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Protein

Nesprin-3

Gene

Syne3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system. Plays a role in the regulation of aortic epithelial cell morphology, and is required for flow-induced centrosome polarization and directional migration in aortic endothelial cells (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
Nesprin-3
Alternative name(s):
KASH domain-containing protein 3
Short name:
KASH3
Nuclear envelope spectrin repeat protein 3
Gene namesi
Name:Syne3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:2442408. Syne3.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 925CytoplasmicPROSITE-ProRule annotationAdd BLAST925
Transmembranei926 – 946Helical; Anchor for type IV membrane proteinPROSITE-ProRule annotationAdd BLAST21
Topological domaini947 – 975Perinuclear spacePROSITE-ProRule annotationAdd BLAST29

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002811211 – 975Nesprin-3Add BLAST975

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi953Interchain (C-577 in SUN2); alternateBy similarity
Disulfide bondi953Interchain (with C-759 in SUN1)By similarity

Post-translational modificationi

The disulfid bond with SUN1 or SUN2 is required for stability of the respective LINC complex under tensile forces.By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiQ4FZC9.
PaxDbiQ4FZC9.
PeptideAtlasiQ4FZC9.
PRIDEiQ4FZC9.

PTM databases

iPTMnetiQ4FZC9.
PhosphoSitePlusiQ4FZC9.
SwissPalmiQ4FZC9.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiENSMUSG00000054150.
CleanExiMM_4831426I19RIK.
GenevisibleiQ4FZC9. MM.

Interactioni

Subunit structurei

Core component of LINC complexes which are composed of inner nuclear membrane SUN domain-containing proteins coupled to outer nuclear membrane KASH domain-containing nesprins. SUN and KASH domain-containing proteins seem to bind each other promiscuously; however, differentially expression of LINC complex constituents can give rise to specific assemblies. Interacts with SUN1 and SUN2; probably forming respective LINC complexes. Interacts with PLEC (via actin-binding domain). Interacts with DST. Interacts with SYNE1. Interacts (via KASH domain) with TOR1A (ATP-bound); the interaction is required for SYNE3 nuclear envelope localization.4 Publications

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ4FZC9. 2 interactors.
STRINGi10090.ENSMUSP00000093090.

Structurei

3D structure databases

ProteinModelPortaliQ4FZC9.
SMRiQ4FZC9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati220 – 325Spectrin 1Add BLAST106
Repeati647 – 740Spectrin 2Add BLAST94
Domaini917 – 975KASHPROSITE-ProRule annotationAdd BLAST59

Domaini

The KASH domain is involved in the binding to SUN1 and SUN2 through recognition of their SUN domains.By similarity

Sequence similaritiesi

Belongs to the nesprin family.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410KD4G. Eukaryota.
ENOG4111CQ9. LUCA.
GeneTreeiENSGT00440000039367.
HOGENOMiHOG000154475.
HOVERGENiHBG094014.
InParanoidiQ4FZC9.
KOiK21761.
OMAiIKAQWEE.
OrthoDBiEOG091G01LN.
PhylomeDBiQ4FZC9.
TreeFamiTF331132.

Family and domain databases

InterProiView protein in InterPro
IPR012315. KASH.
IPR018159. Spectrin/alpha-actinin.
IPR002017. Spectrin_repeat.
IPR030267. SYNE3.
PANTHERiPTHR44810. PTHR44810. 1 hit.
PfamiView protein in Pfam
PF10541. KASH. 1 hit.
PF00435. Spectrin. 2 hits.
SMARTiView protein in SMART
SM01249. KASH. 1 hit.
SM00150. SPEC. 3 hits.
PROSITEiView protein in PROSITE
PS51049. KASH. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q4FZC9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTQQPQEDFE RSVEDAQAWM KVIQEQLQVN DNTKGPRAAL EARLRETEKI
60 70 80 90 100
CQLESEGMVK VELVLRAAEA LLATCQEGQK PEILARLRDI KSQWEETVTY
110 120 130 140 150
MTHCHSRIEW VWLHWSEYLL AQDEFYRWFQ KMVVALEPPV ELQLGLKEKQ
160 170 180 190 200
WQLSHAQVLL HNVDNQAVLL DRLLEEAGSL FSRIGDPSVD EDAQKRMKAE
210 220 230 240 250
YDAVKARAQR RVDLLAQVAQ DHEQYREDVN EFQLWLKAVV EKVHSCLGRN
260 270 280 290 300
CKLATELRLS TLQDIAKDFP RGEESLKRLE EQAVGVIQNT SPLGAEKISG
310 320 330 340 350
ELEEMRGVLE KLRVLWKEEE GRLRGLLQSR GDCEQQIQQL EAELGDFKKS
360 370 380 390 400
LQRLAQEGLE PTVKTATEDE LVAQWRLFSG TRAALASEEP RVDRLQTQLK
410 420 430 440 450
KLVTFPDLQS LSDSVVATIQ EYQSMKGKNT RLHNATRAEL WQRFQRPLND
460 470 480 490 500
LQLWKALAQR LLDITASLPD LASIHTFLPQ IEAALTESSR LKEQLAMLQL
510 520 530 540 550
KTDLLGSIFG QERAATLLEQ VTSSVRDRDL LHNSLLQRKS KLQSLLVQHK
560 570 580 590 600
DFGVAFDPLN RKLLDLQARI QAEKGLPRDL PGKQVQLLRL QGLQEEGLDL
610 620 630 640 650
GTQIEAVRPL AHGNSKHQQK VDQISCDQQA LQRSLEDLVD RCQQNVREHC
660 670 680 690 700
TFSHRLSELQ LWITMATQTL ESHQGDVRLW DAESQEAGLE TLLSEIPEKE
710 720 730 740 750
VQVSLLQALG QLVMKKSSPE GATMVQEELR KLMESWQALR LLEENMLSLM
760 770 780 790 800
RNQQLQRTEV DTGKKQVFTN NIPKAGFLIN PQDPIPRRQH GANPLEGHDL
810 820 830 840 850
PEDHPQLLRD FEQWLQAENS KLRRIITMRV ATAKDLRTRE VKLQELEARI
860 870 880 890 900
PEGQHLFENL LRLRPARDPS NELEDLRYRW MLYKSKLKDS GHLLTESSPG
910 920 930 940 950
ELTAFQKSRR QKRWSPCSLL QKACRVALPL QLLLLLFLLL LFLLPAGEEE
960 970
RSCALANNFA RSFALMLRYN GPPPT
Length:975
Mass (Da):112,035
Last modified:August 30, 2005 - v1
Checksum:i26D80A295CE0CF8B
GO
Isoform 2 (identifier: Q4FZC9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-87: Missing.
     88-105: RDIKSQWEETVTYMTHCH → MTTRRGPERPWRQGFERQ

Note: No experimental confirmation available.
Show »
Length:888
Mass (Da):102,197
Checksum:iB7A7F195E6A61587
GO

Sequence cautioni

The sequence BAC26351 differs from that shown. Reason: Frameshift at position 776.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0239791 – 87Missing in isoform 2. 1 PublicationAdd BLAST87
Alternative sequenceiVSP_02398088 – 105RDIKS…MTHCH → MTTRRGPERPWRQGFERQ in isoform 2. 1 PublicationAdd BLAST18

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK029216 mRNA. Translation: BAC26351.1. Frameshift.
AK030542 mRNA. Translation: BAC27012.1.
BC099694 mRNA. Translation: AAH99694.1.
CCDSiCCDS36542.1. [Q4FZC9-1]
CCDS36543.1. [Q4FZC9-2]
RefSeqiNP_001036164.1. NM_001042699.2. [Q4FZC9-1]
NP_766088.2. NM_172500.3. [Q4FZC9-2]
XP_006515715.2. XM_006515652.3. [Q4FZC9-1]
XP_006515716.1. XM_006515653.2. [Q4FZC9-1]
XP_006515717.1. XM_006515654.3. [Q4FZC9-1]
UniGeneiMm.212688.

Genome annotation databases

EnsembliENSMUST00000067005; ENSMUSP00000065771; ENSMUSG00000054150. [Q4FZC9-2]
ENSMUST00000095439; ENSMUSP00000093090; ENSMUSG00000054150. [Q4FZC9-1]
ENSMUST00000109927; ENSMUSP00000105553; ENSMUSG00000054150. [Q4FZC9-2]
GeneIDi212073.
KEGGimmu:212073.
UCSCiuc007oxr.2. mouse. [Q4FZC9-1]
uc007oxs.2. mouse. [Q4FZC9-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSYNE3_MOUSE
AccessioniPrimary (citable) accession number: Q4FZC9
Secondary accession number(s): Q8BMM1, Q8C117
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: August 30, 2005
Last modified: October 25, 2017
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families