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Q4FU71 (SYL_PSYA2) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Psyc_0576
OrganismPsychrobacter arcticus (strain DSM 17307 / 273-4) [Complete proteome] [HAMAP]
Taxonomic identifier259536 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter

Protein attributes

Sequence length921 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 921921Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334797

Regions

Motif80 – 9011"HIGH" region HAMAP-Rule MF_00049
Motif667 – 6715"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6701ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4FU71 [UniParc].

Last modified August 30, 2005. Version 1.
Checksum: A3E4AA9983B25748

FASTA921103,244
        10         20         30         40         50         60 
MLWHYKISPH LSVITNSEPT MSNAVTAETA NTNNTSIENA QYQPQLIEAA QQAKWATDKR 

        70         80         90        100        110        120 
FEVSNEPSDK PSRYMLSMFP YPSGKLHMGH VRNYTISDVL SRYYRLKGYE VMQPMGWDGF 

       130        140        150        160        170        180 
GLPAENAAIA NQTPPAKWTF ENIDSMRAQL KLLGLSIDWS REFATCSPEY YQWEQWLFLQ 

       190        200        210        220        230        240 
LYKKGLVYKK LATVNWDPID NTVLANEQVI DGKGWRSGAM VEKRDIPMYY FNITDYADEL 

       250        260        270        280        290        300 
LDDLDQLEGH WPSEVITMQR NWIGRSSGME VHFPYELAGE SNSLDVFTTR PDTLMGVTYV 

       310        320        330        340        350        360 
AVAAEHPLAQ YASEHNEAIA AFCALCKKGS VAEADLAKAE KVGIDTGLTV THPLTGEEVP 

       370        380        390        400        410        420 
VWVANYVLMS YGSGAVMAVP AHDERDYEFA TKYSLPIKQV IDIPARYFDD IEEGNDNRAY 

       430        440        450        460        470        480 
TERNTLVNSG EFDGMDFEQA FAAMLAKLEP QSLAKKKIQY RLRDWGVSRQ RYWGCPIPMV 

       490        500        510        520        530        540 
NCEHCGTVPV EEQDLPVILP TDVVPDGRGN PLKNIPEFVN TTCPKCGNPA ERETDTFDTF 

       550        560        570        580        590        600 
VESSWYYARF ASPNDTTNMV NKSAANKWLP VDQYIGGVEH AVMHLLYARF FHKLMRDENL 

       610        620        630        640        650        660 
VSGDEPFANL MTQGMVLAGT FYRVNPDGST TYYFTKDIDI DFNERGQPIK AILKSDGQPV 

       670        680        690        700        710        720 
TIGKIEKMSK SKNNGVDPQI TIDKYGADTV RLYTLFTAPA DQTLEWSDDA LKGPYNFVKK 

       730        740        750        760        770        780 
VWRIASEHMQ ALTAANLSLD TLNNGTLNND ALNTEGLSKE AKALRRKTHE TIGKIDSDLG 

       790        800        810        820        830        840 
KRLALNTPVS SLMELANELS NFKANSEQEL QVQHEALIDL LIMLSVYAPH IGEHLLEQLG 

       850        860        870        880        890        900 
LDTVTLNYPA VDESALVQDM ITMVIQVNGK MRGKMDVAPN SDPEALKAQA RAIEGVAKFL 

       910        920 
TGEIKKEIVV PNKLVNIVVA G 

« Hide

References

[1]"The genome sequence of Psychrobacter arcticus 273-4, a psychroactive Siberian permafrost bacterium, reveals mechanisms for adaptation to low-temperature growth."
Ayala-del-Rio H.L., Chain P.S., Grzymski J.J., Ponder M.A., Ivanova N., Bergholz P.W., Di Bartolo G., Hauser L., Land M., Bakermans C., Rodrigues D., Klappenbach J., Zarka D., Larimer F., Richardson P., Murray A., Thomashow M., Tiedje J.M.
Appl. Environ. Microbiol. 76:2304-2312(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 17307 / 273-4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000082 Genomic DNA. Translation: AAZ18437.1.
RefSeqYP_263871.1. NC_007204.1.

3D structure databases

ProteinModelPortalQ4FU71.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING259536.Psyc_0576.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ18437; AAZ18437; Psyc_0576.
GeneID3514464.
KEGGpar:Psyc_0576.
PATRIC23055543. VBIPsyArc98534_0695.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycPARC259536:GI3A-600-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_PSYA2
AccessionPrimary (citable) accession number: Q4FU71
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: August 30, 2005
Last modified: April 16, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries