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Protein

Beta-mannosidase

Gene

Manba

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of all N-linked glycoprotein oligosaccharides.By similarity

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei457 – 4571Proton donorBy similarity

GO - Molecular functioni

  1. beta-mannosidase activity Source: RGD
  2. mannose binding Source: RGD

GO - Biological processi

  1. carbohydrate metabolic process Source: GO_Central
  2. mannan catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

SABIO-RKQ4FZV0.
UniPathwayiUPA00280.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-mannosidase (EC:3.2.1.25)
Alternative name(s):
Lysosomal beta A mannosidase
Mannanase
Short name:
Mannase
Gene namesi
Name:ManbaImported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 2

Organism-specific databases

RGDi1305785. Manba.

Subcellular locationi

Lysosome By similarity

GO - Cellular componenti

  1. lysosome Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence AnalysisAdd
BLAST
Chaini19 – 881863Beta-mannosidaseSequence AnalysisPRO_0000250614Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi35 – 351N-linked (GlcNAc...)Sequence Analysis
Glycosylationi77 – 771N-linked (GlcNAc...)Sequence Analysis
Glycosylationi89 – 891N-linked (GlcNAc...)Sequence Analysis
Glycosylationi113 – 1131N-linked (GlcNAc...)Sequence Analysis
Glycosylationi226 – 2261N-linked (GlcNAc...)Sequence Analysis
Glycosylationi297 – 2971N-linked (GlcNAc...)Sequence Analysis
Glycosylationi302 – 3021N-linked (GlcNAc...)Sequence Analysis
Glycosylationi803 – 8031N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ4FZV0.
PRIDEiQ4FZV0.

Expressioni

Gene expression databases

ExpressionAtlasiQ4FZV0. baseline and differential.
GenevestigatoriQ4FZV0.

Interactioni

Structurei

3D structure databases

ProteinModelPortaliQ4FZV0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.Sequence Analysis

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3250.
GeneTreeiENSGT00390000001670.
HOGENOMiHOG000186862.
HOVERGENiHBG052404.
InParanoidiQ4FZV0.
KOiK01192.
OrthoDBiEOG7S7SD2.
PhylomeDBiQ4FZV0.
TreeFamiTF105723.

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
3.20.20.80. 2 hits.
InterProiIPR008979. Galactose-bd-like.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR028369. Mannanase.
[Graphical view]
PANTHERiPTHR10066:SF12. PTHR10066:SF12. 1 hit.
PfamiPF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q4FZV0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHLHLLFLLA LCGAGCMAAG PSYSLRGSWR VSNGNSSLEL PATVPGYVHS
60 70 80 90 100
ALQQHGLIQD PYYRFNDLNY RWISLDNWTY STEFKIPFNR SEWQKVKLIF
110 120 130 140 150
DGVDTVAEIL FNNVTIGKTD NMFTRYSFDV TNVVKDVNSL KLRFQSAVQY
160 170 180 190 200
AECQSKAHTQ YRVPPECPPV EQKGECHVNF IRKEQCSFSW DWGPSFPSQG
210 220 230 240 250
IWKDVRIEAY NIAHLDHLTF LPLYDNTSQA WTIEIEASFD VVSTKPVGGQ
260 270 280 290 300
VTIAIPELKT QQANHIELQH GQRIVKLLVK IRKDVTVETW WPHGHGNQTG
310 320 330 340 350
YNTTILFALD GGLKIEKAAK VYFRTVQLIE EPITGSPGLS FYFKINGLPI
360 370 380 390 400
FLKGSNWIPA DSFQDKVTSE LLQLLLQSAV DANMNTLRVW GGGIYEQDEF
410 420 430 440 450
YALCDELGIM VWQDFMFASA LYPTEPGFLE SVRKEVTYQV RRLKSHPSVI
460 470 480 490 500
IWSGNNENEV ALRVNWFHVN PRDLGTYIND YVTLYVKTIR EIVLSEDRSR
510 520 530 540 550
PFIASSPTNG VKTMTEGWIS KDPYSTQYGD MHFYDYFSDC WDWKVFPKAR
560 570 580 590 600
LVSEYGYQSW PSFSTLQKVS RQEDWSYSSR FSLHRQHHGN GNNEMLHQVQ
610 620 630 640 650
LHFQLPQRRD PVRAFKDTIY LTQVMQAQCI KTETEFYLRS RSEIVNGEGH
660 670 680 690 700
TMGALYWQLN DIWQAPSWAS LEYGGKWKML HYFAQRFFAP LLPVGFEDEG
710 720 730 740 750
VFYVYGVSDL HKDYPTKLTV RLHRWSSQKP LCTFVSLSAV IKAGEAMVLF
760 770 780 790 800
QMPVSKLLKR CKECTRDTCV VSFYLSTDNE LFSPTNYHFL SSLKDAKGMV
810 820 830 840 850
KANITVSISQ KGDLFVFDLK SSTSAITPFV WLDVGSIPGR FSDNGFLMIK
860 870 880
KELSVLFYPW KPTSKSELQQ AFSVTSLTDL Y
Length:881
Mass (Da):101,221
Last modified:August 30, 2005 - v1
Checksum:i023E3BE808CE6306
GO
Isoform 21 Publication (identifier: Q4FZV0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-383: Missing.

Note: No experimental confirmation available.Curated

Show »
Length:498
Mass (Da):57,806
Checksum:iE03D5EE26E6D60CF
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 383383Missing in isoform 2. 1 PublicationVSP_052170Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC099094 mRNA. Translation: AAH99094.1.
BC111710 mRNA. Translation: AAI11711.1.
RefSeqiNP_001026825.1. NM_001031655.1. [Q4FZV0-1]
UniGeneiRn.20578.

Genome annotation databases

EnsembliENSRNOT00000073629; ENSRNOP00000064512; ENSRNOG00000013476. [Q4FZV0-2]
GeneIDi310864.
KEGGirno:310864.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC099094 mRNA. Translation: AAH99094.1.
BC111710 mRNA. Translation: AAI11711.1.
RefSeqiNP_001026825.1. NM_001031655.1. [Q4FZV0-1]
UniGeneiRn.20578.

3D structure databases

ProteinModelPortaliQ4FZV0.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

BindingDBiQ4FZV0.
ChEMBLiCHEMBL3431.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Proteomic databases

PaxDbiQ4FZV0.
PRIDEiQ4FZV0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000073629; ENSRNOP00000064512; ENSRNOG00000013476. [Q4FZV0-2]
GeneIDi310864.
KEGGirno:310864.

Organism-specific databases

CTDi4126.
RGDi1305785. Manba.

Phylogenomic databases

eggNOGiCOG3250.
GeneTreeiENSGT00390000001670.
HOGENOMiHOG000186862.
HOVERGENiHBG052404.
InParanoidiQ4FZV0.
KOiK01192.
OrthoDBiEOG7S7SD2.
PhylomeDBiQ4FZV0.
TreeFamiTF105723.

Enzyme and pathway databases

UniPathwayiUPA00280.
SABIO-RKQ4FZV0.

Miscellaneous databases

NextBioi662767.
PROiQ4FZV0.

Gene expression databases

ExpressionAtlasiQ4FZV0. baseline and differential.
GenevestigatoriQ4FZV0.

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
3.20.20.80. 2 hits.
InterProiIPR008979. Galactose-bd-like.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR028369. Mannanase.
[Graphical view]
PANTHERiPTHR10066:SF12. PTHR10066:SF12. 1 hit.
PfamiPF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Placenta and Thymus.

Entry informationi

Entry nameiMANBA_RAT
AccessioniPrimary (citable) accession number: Q4FZV0
Secondary accession number(s): Q2NKP5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: August 30, 2005
Last modified: February 4, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.