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Q4FPA9

- SYI_PELUB

UniProt

Q4FPA9 - SYI_PELUB

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Protein

Isoleucine--tRNA ligase

Gene
ileS, SAR11_0158
Organism
Pelagibacter ubique (strain HTCC1062)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei563 – 5631Aminoacyl-adenylate By similarity
Binding sitei607 – 6071ATP By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCPEL335992:GH3Z-158-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:SAR11_0158
OrganismiPelagibacter ubique (strain HTCC1062)
Taxonomic identifieri335992 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSAR11 clusterCandidatus Pelagibacter
ProteomesiUP000002528: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 905905Isoleucine--tRNA ligaseUniRule annotationPRO_0000098435Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi335992.SAR11_0158.

Structurei

3D structure databases

ProteinModelPortaliQ4FPA9.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi56 – 6611"HIGH" regionUniRule annotationAdd
BLAST
Motifi604 – 6085"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4FPA9-1 [UniParc]FASTAAdd to Basket

« Hide

MSKENINLPK TAFSMKANLQ NKEPEILEYW KKIDLYKELR KSNKGKEKFI    50
LHDGPPYANG NIHMGTALNK ILKDIIVKFH QMDGKDSVYV PGWDCHGLPI 100
EWKIEEQYKK NKKNKNEVPI TEFRKECREF AEKWIEVHKT QFKRLGVVGD 150
WENHYATMSF EAEAQIVREL GKFLKEGSLY RGFKPVLWST VEKTALADAE 200
VEYQDHKSDT IYTAFPVKKT NIKELENTDV IIWTTTPWTI PANKALAYNE 250
ALDYLIIELG DEGDFKNKKI VVAEALLESV IKDCEIKSYK EIKKFKGKDF 300
KDTICSHPFL ELGYDYDIPM LEARFVTTEQ GTGIVHCAPS HGPDDFNLCL 350
KYGIKAIETV DGDGKYTKNL PLFEGTHIFK ANPIVIEKLK EQKKLLSNGE 400
LVHSYPHSWR SKAPLVHRAT PQWFISMESH GLRKKALKAL DDTKFYPDKG 450
RERIKAMIET RPDWCVSRQR VWGVPLPIFV HKTTKEILVD DNVNENIASI 500
YEKEGSDCWF SDSPQRFLGD KYKAEDYEKI SDIVEVWFDS GCTHAFVLEK 550
REDLQWPASM YLEGSDQHRG WFHSSLLESC GTRGRAPYES ILSHGFVVDG 600
KGLKMSKSVG NVIAPEDILK KYGADILRIW VASSNYAEDL RIDYSILEQH 650
ADSYRKIRNT FRYLLGNLND DFQKIDLESL DIKQLPELER YMLHRVYELN 700
NNFKNYFKSY DFHNLYKELL NFCTVDLSSF YFDIRKDALY CDSKNSDRRK 750
SSIVVLNIIL ESLVKWFAPI LSFTTEEIFT LINKEQKSIH LEQFMKYPES 800
FQDEELHKKW IELKKIRDIC NISIEAKRAS KEIGSSLEAS LIINLNKSLF 850
EISKNVDFSE ICITSSALVH QSNTDEIIIK TIKAEGNKCP VCWKINKVKC 900
ERHSD 905
Length:905
Mass (Da):105,047
Last modified:August 30, 2005 - v1
Checksum:iB8CD6E7541EDEF57
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000084 Genomic DNA. Translation: AAZ20980.1.
RefSeqiWP_006997752.1. NC_007205.1.
YP_265583.1. NC_007205.1.

Genome annotation databases

EnsemblBacteriaiAAZ20980; AAZ20980; SAR11_0158.
GeneIDi3516865.
KEGGipub:SAR11_0158.
PATRICi31989773. VBICanPel5618_0157.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000084 Genomic DNA. Translation: AAZ20980.1 .
RefSeqi WP_006997752.1. NC_007205.1.
YP_265583.1. NC_007205.1.

3D structure databases

ProteinModelPortali Q4FPA9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 335992.SAR11_0158.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ20980 ; AAZ20980 ; SAR11_0158 .
GeneIDi 3516865.
KEGGi pub:SAR11_0158.
PATRICi 31989773. VBICanPel5618_0157.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CPEL335992:GH3Z-158-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HTCC1062.

Entry informationi

Entry nameiSYI_PELUB
AccessioniPrimary (citable) accession number: Q4FPA9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: August 30, 2005
Last modified: September 3, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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