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Q4FQX9 (TRMB_PSYA2) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:Psyc_1731
OrganismPsychrobacter arcticus (strain DSM 17307 / 273-4) [Complete proteome] [HAMAP]
Taxonomic identifier259536 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter

Protein attributes

Sequence length273 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 273273tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000229189

Regions

Region252 – 2554Substrate binding By similarity

Sites

Active site1791 By similarity
Binding site1051S-adenosyl-L-methionine By similarity
Binding site1301S-adenosyl-L-methionine By similarity
Binding site1571S-adenosyl-L-methionine By similarity
Binding site1791S-adenosyl-L-methionine By similarity
Binding site1831Substrate By similarity
Binding site2151Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4FQX9 [UniParc].

Last modified August 30, 2005. Version 1.
Checksum: 305762E325549D95

FASTA27330,788
        10         20         30         40         50         60 
MSQHPDINSS IDANHLTDDQ TSLDANAVID KEAMPDDSKH IRIVNTFMKR RTHMNKNAEL 

        70         80         90        100        110        120 
ALTAPEFAHY LVNNSFGDGN LDGIDDLRAL FADLPNGSDA PLTLEIGFGL GDSFIEMAAA 

       130        140        150        160        170        180 
EPSRNFVGVE VHEPGIGKCA YMAGTQNLSN IKIINGDAIQ LLKQLPENHI DRIQLYFPDP 

       190        200        210        220        230        240 
WQKKRHYKRR FVSAERMAIV TRSLKQGGWF HTATDWEHYA FWMVEVLDGF AGLSNQAGAG 

       250        260        270 
NFTDRPDFRP MTKFERRGLE RGHGVWDLIY LKD 

« Hide

References

[1]"The genome sequence of Psychrobacter arcticus 273-4, a psychroactive Siberian permafrost bacterium, reveals mechanisms for adaptation to low-temperature growth."
Ayala-del-Rio H.L., Chain P.S., Grzymski J.J., Ponder M.A., Ivanova N., Bergholz P.W., Di Bartolo G., Hauser L., Land M., Bakermans C., Rodrigues D., Klappenbach J., Zarka D., Larimer F., Richardson P., Murray A., Thomashow M., Tiedje J.M.
Appl. Environ. Microbiol. 76:2304-2312(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 17307 / 273-4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000082 Genomic DNA. Translation: AAZ19579.1.
RefSeqYP_265013.1. NC_007204.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING259536.Psyc_1731.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ19579; AAZ19579; Psyc_1731.
GeneID3515699.
KEGGpar:Psyc_1731.
PATRIC23058269. VBIPsyArc98534_2041.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMANSSIDAN.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycPARC259536:GI3A-1774-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_PSYA2
AccessionPrimary (citable) accession number: Q4FQX9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: August 30, 2005
Last modified: June 11, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways