Reviewed, UniProtKB/Swiss-Prot Q4FQC6 (FADB_PSYA2)

Names and origin

Protein names

Recommended name:
    Fatty acid oxidation complex subunit alpha
Including the following 2 domains:
    1- Recommended name:
            Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase
              EC=4.2.1.17
              EC=5.3.3.8
              EC=5.1.2.3
    2- Recommended name:
            3-hydroxyacyl-CoA dehydrogenase
              EC=1.1.1.35

Gene names

Name:	fadB
Ordered Locus Names:	Psyc_1934

Organism

Psychrobacter arcticus (strain DSM 17307 / 273-4) [Complete proteome] [HAMAP]

Taxonomic identifier

259536 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Psychrobacter

Protein attributes

Sequence length	719 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of an hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities By similarity. HAMAP MF_01621
Catalytic activity	(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH. HAMAP MF_01621 (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O. HAMAP MF_01621 (S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA. HAMAP MF_01621 (3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA. HAMAP MF_01621
Pathway	Lipid metabolism; fatty acid beta-oxidation. HAMAP MF_01621
Subunit structure	Heterotetramer of two alpha chains (fadB) and two beta chains (fadA) By similarity. HAMAP MF_01621
Sequence similarities	In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family. In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid degradation Lipid metabolism
Ligand	NAD
Molecular function	Isomerase Lyase Oxidoreductase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	fatty acid catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	fatty acid beta-oxidation multienzyme complex Inferred from electronic annotation. Source: InterPro
Molecular function	3-hydroxyacyl-CoA dehydrogenase activity Inferred from electronic annotation. Source: HAMAP 3-hydroxybutyryl-CoA epimerase activity Inferred from electronic annotation. Source: HAMAP coenzyme binding Inferred from electronic annotation. Source: InterPro dodecenoyl-CoA delta-isomerase activity Inferred from electronic annotation. Source: HAMAP enoyl-CoA hydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 719	719	Fatty acid oxidation complex subunit alpha HAMAP MF_01621		PRO_0000109283
Regions
	Nucleotide binding	402 – 404	3	NAD By similarity
	Nucleotide binding	429 – 431	3	NAD By similarity
	Region	1 – 190	190	Enoyl-CoA hydratase/isomerase By similarity
	Region	313 – 719	407	3-hydroxyacyl-CoA dehydrogenase By similarity
Sites
	Binding site	298	1	Substrate By similarity
	Binding site	326	1	NAD; via amide nitrogen By similarity
	Binding site	345	1	NAD By similarity
	Binding site	409	1	NAD By similarity
	Binding site	455	1	NAD By similarity
	Binding site	502	1	Substrate By similarity
	Site	120	1	Important for catalytic activity By similarity
	Site	140	1	Important for catalytic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4FQC6-1 [UniParc]. Last modified August 30, 2005. Version 1. Checksum: 67B55A992065C896	FASTA	719	78,225
	10 20 30 40 50 60 MVYQGNRITV TMLEDGIANM QYNAENESVN KFDTETNKQF AEVVNALEKA DDVKGLIVTS 70 80 90 100 110 120 SKGVFIAGAD ITEFVASFKQ SEEEIKDWVI NINDAFNRFE DLPFPKVAAI NGAALGGGCE 130 140 150 160 170 180 MTLVCEYRVM SDKAIIGLPE TQLGIFPGFG GTVRSTRVIG IDNALELIAT GAPKKALDAL 190 200 210 220 230 240 KLGLVDATVA ADDLQDAAID LVKKCISGEL DWKAKREEKL VAVKLNQLEQ AMAFNSAKGM 250 260 270 280 290 300 IFAKANPKQY PAPALAIAAI EKHVNLPRDK AIEVEAAGFA KAAKTPQAES LVGLFLSDQL 310 320 330 340 350 360 VKKLAKQHSK KAHEINEAAV LGAGIMGGGI AYQAASKGLP IIMKDIKSEQ LDLGMGEASK 370 380 390 400 410 420 LLGKMVDRGK MTPAKMGETL SRIRPTLNYG DFAETDIVIE AVVENPNVKR AVLKEVEGLV 430 440 450 460 470 480 KDDCILASNT STISITFLAE ALERPENFVG MHFFNPVHRM PLVEVIRGEK SSEEAIATTV 490 500 510 520 530 540 ALASKMGKVP VVVNDCPGFL VNRVLFPYFG AFDLLLKQGA DFAHVDKVME KFGWPMGPAY 550 560 570 580 590 600 LIDVVGLDTG VHGAEVMAEG FPDRMKPDYK GAIELLYENK RLGQKNGVGF YKYEMDKRGK 610 620 630 640 650 660 PKKVADEATY ELLKTTTDSE KQTFDDQAII DRTMLAFCNE TVRCLEDNIV STPSEADMAM 670 680 690 700 710 IMGVGFPPFR GGPCRYIDQM GLDNYLALCE KYAHLGKAYE APQKIRDMAA AGETFYATA

References

[1]

"Complete sequence of Psychrobacter arcticum 273-4." NASA Astrobiology Institute Ayala-del-Rio H.L., Chain P., Ponder M.A., Di Bartolo G., Ivanova N., Bergholz P.W., Hauser L., Land M., Bakermans C., Rodrigues D., Klappenbach J.A., Zarka D., Larimer F., Richardson P., Thomashow M.F., Tiedje J.M. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000082 Genomic DNA. Translation: AAZ19782.1.
RefSeq	YP_265216.1.
3D structure databases
SMR	Q4FQC6. Positions 1-717.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4FQC6.
Genome annotation databases
GeneID	3515904.
GenomeReviews	Gene locus Psyc_1934 in contig CP000082_GR.
KEGG	par:Psyc_1934.
NMPDR	fig|259536.4.peg.1754.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1250.
HOGENOM	HBG691737.
OMA	ASRFGQK.
PhylomeDB	Q4FQC6.
Enzyme and pathway databases
BioCyc	PARC259536:PSYC_1934-MONOMER.
Family and domain databases
HAMAP	MF_01621. FadB. [Tree]
InterPro	IPR006180. 3-OHacyl-CoA_DH_CS. IPR006176. 3-OHacyl-CoA_DH_NAD-bd. IPR006108. 3HC_DH_C. IPR008927. 6-PGluconate_DH_C-like. IPR001753. Crotonase_core. IPR013328. DH_multihelical. IPR012799. FadB. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
Pfam	PF00725. 3HCDH. 1 hit. PF02737. 3HCDH_N. 1 hit. PF00378. ECH. 1 hit. [Graphical view]
TIGRFAMs	TIGR02437. FadB. 1 hit.
PROSITE	PS00067. 3HCDH. 1 hit. PS00166. ENOYL_COA_HYDRATASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FADB_PSYA2

Accession

Primary (citable) accession number: Q4FQC6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	August 30, 2005
Last modified:	February 9, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)