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Q4FR79 (ASSY_PSYA2) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Argininosuccinate synthase

EC=6.3.4.5
Alternative name(s):
Citrulline--aspartate ligase
Gene names
Name:argG
Ordered Locus Names:Psyc_1631
OrganismPsychrobacter arcticus (strain DSM 17307 / 273-4) [Complete proteome] [HAMAP]
Taxonomic identifier259536 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter

Protein attributes

Sequence length412 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP-Rule MF_00005

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP-Rule MF_00005

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00005

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00005.

Sequence similarities

Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processarginine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

argininosuccinate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 412412Argininosuccinate synthase HAMAP-Rule MF_00005
PRO_0000263958

Regions

Nucleotide binding15 – 239ATP By similarity

Sites

Binding site421ATP; via amide nitrogen and carbonyl oxygen By similarity
Binding site931Citrulline By similarity
Binding site981Citrulline By similarity
Binding site1231ATP; via amide nitrogen By similarity
Binding site1251Aspartate By similarity
Binding site1291Aspartate By similarity
Binding site1291Citrulline By similarity
Binding site1301Aspartate By similarity
Binding site1331Citrulline By similarity
Binding site1851Citrulline By similarity
Binding site1941Citrulline By similarity
Binding site2701Citrulline By similarity
Binding site2821Citrulline By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4FR79 [UniParc].

Last modified August 30, 2005. Version 1.
Checksum: EC1C1EC89EC98DEB

FASTA41246,197
        10         20         30         40         50         60 
MAQLDPKTIN KIVLAYSGGL DTSIIARWLQ ETYDAEVITF TADIGQGEEV EPARAKAEAM 

        70         80         90        100        110        120 
GIKHIHIEDL REEFARDYVF PMFRANAIYE GEYLLGTSIA RPLIAKRLVE IAKEHNADAI 

       130        140        150        160        170        180 
SHGATGKGND QVRFELGAVA LSPDVVTIAP WREWDLSSRE SLMEYAKEHN ISIDYAGNKK 

       190        200        210        220        230        240 
KSPYSMDANL LHISYEGGIL EDPYAEAEDD MWRWSVSPEQ APDVPQYLEL EYAKGDIVAI 

       250        260        270        280        290        300 
DGEALKPYEV MIKLNEIGGK HGIGRLDIVE NRYVGMKSRG CYETPAGTIM LKAHRGIESL 

       310        320        330        340        350        360 
TLDREAAHLK DELMPRYAKT IYNGYWFSPE RMMLQALIDK SQEYVNGTVR VKLYKGAVSV 

       370        380        390        400        410 
VGRKSDDSLF DEKIATFEDD AGAYDQKDAE GFIRLNGLRL AIEASRGRDL SK 

« Hide

References

[1]"The genome sequence of Psychrobacter arcticus 273-4, a psychroactive Siberian permafrost bacterium, reveals mechanisms for adaptation to low-temperature growth."
Ayala-del-Rio H.L., Chain P.S., Grzymski J.J., Ponder M.A., Ivanova N., Bergholz P.W., Di Bartolo G., Hauser L., Land M., Bakermans C., Rodrigues D., Klappenbach J., Zarka D., Larimer F., Richardson P., Murray A., Thomashow M., Tiedje J.M.
Appl. Environ. Microbiol. 76:2304-2312(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 17307 / 273-4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000082 Genomic DNA. Translation: AAZ19479.1.
RefSeqYP_264913.1. NC_007204.1.

3D structure databases

ProteinModelPortalQ4FR79.
SMRQ4FR79. Positions 11-404.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING259536.Psyc_1631.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ19479; AAZ19479; Psyc_1631.
GeneID3515598.
KEGGpar:Psyc_1631.
PATRIC23058033. VBIPsyArc98534_1924.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0137.
HOGENOMHOG000230093.
KOK01940.
OMAIYNGYWW.
OrthoDBEOG6K9QCV.
ProtClustDBPRK00509.

Enzyme and pathway databases

BioCycPARC259536:GI3A-1673-MONOMER.
UniPathwayUPA00068; UER00113.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPMF_00005. Arg_succ_synth_type1.
InterProIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsTIGR00032. argG. 1 hit.
PROSITEPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASSY_PSYA2
AccessionPrimary (citable) accession number: Q4FR79
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: August 30, 2005
Last modified: February 19, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways