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Q4FZV0 (MANBA_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-mannosidase
EC=3.2.1.25
Alternative name(s):
Lysosomal beta A mannosidase
Mannanase
Short name=Mannase
Gene names
Name:Manba
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length881 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of all N-linked glycoprotein oligosaccharides By similarity. UniProtKB O00462

Catalytic activity

Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides. UniProtKB O00462

Pathway

Glycan metabolism; N-glycan degradation.

Subcellular location

Lysosome By similarity UniProtKB O00462.

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

Ontologies

Keywords
   Cellular componentLysosome
   Coding sequence diversityAlternative splicing
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

glycoprotein catabolic process

Inferred from electronic annotation. Source: Compara

   Cellular_componentlysosome

Inferred from direct assay PubMed 8702598. Source: RGD

   Molecular_functionbeta-mannosidase activity

Inferred from direct assay PubMed 8702598. Source: RGD

mannose binding

Inferred from direct assay PubMed 8702598. Source: RGD

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 Ref.1 (identifier: Q4FZV0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 Ref.1 (identifier: Q4FZV0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-383: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 881863Beta-mannosidase
PRO_0000250614

Sites

Active site4571Proton donor By similarity UniProtKB Q75W54

Amino acid modifications

Glycosylation351N-linked (GlcNAc...) Potential
Glycosylation771N-linked (GlcNAc...) Potential
Glycosylation891N-linked (GlcNAc...) Potential
Glycosylation1131N-linked (GlcNAc...) Potential
Glycosylation2261N-linked (GlcNAc...) Potential
Glycosylation2971N-linked (GlcNAc...) Potential
Glycosylation3021N-linked (GlcNAc...) Potential
Glycosylation8031N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 383383Missing in isoform 2. Ref.1
VSP_052170

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified August 30, 2005. Version 1.
Checksum: 023E3BE808CE6306

FASTA881101,221
        10         20         30         40         50         60 
MHLHLLFLLA LCGAGCMAAG PSYSLRGSWR VSNGNSSLEL PATVPGYVHS ALQQHGLIQD 

        70         80         90        100        110        120 
PYYRFNDLNY RWISLDNWTY STEFKIPFNR SEWQKVKLIF DGVDTVAEIL FNNVTIGKTD 

       130        140        150        160        170        180 
NMFTRYSFDV TNVVKDVNSL KLRFQSAVQY AECQSKAHTQ YRVPPECPPV EQKGECHVNF 

       190        200        210        220        230        240 
IRKEQCSFSW DWGPSFPSQG IWKDVRIEAY NIAHLDHLTF LPLYDNTSQA WTIEIEASFD 

       250        260        270        280        290        300 
VVSTKPVGGQ VTIAIPELKT QQANHIELQH GQRIVKLLVK IRKDVTVETW WPHGHGNQTG 

       310        320        330        340        350        360 
YNTTILFALD GGLKIEKAAK VYFRTVQLIE EPITGSPGLS FYFKINGLPI FLKGSNWIPA 

       370        380        390        400        410        420 
DSFQDKVTSE LLQLLLQSAV DANMNTLRVW GGGIYEQDEF YALCDELGIM VWQDFMFASA 

       430        440        450        460        470        480 
LYPTEPGFLE SVRKEVTYQV RRLKSHPSVI IWSGNNENEV ALRVNWFHVN PRDLGTYIND 

       490        500        510        520        530        540 
YVTLYVKTIR EIVLSEDRSR PFIASSPTNG VKTMTEGWIS KDPYSTQYGD MHFYDYFSDC 

       550        560        570        580        590        600 
WDWKVFPKAR LVSEYGYQSW PSFSTLQKVS RQEDWSYSSR FSLHRQHHGN GNNEMLHQVQ 

       610        620        630        640        650        660 
LHFQLPQRRD PVRAFKDTIY LTQVMQAQCI KTETEFYLRS RSEIVNGEGH TMGALYWQLN 

       670        680        690        700        710        720 
DIWQAPSWAS LEYGGKWKML HYFAQRFFAP LLPVGFEDEG VFYVYGVSDL HKDYPTKLTV 

       730        740        750        760        770        780 
RLHRWSSQKP LCTFVSLSAV IKAGEAMVLF QMPVSKLLKR CKECTRDTCV VSFYLSTDNE 

       790        800        810        820        830        840 
LFSPTNYHFL SSLKDAKGMV KANITVSISQ KGDLFVFDLK SSTSAITPFV WLDVGSIPGR 

       850        860        870        880 
FSDNGFLMIK KELSVLFYPW KPTSKSELQQ AFSVTSLTDL Y

« Hide

Isoform 2 [UniParc].

Checksum: E03D5EE26E6D60CF
Show »

FASTA49857,806

References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Placenta and Thymus.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BC099094 mRNA. Translation: AAH99094.1.
BC111710 mRNA. Translation: AAI11711.1.
IPIIPI00388355.
IPI00786836.
RefSeqNP_001026825.1. NM_001031655.1.
UniGeneRn.20578.

3D structure databases

ProteinModelPortalQ4FZV0.
ModBaseSearch...

Protein family/group databases

CAZyGH2. Glycoside Hydrolase Family 2.

Proteomic databases

PaxDbQ4FZV0.
PRIDEQ4FZV0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000073629; ENSRNOP00000064512; ENSRNOG00000013476.
GeneID310864.
KEGGrno:310864.

Organism-specific databases

CTD4126.
RGD1305785. Manba.

Phylogenomic databases

eggNOGCOG3250.
GeneTreeENSGT00390000001670.
HOGENOMHOG000186862.
HOVERGENHBG052404.
InParanoidQ4FZV0.
KOK01192.
OMANRYSFDI.
OrthoDBEOG40VVP2.

Enzyme and pathway databases

SABIO-RKQ4FZV0.
UniPathwayUPA00280.

Gene expression databases

GenevestigatorQ4FZV0.
GermOnlineENSRNOG00000013476. Rattus norvegicus.

Family and domain databases

Gene3D3.20.20.80. 2 hits.
InterProIPR008979. Galactose-bd-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
SUPFAMSSF49785. Gal_bind_like. 1 hit.
SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNetSearch...

Other

BindingDBQ4FZV0.
ChEMBLCHEMBL3431.
NextBio662767.

Entry information

Entry nameMANBA_RAT
AccessionPrimary (citable) accession number: Q4FZV0
Secondary accession number(s): Q2NKP5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: August 30, 2005
Last modified: May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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