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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Pelagibacter ubique (strain HTCC1062)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei563Aminoacyl-adenylateUniRule annotation1
Binding sitei607ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:SAR11_0158
OrganismiPelagibacter ubique (strain HTCC1062)
Taxonomic identifieri335992 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaPelagibacteralesPelagibacteraceaeCandidatus Pelagibacter
Proteomesi
  • UP000002528 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000984351 – 905Isoleucine--tRNA ligaseAdd BLAST905

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi335992.SAR11_0158

Structurei

3D structure databases

ProteinModelPortaliQ4FPA9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi56 – 66"HIGH" regionAdd BLAST11
Motifi604 – 608"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q4FPA9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKENINLPK TAFSMKANLQ NKEPEILEYW KKIDLYKELR KSNKGKEKFI
60 70 80 90 100
LHDGPPYANG NIHMGTALNK ILKDIIVKFH QMDGKDSVYV PGWDCHGLPI
110 120 130 140 150
EWKIEEQYKK NKKNKNEVPI TEFRKECREF AEKWIEVHKT QFKRLGVVGD
160 170 180 190 200
WENHYATMSF EAEAQIVREL GKFLKEGSLY RGFKPVLWST VEKTALADAE
210 220 230 240 250
VEYQDHKSDT IYTAFPVKKT NIKELENTDV IIWTTTPWTI PANKALAYNE
260 270 280 290 300
ALDYLIIELG DEGDFKNKKI VVAEALLESV IKDCEIKSYK EIKKFKGKDF
310 320 330 340 350
KDTICSHPFL ELGYDYDIPM LEARFVTTEQ GTGIVHCAPS HGPDDFNLCL
360 370 380 390 400
KYGIKAIETV DGDGKYTKNL PLFEGTHIFK ANPIVIEKLK EQKKLLSNGE
410 420 430 440 450
LVHSYPHSWR SKAPLVHRAT PQWFISMESH GLRKKALKAL DDTKFYPDKG
460 470 480 490 500
RERIKAMIET RPDWCVSRQR VWGVPLPIFV HKTTKEILVD DNVNENIASI
510 520 530 540 550
YEKEGSDCWF SDSPQRFLGD KYKAEDYEKI SDIVEVWFDS GCTHAFVLEK
560 570 580 590 600
REDLQWPASM YLEGSDQHRG WFHSSLLESC GTRGRAPYES ILSHGFVVDG
610 620 630 640 650
KGLKMSKSVG NVIAPEDILK KYGADILRIW VASSNYAEDL RIDYSILEQH
660 670 680 690 700
ADSYRKIRNT FRYLLGNLND DFQKIDLESL DIKQLPELER YMLHRVYELN
710 720 730 740 750
NNFKNYFKSY DFHNLYKELL NFCTVDLSSF YFDIRKDALY CDSKNSDRRK
760 770 780 790 800
SSIVVLNIIL ESLVKWFAPI LSFTTEEIFT LINKEQKSIH LEQFMKYPES
810 820 830 840 850
FQDEELHKKW IELKKIRDIC NISIEAKRAS KEIGSSLEAS LIINLNKSLF
860 870 880 890 900
EISKNVDFSE ICITSSALVH QSNTDEIIIK TIKAEGNKCP VCWKINKVKC

ERHSD
Length:905
Mass (Da):105,047
Last modified:August 30, 2005 - v1
Checksum:iB8CD6E7541EDEF57
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000084 Genomic DNA Translation: AAZ20980.1
RefSeqiWP_006997752.1, NC_007205.1

Genome annotation databases

EnsemblBacteriaiAAZ20980; AAZ20980; SAR11_0158
KEGGipub:SAR11_0158

Similar proteinsi

Entry informationi

Entry nameiSYI_PELUB
AccessioniPrimary (citable) accession number: Q4FPA9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: August 30, 2005
Last modified: March 28, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health