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Q4FRI6

- TGT_PSYA2

UniProt

Q4FRI6 - TGT_PSYA2

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Protein
Queuine tRNA-ribosyltransferase
Gene
tgt, Psyc_1524
Organism
Psychrobacter arcticus (strain DSM 17307 / 273-4)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity.UniRule annotation

Catalytic activityi

Guanine(34) in tRNA + queuine = queuosine(34) in tRNA + guanine.UniRule annotation
Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine.UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei92 – 921Nucleophile By similarity
Binding sitei93 – 931Substrate By similarity
Metal bindingi310 – 3101Zinc By similarity
Metal bindingi312 – 3121Zinc By similarity
Metal bindingi315 – 3151Zinc By similarity
Metal bindingi341 – 3411Zinc By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. queuine tRNA-ribosyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. queuosine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Queuosine biosynthesis, tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciPARC259536:GI3A-1560-MONOMER.
UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
Queuine tRNA-ribosyltransferase (EC:2.4.2.29)
Alternative name(s):
Guanine insertion enzyme
tRNA-guanine transglycosylase
Gene namesi
Name:tgt
Ordered Locus Names:Psyc_1524
OrganismiPsychrobacter arcticus (strain DSM 17307 / 273-4)
Taxonomic identifieri259536 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter
ProteomesiUP000000546: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 383383Queuine tRNA-ribosyltransferaseUniRule annotation
PRO_1000203659Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi259536.Psyc_1524.

Structurei

3D structure databases

ProteinModelPortaliQ4FRI6.
SMRiQ4FRI6. Positions 13-372.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0343.
HOGENOMiHOG000223473.
KOiK00773.
OMAiKYNSRSH.
OrthoDBiEOG6SNDVG.

Family and domain databases

Gene3Di3.20.20.105. 1 hit.
HAMAPiMF_00168. Q_tRNA_Tgt.
InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PANTHERiPTHR11962. PTHR11962. 1 hit.
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.

Sequencei

Sequence statusi: Complete.

Q4FRI6-1 [UniParc]FASTAAdd to Basket

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MQFVLHKTAS GVTRARRGTV HLNHGDVQTP AFMPVGTYGT VKGMLPRDIE    50
AIGADIILGN TFHLWLRPGT DIIDKFGGLH KFMHWDKPIL TDSGGFQVFS 100
LGAMRKITEE GVTFKSPIDG AKVFLSPEKS MQIQYSLNSD IVMQFDECTP 150
YPATHDEAKK SLELSLRWGQ RCVDEHKKLG STNALFGIIQ GSMYADLRKQ 200
SLEGLLEIGF DGYAIGGLSV GEPKEEMIDV LDYIANDMPA DKPRYLMGVG 250
KPEDLVEGVR RGVDMFDCVM PTRNARNGHY FVTGDVDNAG VVRIRNSQYR 300
TDEGPLDPEC DCYTCQNFSR AYLSHLNKCK EMLGAQLATI HNLRYYQRLM 350
QGIRDAIEQD KFDEFVTEFY SKRGQTVPEL NLR 383
Length:383
Mass (Da):43,068
Last modified:August 30, 2005 - v1
Checksum:iD45D7218A5FD5FD0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000082 Genomic DNA. Translation: AAZ19372.1.
RefSeqiYP_264806.1. NC_007204.1.

Genome annotation databases

EnsemblBacteriaiAAZ19372; AAZ19372; Psyc_1524.
GeneIDi3515485.
KEGGipar:Psyc_1524.
PATRICi23057765. VBIPsyArc98534_1796.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000082 Genomic DNA. Translation: AAZ19372.1 .
RefSeqi YP_264806.1. NC_007204.1.

3D structure databases

ProteinModelPortali Q4FRI6.
SMRi Q4FRI6. Positions 13-372.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 259536.Psyc_1524.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ19372 ; AAZ19372 ; Psyc_1524 .
GeneIDi 3515485.
KEGGi par:Psyc_1524.
PATRICi 23057765. VBIPsyArc98534_1796.

Phylogenomic databases

eggNOGi COG0343.
HOGENOMi HOG000223473.
KOi K00773.
OMAi KYNSRSH.
OrthoDBi EOG6SNDVG.

Enzyme and pathway databases

UniPathwayi UPA00392 .
BioCyci PARC259536:GI3A-1560-MONOMER.

Family and domain databases

Gene3Di 3.20.20.105. 1 hit.
HAMAPi MF_00168. Q_tRNA_Tgt.
InterProi IPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view ]
PANTHERi PTHR11962. PTHR11962. 1 hit.
Pfami PF01702. TGT. 1 hit.
[Graphical view ]
SUPFAMi SSF51713. SSF51713. 1 hit.
TIGRFAMsi TIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Psychrobacter arcticus 273-4, a psychroactive Siberian permafrost bacterium, reveals mechanisms for adaptation to low-temperature growth."
    Ayala-del-Rio H.L., Chain P.S., Grzymski J.J., Ponder M.A., Ivanova N., Bergholz P.W., Di Bartolo G., Hauser L., Land M., Bakermans C., Rodrigues D., Klappenbach J., Zarka D., Larimer F., Richardson P., Murray A., Thomashow M., Tiedje J.M.
    Appl. Environ. Microbiol. 76:2304-2312(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 17307 / 273-4.

Entry informationi

Entry nameiTGT_PSYA2
AccessioniPrimary (citable) accession number: Q4FRI6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: August 30, 2005
Last modified: May 14, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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