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Q4FUP5

- GLND_PSYA2

UniProt

Q4FUP5 - GLND_PSYA2

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Psychrobacter arcticus (strain DSM 17307 / 273-4)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciPARC259536:GI3A-410-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:Psyc_0395
OrganismiPsychrobacter arcticus (strain DSM 17307 / 273-4)
Taxonomic identifieri259536 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter
ProteomesiUP000000546: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 915915Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_0000231688Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi259536.Psyc_0395.

Structurei

3D structure databases

ProteinModelPortaliQ4FUP5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini498 – 58891HDUniRule annotationAdd
BLAST
Domaini732 – 81786ACT 1UniRule annotationAdd
BLAST
Domaini840 – 91576ACT 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 360360UridylyltransferaseAdd
BLAST
Regioni361 – 731371Uridylyl-removingAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains 1 HD domain.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4FUP5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFNCAVTAID LTPMPLLSTD NMTTAPLSLS TDTSLIEKSL FGIPEWLLQI
60 70 80 90 100
NDDISRALER GVNIRQLVSA RACVIDDLLI GLFKWFELDK TDLALFATGG
110 120 130 140 150
YGRGELSLHS DIDILLLMPH EIDADTSSKI DNLVALLWDI GLEPALSVRS
160 170 180 190 200
VSECLEAALD HTIASALLEA RLLIGNETLQ DVPHQIVNNQ WSPRAFYDVK
210 220 230 240 250
IDEAKARYLQ HNATEYNLEP NIKTAPGGLR DIHIVGWVTK RYFRVSKLYD
260 270 280 290 300
LVQQNFLTEK EFDELSFSEG YLWQIRHYLH ELTGRNENKL LFDYQREIAQ
310 320 330 340 350
LMGYDTQPDD QPNAAVERFM RDYYRCAMQI STLSEMLTNH YYETIIEPQL
360 370 380 390 400
PDEERPKKQP INARFNQVGE QIAMAHHRVF AQHPESILEM FLLMGQYGIK
410 420 430 440 450
NVRTHTLRAL KIAARGIDQA YRDNPTHQTL FLANLKEQNY LFHRLRTMNR
460 470 480 490 500
YGVLGNYIPA FAQVTGLMQY DLFHRYTVDA HTLFLIRILH RFIDPHFYED
510 520 530 540 550
FPLVSSIFQR IERKEILVLA AMFHDIAKGR GGNHSQLGEI ESIEFCLAHG
560 570 580 590 600
MSNADANLVG WLTRYHLLMS MTAQKKDISD PEVVTLFADL VGNVTHLNHL
610 620 630 640 650
YVLTVADMNA TNPQLWNSWR ATLMKQLYSQ TRRILRADID APTNRQDMIS
660 670 680 690 700
ATRKQALMML DNVDNQHMNR DEVLSLWDDL GDEYFLREIA EDILWHTEAI
710 720 730 740 750
LNHPPIGRAS NADSPPLVVL REHRELALDA VQVFVYTQDQ VNLFAVTMAV
760 770 780 790 800
FDQMNLDVLD ARIITATRDF ALDSYVLLDR SGTLLVDSDS QQELKQRLID
810 820 830 840 850
AFKNPTAPKL THKRIPRQLK HFDVETTINF EFNDASSQHI MSLETLDQPG
860 870 880 890 900
LLARVGQVFL QQQIEVHAAR ITTLGERAED MFYISDQNDQ ALSANKLKTL
910
KTALIESLSV HNDSI
Length:915
Mass (Da):104,846
Last modified:August 30, 2005 - v1
Checksum:i68837CAE3D605366
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000082 Genomic DNA. Translation: AAZ18263.1.
RefSeqiYP_263697.1. NC_007204.1.

Genome annotation databases

EnsemblBacteriaiAAZ18263; AAZ18263; Psyc_0395.
GeneIDi3514133.
KEGGipar:Psyc_0395.
PATRICi23055115. VBIPsyArc98534_0490.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000082 Genomic DNA. Translation: AAZ18263.1 .
RefSeqi YP_263697.1. NC_007204.1.

3D structure databases

ProteinModelPortali Q4FUP5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 259536.Psyc_0395.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ18263 ; AAZ18263 ; Psyc_0395 .
GeneIDi 3514133.
KEGGi par:Psyc_0395.
PATRICi 23055115. VBIPsyArc98534_0490.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci PARC259536:GI3A-410-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Psychrobacter arcticus 273-4, a psychroactive Siberian permafrost bacterium, reveals mechanisms for adaptation to low-temperature growth."
    Ayala-del-Rio H.L., Chain P.S., Grzymski J.J., Ponder M.A., Ivanova N., Bergholz P.W., Di Bartolo G., Hauser L., Land M., Bakermans C., Rodrigues D., Klappenbach J., Zarka D., Larimer F., Richardson P., Murray A., Thomashow M., Tiedje J.M.
    Appl. Environ. Microbiol. 76:2304-2312(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 17307 / 273-4.

Entry informationi

Entry nameiGLND_PSYA2
AccessioniPrimary (citable) accession number: Q4FUP5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: August 30, 2005
Last modified: October 29, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3