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Protein

Protein phosphatase methylesterase 1

Gene

Ppme1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Demethylates proteins that have been reversibly carboxymethylated. Demethylates PPP2CB (in vitro) and PPP2CA. Binding to PPP2CA displaces the manganese ion and inactivates the enzyme (By similarity).By similarity1 Publication

Catalytic activityi

[Phosphatase 2A protein]-leucine methyl ester + H2O = [phosphatase 2A protein]-leucine + methanol.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei156 – 1561By similarity
Active sitei349 – 3491By similarity

GO - Molecular functioni

  • protein C-terminal methylesterase activity Source: Ensembl
  • protein kinase binding Source: UniProtKB
  • protein phosphatase type 2A regulator activity Source: UniProtKB

GO - Biological processi

  • protein demethylation Source: Ensembl
  • regulation of sodium ion transport Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase methylesterase 1 (EC:3.1.1.89)
Short name:
PME-1
Gene namesi
Name:Ppme1
Synonyms:Pme1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1309683. Ppme1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 386385Protein phosphatase methylesterase 1PRO_0000413635Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei15 – 151PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated by SIK1 following increases in intracellular sodium, leading to dissociation from the protein phosphatase 2A (PP2A) complex and subsequent dephosphorylation of sodium/potassium-transporting ATPase ATP1A1.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ4FZT2.
PRIDEiQ4FZT2.

PTM databases

iPTMnetiQ4FZT2.
PhosphoSiteiQ4FZT2.

Expressioni

Gene expression databases

ExpressionAtlasiQ4FZT2. baseline and differential.
GenevisibleiQ4FZT2. RN.

Interactioni

Subunit structurei

Binds PPP2CA and PPP2CB.By similarity

GO - Molecular functioni

  • protein kinase binding Source: UniProtKB

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023649.

Structurei

3D structure databases

ProteinModelPortaliQ4FZT2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi256 – 2638Poly-Glu

Sequence similaritiesi

Belongs to the AB hydrolase superfamily.Curated

Phylogenomic databases

eggNOGiKOG2564. Eukaryota.
COG0596. LUCA.
HOGENOMiHOG000212435.
InParanoidiQ4FZT2.
KOiK13617.
OMAiRPWRWRT.
OrthoDBiEOG091G0D0P.
TreeFamiTF314697.

Family and domain databases

Gene3Di3.40.50.1820. 2 hits.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR016812. PPase_methylesterase_euk.
[Graphical view]
PANTHERiPTHR14189. PTHR14189. 1 hit.
PfamiPF12697. Abhydrolase_6. 1 hit.
[Graphical view]
PIRSFiPIRSF022950. PPase_methylesterase_euk. 1 hit.
SUPFAMiSSF53474. SSF53474. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4FZT2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSALEKSMHL GRLPSRPPLP GSGGSQSGAK MRMGPGRKRD FTPVPWSQYF
60 70 80 90 100
ESMEDVEVEN ETGKDTFRVY KSGSEGPVLL LLHGGGHSAL SWAVFTAAII
110 120 130 140 150
SRVQCRIVAL DLRSHGETKV KNSEDLSAET MAKDVGNVVE AMYGDLPPPV
160 170 180 190 200
MLIGHSMGGA IAVHTAAANL VPSLLGLCMI DVVEGTAMDA LNSMQNFLRG
210 220 230 240 250
RPKTFKSLEN AIEWSVKSGQ IRNLESARVS MVGQVKQCEG ITSPESSKSI
260 270 280 290 300
VEGIIEEEEE DEEGSESVNK RKKEDDMETK KDHPYTWRIE LAKTEKYWDG
310 320 330 340 350
WFRGLSNLFL SCPIPKLLLL AGVDRLDKDL TIGQMQGKFQ MQVLPQCGHA
360 370 380
VHEDAPDKVA EAVATFLIRH RFAEPIGGFQ CVFPGC
Length:386
Mass (Da):42,316
Last modified:October 19, 2011 - v2
Checksum:i2D176042469C9E29
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC099160 mRNA. Translation: AAH99160.1.
RefSeqiNP_001178767.1. NM_001191838.1.
UniGeneiRn.137659.

Genome annotation databases

EnsembliENSRNOT00000023648; ENSRNOP00000023649; ENSRNOG00000017227.
GeneIDi361613.
KEGGirno:361613.
UCSCiRGD:1309683. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC099160 mRNA. Translation: AAH99160.1.
RefSeqiNP_001178767.1. NM_001191838.1.
UniGeneiRn.137659.

3D structure databases

ProteinModelPortaliQ4FZT2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023649.

PTM databases

iPTMnetiQ4FZT2.
PhosphoSiteiQ4FZT2.

Proteomic databases

PaxDbiQ4FZT2.
PRIDEiQ4FZT2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000023648; ENSRNOP00000023649; ENSRNOG00000017227.
GeneIDi361613.
KEGGirno:361613.
UCSCiRGD:1309683. rat.

Organism-specific databases

CTDi51400.
RGDi1309683. Ppme1.

Phylogenomic databases

eggNOGiKOG2564. Eukaryota.
COG0596. LUCA.
HOGENOMiHOG000212435.
InParanoidiQ4FZT2.
KOiK13617.
OMAiRPWRWRT.
OrthoDBiEOG091G0D0P.
TreeFamiTF314697.

Miscellaneous databases

PROiQ4FZT2.

Gene expression databases

ExpressionAtlasiQ4FZT2. baseline and differential.
GenevisibleiQ4FZT2. RN.

Family and domain databases

Gene3Di3.40.50.1820. 2 hits.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR016812. PPase_methylesterase_euk.
[Graphical view]
PANTHERiPTHR14189. PTHR14189. 1 hit.
PfamiPF12697. Abhydrolase_6. 1 hit.
[Graphical view]
PIRSFiPIRSF022950. PPase_methylesterase_euk. 1 hit.
SUPFAMiSSF53474. SSF53474. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiPPME1_RAT
AccessioniPrimary (citable) accession number: Q4FZT2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: October 19, 2011
Last modified: September 7, 2016
This is version 58 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.