Q4FLS1 (DAPA_PELUB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydrodipicolinate synthase
Short name=DHDPS
EC=4.2.1.52

Gene names

Name:	dapA
Ordered Locus Names:	SAR11_1062

Organism

Pelagibacter ubique (strain HTCC1062) [Complete proteome] [HAMAP]

Taxonomic identifier

335992 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › SAR11 cluster › Candidatus Pelagibacter

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418
Subunit structure	Homotetramer By similarity. HAMAP MF_00418
Subcellular location	Cytoplasm By similarity HAMAP MF_00418.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 293	293	Dihydrodipicolinate synthase HAMAP MF_00418		PRO_1000050239
Regions
	Region	47 – 48	2	Pyruvate binding By similarity
Sites
	Active site	160	1	Schiff-base intermediate with substrate By similarity
	Binding site	105	1	Pyruvate By similarity
	Site	132	1	Involved in proton transfer during cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4FLS1 [UniParc]. Last modified August 30, 2005. Version 1. Checksum: 328B74D978F76A2E	FASTA	293	31,783
	10 20 30 40 50 60 MFKGSNVALI TPFKNNELDV EAYIKLIHFQ ISNGTNGLVP AGTTGESPTL SHDEHQKVID 70 80 90 100 110 120 LCVKESNGKN IVIAGTGSNS TKEAISLTTH AEKAGADAAL IVTPYYNKPT QEGLYQHYKA 130 140 150 160 170 180 INDKCGIPII IYNIPGRSVI DMSVDTMARL FELKNIVGVK DATGDLDRVN QQKDKMGTDF 190 200 210 220 230 240 VQLTGNDDNA LEFNNRGGVG SISVTANIAP KLCSDFQKAS KIENNESKLE AIRLDKILQP 250 260 270 280 290 IHSSMFIESN PSPVKYAAKL LGLCDDEVRL PLVKVTDPTK DIVKEALVSA NLL

References

[1]

"Genome streamlining in a cosmopolitan oceanic bacterium." Giovannoni S.J., Tripp H.J., Givan S., Podar M., Vergin K.L., Baptista D., Bibbs L., Eads J., Richardson T.H., Noordewier M., Rappe M.S., Short J.M., Carrington J.C., Mathur E.J. Science 309:1242-1245(2005) [PubMed: 16109880] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HTCC1062.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000084 Genomic DNA. Translation: AAZ21867.1.
RefSeq	YP_266471.1. NC_007205.1.
3D structure databases
HSSP	HSSP built from PDB template 2A6N based on UniProtKB P0A6L2.
ProteinModelPortal	Q4FLS1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3517401.
GenomeReviews	Gene locus SAR11_1062 in contig CP000084_GR.
KEGG	pub:SAR11_1062.
PATRIC	31991611. VBICanPel5618_1052.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0329.
HOGENOM	HBG358848.
OMA	HGCISVV.
PhylomeDB	Q4FLS1.
ProtClustDB	PRK03170.
Enzyme and pathway databases
BioCyc	CPEL335992:SAR11_1062-MONOMER.
Family and domain databases
HAMAP	MF_00418. DapA. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR002220. Dihydrodipicolinate_synth-like. IPR020625. Dihydrodipicolinate_synth_AS. IPR020624. Dihydrodipicolinate_synth_CS. IPR005263. Dihydrodipicolinate_synth_DapA. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01714.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PIRSF	PIRSF001365. DHDPS. 1 hit.
PRINTS	PR00146. DHPICSNTHASE.
TIGRFAMs	TIGR00674. DapA. 1 hit.
PROSITE	PS00665. DHDPS_1. 1 hit. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA_PELUB

Accession

Primary (citable) accession number: Q4FLS1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 30, 2005
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program