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Q4FRI6 (TGT_PSYA2) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Queuine tRNA-ribosyltransferase

EC=2.4.2.29
Alternative name(s):
Guanine insertion enzyme
tRNA-guanine transglycosylase
Gene names
Name:tgt
Ordered Locus Names:Psyc_1524
OrganismPsychrobacter arcticus (strain DSM 17307 / 273-4) [Complete proteome] [HAMAP]
Taxonomic identifier259536 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter

Protein attributes

Sequence length383 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity. HAMAP-Rule MF_00168

Catalytic activity

Guanine34 in tRNA + queuine = queuosine34 in tRNA + guanine. HAMAP-Rule MF_00168

Guanine34 in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine34 in tRNA + guanine. HAMAP-Rule MF_00168

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00168

Pathway

tRNA modification; tRNA-queuosine biosynthesis. HAMAP-Rule MF_00168

Sequence similarities

Belongs to the queuine tRNA-ribosyltransferase family.

Ontologies

Keywords
   Biological processQueuosine biosynthesis
tRNA processing
   LigandMetal-binding
Zinc
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processqueuosine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

queuine tRNA-ribosyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 383383Queuine tRNA-ribosyltransferase HAMAP-Rule MF_00168
PRO_1000203659

Sites

Active site921Nucleophile By similarity
Metal binding3101Zinc By similarity
Metal binding3121Zinc By similarity
Metal binding3151Zinc By similarity
Metal binding3411Zinc By similarity
Binding site931Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4FRI6 [UniParc].

Last modified August 30, 2005. Version 1.
Checksum: D45D7218A5FD5FD0

FASTA38343,068
        10         20         30         40         50         60 
MQFVLHKTAS GVTRARRGTV HLNHGDVQTP AFMPVGTYGT VKGMLPRDIE AIGADIILGN 

        70         80         90        100        110        120 
TFHLWLRPGT DIIDKFGGLH KFMHWDKPIL TDSGGFQVFS LGAMRKITEE GVTFKSPIDG 

       130        140        150        160        170        180 
AKVFLSPEKS MQIQYSLNSD IVMQFDECTP YPATHDEAKK SLELSLRWGQ RCVDEHKKLG 

       190        200        210        220        230        240 
STNALFGIIQ GSMYADLRKQ SLEGLLEIGF DGYAIGGLSV GEPKEEMIDV LDYIANDMPA 

       250        260        270        280        290        300 
DKPRYLMGVG KPEDLVEGVR RGVDMFDCVM PTRNARNGHY FVTGDVDNAG VVRIRNSQYR 

       310        320        330        340        350        360 
TDEGPLDPEC DCYTCQNFSR AYLSHLNKCK EMLGAQLATI HNLRYYQRLM QGIRDAIEQD 

       370        380 
KFDEFVTEFY SKRGQTVPEL NLR 

« Hide

References

[1]"The genome sequence of Psychrobacter arcticus 273-4, a psychroactive Siberian permafrost bacterium, reveals mechanisms for adaptation to low-temperature growth."
Ayala-del-Rio H.L., Chain P.S., Grzymski J.J., Ponder M.A., Ivanova N., Bergholz P.W., Di Bartolo G., Hauser L., Land M., Bakermans C., Rodrigues D., Klappenbach J., Zarka D., Larimer F., Richardson P., Murray A., Thomashow M., Tiedje J.M.
Appl. Environ. Microbiol. 76:2304-2312(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 17307 / 273-4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000082 Genomic DNA. Translation: AAZ19372.1.
RefSeqYP_264806.1. NC_007204.1.

3D structure databases

ProteinModelPortalQ4FRI6.
SMRQ4FRI6. Positions 13-372.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING259536.Psyc_1524.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ19372; AAZ19372; Psyc_1524.
GeneID3515485.
KEGGpar:Psyc_1524.
PATRIC23057765. VBIPsyArc98534_1796.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0343.
HOGENOMHOG000223473.
KOK00773.
OMATPRFMPV.
OrthoDBEOG6SNDVG.
ProtClustDBPRK00112.

Enzyme and pathway databases

BioCycPARC259536:GI3A-1560-MONOMER.
UniPathwayUPA00392.

Family and domain databases

Gene3D3.20.20.105. 1 hit.
HAMAPMF_00168. Q_tRNA_Tgt.
InterProIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PANTHERPTHR11962. PTHR11962. 1 hit.
PfamPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMSSF51713. SSF51713. 1 hit.
TIGRFAMsTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTGT_PSYA2
AccessionPrimary (citable) accession number: Q4FRI6
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: August 30, 2005
Last modified: February 19, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways