Reviewed, UniProtKB/Swiss-Prot Q4FSU1 (CYSG_PSYA2)

Names and origin

Protein names

Recommended name:
    Siroheme synthase
Including the following 3 domains:
    1- Recommended name:
            Uroporphyrinogen-III C-methyltransferase
                Short name=Urogen III methylase
              EC=2.1.1.107
        Alternative name(s):
            SUMT
            Uroporphyrinogen III methylase
              Short name=UROM
    2- Recommended name:
            Precorrin-2 dehydrogenase
              EC=1.3.1.76
    3- Recommended name:
            Sirohydrochlorin ferrochelatase
              EC=4.99.1.4

Gene names

Name:	cysG
Ordered Locus Names:	Psyc_1065

Organism

Psychrobacter arcticus (strain DSM 17307 / 273-4) [Complete proteome] [HAMAP]

Taxonomic identifier

259536 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Psychrobacter

Protein attributes

Sequence length	549 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into siroheme. This reaction consists of the NAD-dependent oxidation of precorrin-2 into sirohydrochlorin and its subsequent ferrochelation into siroheme By similarity. HAMAP MF_01646
Catalytic activity	S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1. HAMAP MF_01646 S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2. HAMAP MF_01646 Precorrin-2 + NAD+ = sirohydrochlorin + NADH. HAMAP MF_01646 Siroheme + 2 H+ = sirohydrochlorin + Fe2+. HAMAP MF_01646
Pathway	Cofactor biosynthesis; adenosylcobalamin biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1. HAMAP MF_01646 Cofactor biosynthesis; adenosylcobalamin biosynthesis; sirohydrochlorin from precorrin-2: step 1/1. Porphyrin metabolism; siroheme biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1. HAMAP MF_01646 Porphyrin metabolism; siroheme biosynthesis; siroheme from sirohydrochlorin: step 1/1. HAMAP MF_01646 Porphyrin metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.
Sequence similarities	Belongs to the precorrin methyltransferase family.

Ontologies

Keywords
Biological process	Cobalamin biosynthesis Porphyrin biosynthesis
Ligand	NAD S-adenosyl-L-methionine
Molecular function	Lyase Methyltransferase Oxidoreductase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	cobalamin biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW siroheme biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	binding Inferred from electronic annotation. Source: InterPro precorrin-2 dehydrogenase activity Inferred from electronic annotation. Source: HAMAP sirohydrochlorin ferrochelatase activity Inferred from electronic annotation. Source: EC uroporphyrin-III C-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 549	549	Siroheme synthase HAMAP MF_01646		PRO_0000330546
Regions
	Region	249 – 491	243	Uroporphyrinogen-III C-methyltransferase HAMAP MF_01646

Sequences

	Sequence		Length	Mass (Da)
	Q4FSU1-1 [UniParc]. Last modified April 29, 2008. Version 2. Checksum: 019AACCDA20937C4	FASTA	549	59,727
	10 20 30 40 50 60 MNTFPLFFKL EDRKVLIVGG GDVALRKADL LSRAGACITV LAPSINHEIQ ALLSSSKHEL 70 80 90 100 110 120 IYEHYNKTYM TNSRVIIAAT DDERLNHQIH ADATALNIPV NVVDTPHLCD FIFPAIVDRN 130 140 150 160 170 180 PIVIGISSNG KAPVLARLLR ARLETLIPQG YGKLAKLAGE FRGDVKAKIP TLTGRRQFWE 190 200 210 220 230 240 RAFEGKVSQL MFAGNENEAL AQLQADLDNT AASITAKDAT DDNGLSTAST SAPAIANEFT 250 260 270 280 290 300 AARNTMGEVY IVGAGPGDPE LLTFKALRLM QQADIVYYDA LVSPQVLDLC RRDADKVFVG 310 320 330 340 350 360 KKRRNHAVAQ LGINELLVNS AKEGRRVVRL KGGDPFIFGR GGEEIESLRA HNIPYQVVPG 370 380 390 400 410 420 ITAANAAASY AGIPLTHRDH SQSVRFVTGF LKAGAPNNNF KNFLDTDETV VFYMGLHSLP 430 440 450 460 470 480 RLTEGLIDAG RSAETPIAIV SNASMPNQQV LTGTLASIVE LQEKNQLPTP ALLIMGDVVS 490 500 510 520 530 540 LHHDLAWYNL HNQQHNQNTS ETDNNWLRGG TATTPKSNAQ FNAHQQAHAL SMITNLATED GDLEQLIIG

References

[1]

"Complete sequence of Psychrobacter arcticum 273-4." NASA Astrobiology Institute Ayala-del-Rio H.L., Chain P., Ponder M.A., Di Bartolo G., Ivanova N., Bergholz P.W., Hauser L., Land M., Bakermans C., Rodrigues D., Klappenbach J.A., Zarka D., Larimer F., Richardson P., Thomashow M.F., Tiedje J.M. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000082 Genomic DNA. Translation: AAZ18917.1. Different initiation.
RefSeq	YP_264351.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q4FSU1.
Genome annotation databases
GeneID	3514951.
GenomeReviews	Gene locus Psyc_1065 in contig CP000082_GR.
KEGG	par:Psyc_1065.
NMPDR	fig|259536.4.peg.646.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0007.
HOGENOM	HBG730212.
OMA	THRDVAD.
PhylomeDB	Q4FSU1.
Enzyme and pathway databases
BioCyc	PARC259536:PSYC_1065-MONOMER.
Family and domain databases
HAMAP	MF_01646. Siroheme_synth. [Tree]
InterPro	IPR000878. 4pyrrol_Mease. IPR014777. 4pyrrole_Mease_sub1. IPR014776. 4pyrrole_Mease_sub2. IPR006366. CobA_cysG_C. IPR016040. NAD(P)-bd_dom. IPR019478. Sirohaem_synthase_dimer_dom. IPR006367. Sirohaem_synthase_N. IPR003043. Uropor_MeTrfase_CS. [Graphical view]
Gene3D	G3DSA:3.40.1010.10. 4pyrrole_Mease_sub1. 1 hit. G3DSA:3.30.950.10. 4pyrrole_Mease_sub2. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF10414. CysG_dimeriser. 1 hit. PF00590. TP_methylase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01469. cobA_cysG_Cterm. 1 hit. TIGR01470. cysG_Nterm. 1 hit.
PROSITE	PS00839. SUMT_1. False negative. PS00840. SUMT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSG_PSYA2

Accession

Primary (citable) accession number: Q4FSU1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	April 29, 2008
Last modified:	February 9, 2010
This is version 34 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)