Q4FQU7 (MDH_PSYA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	Psyc_1763

Organism

Psychrobacter arcticus (strain DSM 17307 / 273-4) [Complete proteome] [HAMAP]

Taxonomic identifier

259536 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Psychrobacter › Psychrobacter arcticus

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_01517
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_01517
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 2 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular_function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	Malate dehydrogenase HAMAP-Rule MF_01517		PRO_0000113389
Regions
	Nucleotide binding	13 – 19	7	NAD By similarity
	Nucleotide binding	131 – 133	3	NAD By similarity
Sites
	Active site	189	1	Proton acceptor By similarity
	Binding site	94	1	Substrate By similarity
	Binding site	100	1	Substrate By similarity
	Binding site	107	1	NAD By similarity
	Binding site	114	1	NAD By similarity
	Binding site	133	1	Substrate By similarity
	Binding site	164	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4FQU7 [UniParc]. Last modified August 30, 2005. Version 1. Checksum: 57C35528006AD4D9	FASTA	329	35,235
	10 20 30 40 50 60 MSMKQPVRVA VTGAAGNISY AMLFRIASGE MLGKDQPVIL QLLEIAPALD ALKGVVMELE 70 80 90 100 110 120 DCAFPLLAGI VQTDDATVAF KDVDYALLVG SRPRGPGMER KDLLEANAAI FSAQGKALND 130 140 150 160 170 180 VASRDVKVLV VGNPANTNAL IAQRNAPDLD PRNFTAMTRL DHNRAMAQLA GKTDSTVNDV 190 200 210 220 230 240 KKMIIWGNHS STQYPDLTAS TVNGKLALDL VDRTWYEGTY IPEVQQRGAA IIKARGASSA 250 260 270 280 290 300 ASAANAAIAH MRTWVLGTDE NDWVSMGVYS NGEYGIAKGL IYSFPCTCTN GDWSIVDGVD 310 320 VSSAFSKEKM AATEQELSEE RDAVAHLLP

References

[1]

"The genome sequence of Psychrobacter arcticus 273-4, a psychroactive Siberian permafrost bacterium, reveals mechanisms for adaptation to low-temperature growth." Ayala-del-Rio H.L., Chain P.S., Grzymski J.J., Ponder M.A., Ivanova N., Bergholz P.W., Di Bartolo G., Hauser L., Land M., Bakermans C., Rodrigues D., Klappenbach J., Zarka D., Larimer F., Richardson P., Murray A., Thomashow M., Tiedje J.M. Appl. Environ. Microbiol. 76:2304-2312(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 17307 / 273-4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000082 Genomic DNA. Translation: AAZ19611.1.
RefSeq	YP_265045.1. NC_007204.1.
3D structure databases
ProteinModelPortal	Q4FQU7.
SMR	Q4FQU7. Positions 3-327.
ModBase	Search...
Protein-protein interaction databases
STRING	259536.Psyc_1763.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAZ19611; AAZ19611; Psyc_1763.
GeneID	3515731.
KEGG	par:Psyc_1763.
PATRIC	23058341. VBIPsyArc98534_2077.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0039.
HOGENOM	HOG000220953.
KO	K00024.
OMA	NCLIASK.
ProtClustDB	PRK05442.
Enzyme and pathway databases
BioCyc	PARC259536:GI3A-1828-MONOMER.
Family and domain databases
Gene3D	3.40.50.720. 1 hit. 3.90.110.10. 1 hit.
HAMAP	MF_01517. Malate_dehydrog_2.
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR001252. Malate_DH_AS. IPR010945. Malate_DH_type2. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR23382. PTHR23382. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01759. MalateDH-SF1. 1 hit.
PROSITE	PS00068. MDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDH_PSYA2

Accession

Primary (citable) accession number: Q4FQU7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	August 30, 2005
Last modified:	May 1, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program