGD

    Keywords - Cellular componenti

    Lysosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Chaini19 – 881863Beta-mannosidaseSequence AnalysisPRO_0000250614Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi35 – 351N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi77 – 771N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi89 – 891N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi113 – 1131N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi226 – 2261N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi297 – 2971N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi302 – 3021N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi803 – 8031N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ4FZV0.
    PRIDEiQ4FZV0.

    Expressioni

    Gene expression databases

    GenevestigatoriQ4FZV0.

    Interactioni

    Structurei

    3D structure databases

    ProteinModelPortaliQ4FZV0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.Sequence Analysis

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3250.
    GeneTreeiENSGT00390000001670.
    HOGENOMiHOG000186862.
    HOVERGENiHBG052404.
    InParanoidiQ4FZV0.
    KOiK01192.
    OrthoDBiEOG7S7SD2.
    PhylomeDBiQ4FZV0.
    TreeFamiTF105723.

    Family and domain databases

    Gene3Di2.60.120.260. 2 hits.
    3.20.20.80. 2 hits.
    InterProiIPR008979. Galactose-bd-like.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR028369. Mannanase.
    [Graphical view]
    PANTHERiPTHR10066:SF12. PTHR10066:SF12. 1 hit.
    PfamiPF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 11 Publication (identifier: Q4FZV0-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MHLHLLFLLA LCGAGCMAAG PSYSLRGSWR VSNGNSSLEL PATVPGYVHS    50
    ALQQHGLIQD PYYRFNDLNY RWISLDNWTY STEFKIPFNR SEWQKVKLIF 100
    DGVDTVAEIL FNNVTIGKTD NMFTRYSFDV TNVVKDVNSL KLRFQSAVQY 150
    AECQSKAHTQ YRVPPECPPV EQKGECHVNF IRKEQCSFSW DWGPSFPSQG 200
    IWKDVRIEAY NIAHLDHLTF LPLYDNTSQA WTIEIEASFD VVSTKPVGGQ 250
    VTIAIPELKT QQANHIELQH GQRIVKLLVK IRKDVTVETW WPHGHGNQTG 300
    YNTTILFALD GGLKIEKAAK VYFRTVQLIE EPITGSPGLS FYFKINGLPI 350
    FLKGSNWIPA DSFQDKVTSE LLQLLLQSAV DANMNTLRVW GGGIYEQDEF 400
    YALCDELGIM VWQDFMFASA LYPTEPGFLE SVRKEVTYQV RRLKSHPSVI 450
    IWSGNNENEV ALRVNWFHVN PRDLGTYIND YVTLYVKTIR EIVLSEDRSR 500
    PFIASSPTNG VKTMTEGWIS KDPYSTQYGD MHFYDYFSDC WDWKVFPKAR 550
    LVSEYGYQSW PSFSTLQKVS RQEDWSYSSR FSLHRQHHGN GNNEMLHQVQ 600
    LHFQLPQRRD PVRAFKDTIY LTQVMQAQCI KTETEFYLRS RSEIVNGEGH 650
    TMGALYWQLN DIWQAPSWAS LEYGGKWKML HYFAQRFFAP LLPVGFEDEG 700
    VFYVYGVSDL HKDYPTKLTV RLHRWSSQKP LCTFVSLSAV IKAGEAMVLF 750
    QMPVSKLLKR CKECTRDTCV VSFYLSTDNE LFSPTNYHFL SSLKDAKGMV 800
    KANITVSISQ KGDLFVFDLK SSTSAITPFV WLDVGSIPGR FSDNGFLMIK 850
    KELSVLFYPW KPTSKSELQQ AFSVTSLTDL Y 881
    Length:881
    Mass (Da):101,221
    Last modified:August 30, 2005 - v1
    Checksum:i023E3BE808CE6306
    GO
    Isoform 21 Publication (identifier: Q4FZV0-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-383: Missing.

    Note: No experimental confirmation available.Curated

    Show »
    Length:498
    Mass (Da):57,806
    Checksum:iE03D5EE26E6D60CF
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 383383Missing in isoform 2. 1 PublicationVSP_052170Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC099094 mRNA. Translation: AAH99094.1.
    BC111710 mRNA. Translation: AAI11711.1.
    RefSeqiNP_001026825.1. NM_001031655.1. [Q4FZV0-1]
    UniGeneiRn.20578.

    Genome annotation databases

    EnsembliENSRNOT00000073629; ENSRNOP00000064512; ENSRNOG00000013476. [Q4FZV0-2]
    GeneIDi310864.
    KEGGirno:310864.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC099094 mRNA. Translation: AAH99094.1 .
    BC111710 mRNA. Translation: AAI11711.1 .
    RefSeqi NP_001026825.1. NM_001031655.1. [Q4FZV0-1 ]
    UniGenei Rn.20578.

    3D structure databases

    ProteinModelPortali Q4FZV0.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi Q4FZV0.
    ChEMBLi CHEMBL3431.

    Protein family/group databases

    CAZyi GH2. Glycoside Hydrolase Family 2.

    Proteomic databases

    PaxDbi Q4FZV0.
    PRIDEi Q4FZV0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000073629 ; ENSRNOP00000064512 ; ENSRNOG00000013476 . [Q4FZV0-2 ]
    GeneIDi 310864.
    KEGGi rno:310864.

    Organism-specific databases

    CTDi 4126.
    RGDi 1305785. Manba.

    Phylogenomic databases

    eggNOGi COG3250.
    GeneTreei ENSGT00390000001670.
    HOGENOMi HOG000186862.
    HOVERGENi HBG052404.
    InParanoidi Q4FZV0.
    KOi K01192.
    OrthoDBi EOG7S7SD2.
    PhylomeDBi Q4FZV0.
    TreeFami TF105723.

    Enzyme and pathway databases

    UniPathwayi UPA00280 .
    SABIO-RK Q4FZV0.

    Miscellaneous databases

    NextBioi 662767.
    PROi Q4FZV0.

    Gene expression databases

    Genevestigatori Q4FZV0.

    Family and domain databases

    Gene3Di 2.60.120.260. 2 hits.
    3.20.20.80. 2 hits.
    InterProi IPR008979. Galactose-bd-like.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR028369. Mannanase.
    [Graphical view ]
    PANTHERi PTHR10066:SF12. PTHR10066:SF12. 1 hit.
    Pfami PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Placenta and Thymus.

    Entry informationi

    Entry nameiMANBA_RAT
    AccessioniPrimary (citable) accession number: Q4FZV0
    Secondary accession number(s): Q2NKP5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 3, 2006
    Last sequence update: August 30, 2005
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3