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Q4FPA9

- SYI_PELUB

UniProt

Q4FPA9 - SYI_PELUB

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Pelagibacter ubique (strain HTCC1062)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (30 Aug 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei563 – 5631Aminoacyl-adenylateUniRule annotation
    Binding sitei607 – 6071ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciCPEL335992:GH3Z-158-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:SAR11_0158
    OrganismiPelagibacter ubique (strain HTCC1062)
    Taxonomic identifieri335992 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSAR11 clusterCandidatus Pelagibacter
    ProteomesiUP000002528: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 905905Isoleucine--tRNA ligasePRO_0000098435Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi335992.SAR11_0158.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4FPA9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi56 – 6611"HIGH" regionAdd
    BLAST
    Motifi604 – 6085"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q4FPA9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKENINLPK TAFSMKANLQ NKEPEILEYW KKIDLYKELR KSNKGKEKFI    50
    LHDGPPYANG NIHMGTALNK ILKDIIVKFH QMDGKDSVYV PGWDCHGLPI 100
    EWKIEEQYKK NKKNKNEVPI TEFRKECREF AEKWIEVHKT QFKRLGVVGD 150
    WENHYATMSF EAEAQIVREL GKFLKEGSLY RGFKPVLWST VEKTALADAE 200
    VEYQDHKSDT IYTAFPVKKT NIKELENTDV IIWTTTPWTI PANKALAYNE 250
    ALDYLIIELG DEGDFKNKKI VVAEALLESV IKDCEIKSYK EIKKFKGKDF 300
    KDTICSHPFL ELGYDYDIPM LEARFVTTEQ GTGIVHCAPS HGPDDFNLCL 350
    KYGIKAIETV DGDGKYTKNL PLFEGTHIFK ANPIVIEKLK EQKKLLSNGE 400
    LVHSYPHSWR SKAPLVHRAT PQWFISMESH GLRKKALKAL DDTKFYPDKG 450
    RERIKAMIET RPDWCVSRQR VWGVPLPIFV HKTTKEILVD DNVNENIASI 500
    YEKEGSDCWF SDSPQRFLGD KYKAEDYEKI SDIVEVWFDS GCTHAFVLEK 550
    REDLQWPASM YLEGSDQHRG WFHSSLLESC GTRGRAPYES ILSHGFVVDG 600
    KGLKMSKSVG NVIAPEDILK KYGADILRIW VASSNYAEDL RIDYSILEQH 650
    ADSYRKIRNT FRYLLGNLND DFQKIDLESL DIKQLPELER YMLHRVYELN 700
    NNFKNYFKSY DFHNLYKELL NFCTVDLSSF YFDIRKDALY CDSKNSDRRK 750
    SSIVVLNIIL ESLVKWFAPI LSFTTEEIFT LINKEQKSIH LEQFMKYPES 800
    FQDEELHKKW IELKKIRDIC NISIEAKRAS KEIGSSLEAS LIINLNKSLF 850
    EISKNVDFSE ICITSSALVH QSNTDEIIIK TIKAEGNKCP VCWKINKVKC 900
    ERHSD 905
    Length:905
    Mass (Da):105,047
    Last modified:August 30, 2005 - v1
    Checksum:iB8CD6E7541EDEF57
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000084 Genomic DNA. Translation: AAZ20980.1.
    RefSeqiWP_006997752.1. NC_007205.1.
    YP_265583.1. NC_007205.1.

    Genome annotation databases

    EnsemblBacteriaiAAZ20980; AAZ20980; SAR11_0158.
    GeneIDi3516865.
    KEGGipub:SAR11_0158.
    PATRICi31989773. VBICanPel5618_0157.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000084 Genomic DNA. Translation: AAZ20980.1 .
    RefSeqi WP_006997752.1. NC_007205.1.
    YP_265583.1. NC_007205.1.

    3D structure databases

    ProteinModelPortali Q4FPA9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 335992.SAR11_0158.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAZ20980 ; AAZ20980 ; SAR11_0158 .
    GeneIDi 3516865.
    KEGGi pub:SAR11_0158.
    PATRICi 31989773. VBICanPel5618_0157.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci CPEL335992:GH3Z-158-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HTCC1062.

    Entry informationi

    Entry nameiSYI_PELUB
    AccessioniPrimary (citable) accession number: Q4FPA9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: August 30, 2005
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3