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Q4FQJ9 (BIOB_PSYA2) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Psyc_1861
OrganismPsychrobacter arcticus (strain DSM 17307 / 273-4) [Complete proteome] [HAMAP]
Taxonomic identifier259536 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter

Protein attributes

Sequence length378 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 378378Biotin synthase HAMAP-Rule MF_01694
PRO_0000381568

Sites

Metal binding831Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding871Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding901Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1291Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1601Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2201Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2961Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4FQJ9 [UniParc].

Last modified August 30, 2005. Version 1.
Checksum: 85114A8448C5911B

FASTA37841,377
        10         20         30         40         50         60 
MSALLNITEP NNNTNTKNDH FLASLTTDAT QSTASKYSRE QIAQLFDLPL MDLLLQAQTI 

        70         80         90        100        110        120 
HRQNFTANEV QISTLLSIKT GNCPEDCGYC SQSGHHRDKT ELVAEKRIEV SKVIAAAKRA 

       130        140        150        160        170        180 
KATGSSRFCM GAAWKHPSAK DMPYVVELVK EVKALGLETC MTLGMLDTDQ AAQLANAGLD 

       190        200        210        220        230        240 
YYNHNLDTSR SYYEQVVSTR SYDERLDTIA NVRNSGINVC SGNIVGMGES RDDRIDWVHE 

       250        260        270        280        290        300 
LLKMPKAPES IPVNLLVPIQ GTPIGDKVLA EGQLSVLEWI RTIAVTRICC PSSYVRLSAG 

       310        320        330        340        350        360 
RESLSDAEQA LAFMAGANSF FYGDKLLTTG NASQSGDDRL MRELGLTKQF SAPRLPKQVP 

       370 
VLDAMSGHQS QVILASLS 

« Hide

References

[1]"The genome sequence of Psychrobacter arcticus 273-4, a psychroactive Siberian permafrost bacterium, reveals mechanisms for adaptation to low-temperature growth."
Ayala-del-Rio H.L., Chain P.S., Grzymski J.J., Ponder M.A., Ivanova N., Bergholz P.W., Di Bartolo G., Hauser L., Land M., Bakermans C., Rodrigues D., Klappenbach J., Zarka D., Larimer F., Richardson P., Murray A., Thomashow M., Tiedje J.M.
Appl. Environ. Microbiol. 76:2304-2312(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 17307 / 273-4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000082 Genomic DNA. Translation: AAZ19709.1.
RefSeqYP_265143.1. NC_007204.1.

3D structure databases

ProteinModelPortalQ4FQJ9.
SMRQ4FQJ9. Positions 36-349.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING259536.Psyc_1861.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ19709; AAZ19709; Psyc_1861.
GeneID3515830.
KEGGpar:Psyc_1861.
PATRIC23058591. VBIPsyArc98534_2201.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMAADRFCMG.
OrthoDBEOG622PMP.
ProtClustDBCLSK2391056.

Enzyme and pathway databases

BioCycPARC259536:GI3A-1905-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_PSYA2
AccessionPrimary (citable) accession number: Q4FQJ9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: August 30, 2005
Last modified: February 19, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways