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Q4FRI6

- TGT_PSYA2

UniProt

Q4FRI6 - TGT_PSYA2

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Protein

Queuine tRNA-ribosyltransferase

Gene

tgt

Organism
Psychrobacter arcticus (strain DSM 17307 / 273-4)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).UniRule annotation

Catalytic activityi

Guanine(34) in tRNA + queuine = queuosine(34) in tRNA + guanine.UniRule annotation
Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine.UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei92 – 921NucleophileUniRule annotation
Binding sitei93 – 931SubstrateUniRule annotation
Metal bindingi310 – 3101ZincUniRule annotation
Metal bindingi312 – 3121ZincUniRule annotation
Metal bindingi315 – 3151ZincUniRule annotation
Metal bindingi341 – 3411ZincUniRule annotation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. queuine tRNA-ribosyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. queuosine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Queuosine biosynthesis, tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciPARC259536:GI3A-1560-MONOMER.
UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
Queuine tRNA-ribosyltransferaseUniRule annotation (EC:2.4.2.29UniRule annotation)
Alternative name(s):
Guanine insertion enzymeUniRule annotation
tRNA-guanine transglycosylaseUniRule annotation
Gene namesi
Name:tgtUniRule annotation
Ordered Locus Names:Psyc_1524
OrganismiPsychrobacter arcticus (strain DSM 17307 / 273-4)
Taxonomic identifieri259536 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter
ProteomesiUP000000546: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 383383Queuine tRNA-ribosyltransferasePRO_1000203659Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi259536.Psyc_1524.

Structurei

3D structure databases

ProteinModelPortaliQ4FRI6.
SMRiQ4FRI6. Positions 13-372.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the queuine tRNA-ribosyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0343.
HOGENOMiHOG000223473.
KOiK00773.
OMAiKYNSRSH.
OrthoDBiEOG6SNDVG.

Family and domain databases

Gene3Di3.20.20.105. 1 hit.
HAMAPiMF_00168. Q_tRNA_Tgt.
InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PANTHERiPTHR11962. PTHR11962. 1 hit.
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.

Sequencei

Sequence statusi: Complete.

Q4FRI6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQFVLHKTAS GVTRARRGTV HLNHGDVQTP AFMPVGTYGT VKGMLPRDIE
60 70 80 90 100
AIGADIILGN TFHLWLRPGT DIIDKFGGLH KFMHWDKPIL TDSGGFQVFS
110 120 130 140 150
LGAMRKITEE GVTFKSPIDG AKVFLSPEKS MQIQYSLNSD IVMQFDECTP
160 170 180 190 200
YPATHDEAKK SLELSLRWGQ RCVDEHKKLG STNALFGIIQ GSMYADLRKQ
210 220 230 240 250
SLEGLLEIGF DGYAIGGLSV GEPKEEMIDV LDYIANDMPA DKPRYLMGVG
260 270 280 290 300
KPEDLVEGVR RGVDMFDCVM PTRNARNGHY FVTGDVDNAG VVRIRNSQYR
310 320 330 340 350
TDEGPLDPEC DCYTCQNFSR AYLSHLNKCK EMLGAQLATI HNLRYYQRLM
360 370 380
QGIRDAIEQD KFDEFVTEFY SKRGQTVPEL NLR
Length:383
Mass (Da):43,068
Last modified:August 30, 2005 - v1
Checksum:iD45D7218A5FD5FD0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000082 Genomic DNA. Translation: AAZ19372.1.
RefSeqiYP_264806.1. NC_007204.1.

Genome annotation databases

EnsemblBacteriaiAAZ19372; AAZ19372; Psyc_1524.
GeneIDi3515485.
KEGGipar:Psyc_1524.
PATRICi23057765. VBIPsyArc98534_1796.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000082 Genomic DNA. Translation: AAZ19372.1 .
RefSeqi YP_264806.1. NC_007204.1.

3D structure databases

ProteinModelPortali Q4FRI6.
SMRi Q4FRI6. Positions 13-372.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 259536.Psyc_1524.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ19372 ; AAZ19372 ; Psyc_1524 .
GeneIDi 3515485.
KEGGi par:Psyc_1524.
PATRICi 23057765. VBIPsyArc98534_1796.

Phylogenomic databases

eggNOGi COG0343.
HOGENOMi HOG000223473.
KOi K00773.
OMAi KYNSRSH.
OrthoDBi EOG6SNDVG.

Enzyme and pathway databases

UniPathwayi UPA00392 .
BioCyci PARC259536:GI3A-1560-MONOMER.

Family and domain databases

Gene3Di 3.20.20.105. 1 hit.
HAMAPi MF_00168. Q_tRNA_Tgt.
InterProi IPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view ]
PANTHERi PTHR11962. PTHR11962. 1 hit.
Pfami PF01702. TGT. 1 hit.
[Graphical view ]
SUPFAMi SSF51713. SSF51713. 1 hit.
TIGRFAMsi TIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Psychrobacter arcticus 273-4, a psychroactive Siberian permafrost bacterium, reveals mechanisms for adaptation to low-temperature growth."
    Ayala-del-Rio H.L., Chain P.S., Grzymski J.J., Ponder M.A., Ivanova N., Bergholz P.W., Di Bartolo G., Hauser L., Land M., Bakermans C., Rodrigues D., Klappenbach J., Zarka D., Larimer F., Richardson P., Murray A., Thomashow M., Tiedje J.M.
    Appl. Environ. Microbiol. 76:2304-2312(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 17307 / 273-4.

Entry informationi

Entry nameiTGT_PSYA2
AccessioniPrimary (citable) accession number: Q4FRI6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: August 30, 2005
Last modified: October 1, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3