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Q4FRI6

- TGT_PSYA2

UniProt

Q4FRI6 - TGT_PSYA2

Protein

Queuine tRNA-ribosyltransferase

Gene

tgt

Organism
Psychrobacter arcticus (strain DSM 17307 / 273-4)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (30 Aug 2005)
      Previous versions | rss
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    Functioni

    Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).UniRule annotation

    Catalytic activityi

    Guanine(34) in tRNA + queuine = queuosine(34) in tRNA + guanine.UniRule annotation
    Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine.UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei92 – 921NucleophileUniRule annotation
    Binding sitei93 – 931SubstrateUniRule annotation
    Metal bindingi310 – 3101ZincUniRule annotation
    Metal bindingi312 – 3121ZincUniRule annotation
    Metal bindingi315 – 3151ZincUniRule annotation
    Metal bindingi341 – 3411ZincUniRule annotation

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. queuine tRNA-ribosyltransferase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. queuosine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Queuosine biosynthesis, tRNA processing

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciPARC259536:GI3A-1560-MONOMER.
    UniPathwayiUPA00392.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Queuine tRNA-ribosyltransferaseUniRule annotation (EC:2.4.2.29UniRule annotation)
    Alternative name(s):
    Guanine insertion enzymeUniRule annotation
    tRNA-guanine transglycosylaseUniRule annotation
    Gene namesi
    Name:tgtUniRule annotation
    Ordered Locus Names:Psyc_1524
    OrganismiPsychrobacter arcticus (strain DSM 17307 / 273-4)
    Taxonomic identifieri259536 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter
    ProteomesiUP000000546: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 383383Queuine tRNA-ribosyltransferasePRO_1000203659Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi259536.Psyc_1524.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4FRI6.
    SMRiQ4FRI6. Positions 13-372.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the queuine tRNA-ribosyltransferase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0343.
    HOGENOMiHOG000223473.
    KOiK00773.
    OMAiKYNSRSH.
    OrthoDBiEOG6SNDVG.

    Family and domain databases

    Gene3Di3.20.20.105. 1 hit.
    HAMAPiMF_00168. Q_tRNA_Tgt.
    InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
    IPR002616. tRNA_ribo_trans-like.
    [Graphical view]
    PANTHERiPTHR11962. PTHR11962. 1 hit.
    PfamiPF01702. TGT. 1 hit.
    [Graphical view]
    SUPFAMiSSF51713. SSF51713. 1 hit.
    TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
    TIGR00449. tgt_general. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q4FRI6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQFVLHKTAS GVTRARRGTV HLNHGDVQTP AFMPVGTYGT VKGMLPRDIE    50
    AIGADIILGN TFHLWLRPGT DIIDKFGGLH KFMHWDKPIL TDSGGFQVFS 100
    LGAMRKITEE GVTFKSPIDG AKVFLSPEKS MQIQYSLNSD IVMQFDECTP 150
    YPATHDEAKK SLELSLRWGQ RCVDEHKKLG STNALFGIIQ GSMYADLRKQ 200
    SLEGLLEIGF DGYAIGGLSV GEPKEEMIDV LDYIANDMPA DKPRYLMGVG 250
    KPEDLVEGVR RGVDMFDCVM PTRNARNGHY FVTGDVDNAG VVRIRNSQYR 300
    TDEGPLDPEC DCYTCQNFSR AYLSHLNKCK EMLGAQLATI HNLRYYQRLM 350
    QGIRDAIEQD KFDEFVTEFY SKRGQTVPEL NLR 383
    Length:383
    Mass (Da):43,068
    Last modified:August 30, 2005 - v1
    Checksum:iD45D7218A5FD5FD0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000082 Genomic DNA. Translation: AAZ19372.1.
    RefSeqiYP_264806.1. NC_007204.1.

    Genome annotation databases

    EnsemblBacteriaiAAZ19372; AAZ19372; Psyc_1524.
    GeneIDi3515485.
    KEGGipar:Psyc_1524.
    PATRICi23057765. VBIPsyArc98534_1796.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000082 Genomic DNA. Translation: AAZ19372.1 .
    RefSeqi YP_264806.1. NC_007204.1.

    3D structure databases

    ProteinModelPortali Q4FRI6.
    SMRi Q4FRI6. Positions 13-372.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 259536.Psyc_1524.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAZ19372 ; AAZ19372 ; Psyc_1524 .
    GeneIDi 3515485.
    KEGGi par:Psyc_1524.
    PATRICi 23057765. VBIPsyArc98534_1796.

    Phylogenomic databases

    eggNOGi COG0343.
    HOGENOMi HOG000223473.
    KOi K00773.
    OMAi KYNSRSH.
    OrthoDBi EOG6SNDVG.

    Enzyme and pathway databases

    UniPathwayi UPA00392 .
    BioCyci PARC259536:GI3A-1560-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.105. 1 hit.
    HAMAPi MF_00168. Q_tRNA_Tgt.
    InterProi IPR004803. Queuine_tRNA-ribosylTrfase.
    IPR002616. tRNA_ribo_trans-like.
    [Graphical view ]
    PANTHERi PTHR11962. PTHR11962. 1 hit.
    Pfami PF01702. TGT. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51713. SSF51713. 1 hit.
    TIGRFAMsi TIGR00430. Q_tRNA_tgt. 1 hit.
    TIGR00449. tgt_general. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Psychrobacter arcticus 273-4, a psychroactive Siberian permafrost bacterium, reveals mechanisms for adaptation to low-temperature growth."
      Ayala-del-Rio H.L., Chain P.S., Grzymski J.J., Ponder M.A., Ivanova N., Bergholz P.W., Di Bartolo G., Hauser L., Land M., Bakermans C., Rodrigues D., Klappenbach J., Zarka D., Larimer F., Richardson P., Murray A., Thomashow M., Tiedje J.M.
      Appl. Environ. Microbiol. 76:2304-2312(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 17307 / 273-4.

    Entry informationi

    Entry nameiTGT_PSYA2
    AccessioniPrimary (citable) accession number: Q4FRI6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: August 30, 2005
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3