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Protein

Serine/threonine-protein kinase SSN3

Gene

SSN3

Organism
Gibberella moniliformis (Maize ear and stalk rot fungus) (Fusarium verticillioides)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the SRB8-11 complex. The SRB8-11 complex is a regulatory module of the Mediator complex which is itself involved in regulation of basal and activated RNA polymerase II-dependent transcription. The SRB8-11 complex may be involved in the transcriptional repression of a subset of genes regulated by Mediator. It may inhibit the association of the Mediator complex with RNA polymerase II to form the holoenzyme complex. The SRB8-11 complex phosphorylates the C-terminal domain (CTD) of the largest subunit of RNA polymerase II (By similarity). Required for normal growth and secondary metabolism.By similarity1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei71 – 711ATPPROSITE-ProRule annotation
Active sitei173 – 1731Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi46 – 549ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
  4. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: UniProtKB-KW
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Kinase, Repressor, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase SSN3 (EC:2.7.11.22, EC:2.7.11.23)
Alternative name(s):
Cyclin-dependent kinase 8
Gene namesi
Name:SSN3
Synonyms:CDK8, FCK1
OrganismiGibberella moniliformis (Maize ear and stalk rot fungus) (Fusarium verticillioides)
Taxonomic identifieri117187 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesNectriaceaeFusariumFusarium fujikuroi species complex

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 450450Serine/threonine-protein kinase SSN3PRO_0000312945Add
BLAST

Interactioni

Subunit structurei

Component of the SRB8-11 complex, a regulatory module of the Mediator complex (By similarity). Interacts with SSN8/FCC1.By similarity1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ4FCZ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 393354Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi310 – 32516His-richAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4FCZ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLRPHIGLG FPAHAYQKRH VEPHDRSTGY QPKVRITDRY RIIGFISSGT
60 70 80 90 100
YGRVYKAVGR NGKPVGEFAI KKFKPDKEGE QISYTGISQS AIREMSLCSE
110 120 130 140 150
LHHINVIRLC EIVLEDKCIF MVFEYAEHDL LQIIHHHTQQ PRHPIPPATI
160 170 180 190 200
KSIMFQLLNG CQYLHINWVL HRDLKPANIM VTSSGEVKIG DLGLARRFDK
210 220 230 240 250
PLHSLFSGDK VVVTIWYRAP ELILGSYHYT PAIDMWAVGC IFAELLSLRP
260 270 280 290 300
IFKGEEAKMD SKKTVPFRRN QMQKIIEIMG VPTKDKWPLL STMPEYNQLN
310 320 330 340 350
TLANSMASSH HNHHSHHHPH HHHGHYGSRN PPPPGGSNLE KWYYSTINHT
360 370 380 390 400
SAPGGTPPLA SLGSEGYKLL AGLLEYDPSK RLTAAQALQS PFFSTGDRVS
410 420 430 440 450
ANCFEGCKNE YPCRRVSQDD NDIRTSSLPG TKRSGLPDDS LIRPAKRQKE
Length:450
Mass (Da):50,892
Last modified:August 30, 2005 - v1
Checksum:i0DDBF7B8C07F2334
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ091181 Genomic DNA. Translation: AAY99646.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ091181 Genomic DNA. Translation: AAY99646.1.

3D structure databases

ProteinModelPortaliQ4FCZ5.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Fck1, a C-type cyclin-dependent kinase, interacts with Fcc1 to regulate development and secondary metabolism in Fusarium verticillioides."
    Bluhm B.H., Woloshuk C.P.
    Fungal Genet. Biol. 43:146-154(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH SSN8.

Entry informationi

Entry nameiSSN3_GIBMO
AccessioniPrimary (citable) accession number: Q4FCZ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 30, 2005
Last modified: January 7, 2015
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.