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Q4FPK8 (DDL_PELUB) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:SAR11_0021
OrganismPelagibacter ubique (strain HTCC1062) [Complete proteome] [HAMAP]
Taxonomic identifier335992 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaSAR11 clusterCandidatus Pelagibacter

Protein attributes

Sequence length304 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 304304D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341145

Regions

Domain101 – 298198ATP-grasp
Nucleotide binding131 – 18454ATP By similarity

Sites

Metal binding2531Magnesium or manganese 1 By similarity
Metal binding2651Magnesium or manganese 1 By similarity
Metal binding2651Magnesium or manganese 2 By similarity
Metal binding2671Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4FPK8 [UniParc].

Last modified August 30, 2005. Version 1.
Checksum: 7752A1154105E5B1

FASTA30434,397
        10         20         30         40         50         60 
MTKKILILSG GISKERLISL DTGKQVAKEL IKNGYKVLIS EPDKNLSKNI TSFKPDVIFN 

        70         80         90        100        110        120 
ALHGQFGEDG YIQAILETKK IPYTHSGVIA SSIAMDKEIS KKIFIKNKIL TPKYIKFNHK 

       130        140        150        160        170        180 
KNKLNIIKLI EKNLKFPVVV KPINEGSSVH VYICDKTNIL KNLKVLKSYN EILIEEFIPG 

       190        200        210        220        230        240 
REIQVAIMNN KSLGAIELEP RRKFYDYEAK YNSSAKTKHL IPVDLSKNNL AKITGIARAA 

       250        260        270        280        290        300 
HKIIGCKGVT RSDFKFFNGK FYLLEINTQP GMTKLSLVPE IAKHKGISFI KLIEWILKDA 


SINR 

« Hide

References

[1]"Genome streamlining in a cosmopolitan oceanic bacterium."
Giovannoni S.J., Tripp H.J., Givan S., Podar M., Vergin K.L., Baptista D., Bibbs L., Eads J., Richardson T.H., Noordewier M., Rappe M.S., Short J.M., Carrington J.C., Mathur E.J.
Science 309:1242-1245(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HTCC1062.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000084 Genomic DNA. Translation: AAZ20846.1.
RefSeqYP_265449.1. NC_007205.1.

3D structure databases

ProteinModelPortalQ4FPK8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING335992.SAR11_0021.

Proteomic databases

PRIDEQ4FPK8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ20846; AAZ20846; SAR11_0021.
GeneID3516651.
KEGGpub:SAR11_0021.
PATRIC31989497. VBICanPel5618_0022.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMAHEIFTIS.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycCPEL335992:GH3Z-21-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_PELUB
AccessionPrimary (citable) accession number: Q4FPK8
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: August 30, 2005
Last modified: February 19, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways