NAD-dependent malic enzyme - Psychrobacter arcticus (strain DSM 17307 / 273-4)

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Reviewed, UniProtKB/Swiss-Prot Q4FRX3 (MAO1_PSYA2)

Last modified November 25, 2008. Version 26. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
    NAD-dependent malic enzyme
      Short name=NAD-ME
    EC=1.1.1.38

Gene names

Name:	sfcA
Ordered Locus Names:	Psyc_1387

Organism

Psychrobacter arcticus (strain DSM 17307 / 273-4) [Complete proteome] [HAMAP]

Taxonomic identifier

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Psychrobacter

Protein attributes

Sequence length	560 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD(+) = pyruvate + CO(2) + NADH. HAMAP MF_01619
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

560

NAD-dependent malic enzyme HAMAP MF_01619

PRO_0000160227

Sites

Active site

1

Proton donor By similarity

Active site

1

Proton acceptor By similarity

Metal binding

1

Divalent metal cation By similarity

Metal binding

1

Divalent metal cation By similarity

Metal binding

1

Divalent metal cation By similarity

Binding site

1

NAD By similarity

Binding site

1

NAD By similarity

Binding site

1

NAD By similarity

Site

1

Important for activity By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q4FRX3-1 [UniParc].

Last modified August 30, 2005. Version 1.
Checksum: 2AF5AE968858C1D2
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560

62,676

        10         20         30         40         50         60 
MPNQRPLYIP FSGPALLETP LLNKGSAFSS EERDSFNLTG LLPHNIETIE EQSLRAYHQL 

        70         80         90        100        110        120 
RSFTTDIDKH IYLRNIQDTN ETLFHHLIEQ HIEEVMPLIY TPTVGQACEK FSQIYRRKRG 

       130        140        150        160        170        180 
LFISYPDRHK IDDMLQNATK QKVKVIVVTD GERILGLGDQ GIGGMGIPIG KLALYTACGG 

       190        200        210        220        230        240 
ISPAYCLPIL LDVGTNNQQL LDDPMYMGWR NQRISGDEYN EFVDLFIQAV KRRWPEVLLQ 

       250        260        270        280        290        300 
FEDFAQENAT PLLNRYRDQL CCFNDDIQGT AAVSVGTLIA ACLNKGQKLS QQKIAFLGAG 

       310        320        330        340        350        360 
SAGCGIAEHI IRQMQREGLT EEQARKQVFM VDRYGLLTDS MTELQKFQAP LVQKESAIES 

       370        380        390        400        410        420 
WDKSQKLGLA QVVKQAKITV LFGVSGQKGL FTREVIEALC VNTEHPIVLP LSNPTSRVEA 

       430        440        450        460        470        480 
TPQEVTSWSR GKAIVATGSP FPNTTFEDQS FEISQCNNSY IFPGIGLGVL AAHATGISDN 

       490        500        510        520        530        540 
MLMSASQALA DISMEYEKAP GAILPPIKFI REISEKIAYA VALQAIEDKL ALPVTAENLE 

       550        560 
RRLKANFWLP RYHNYRRTSF

References

[1]

"Complete sequence of Psychrobacter arcticum 273-4."
NASA Astrobiology Institute
Ayala-del-Rio H.L., Chain P., Ponder M.A., Di Bartolo G., Ivanova N., Bergholz P.W., Hauser L., Land M., Bakermans C., Rodrigues D., Klappenbach J.A., Zarka D., Larimer F., Richardson P., Thomashow M.F., Tiedje J.M.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
	CP000082 Genomic DNA. Translation: AAZ19235.1.
RefSeq	YP_264669.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3515345.
GenomeReviews	Gene locus Psyc_1387 in contig CP000082_GR.
KEGG	par:Psyc_1387.
NMPDR	fig\|259536.4.peg.1046.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4FRX3.
Enzyme and pathway databases
BioCyc	PARC259536:PSYC_1387-MON.
Family and domain databases
HAMAP	MF_01619. [Tree]
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAO1_PSYA2

Accession

Primary (citable) accession number: Q4FRX3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	August 30, 2005
Last modified:	November 25, 2008
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents