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Protein

L-amino-acid oxidase

Gene
N/A
Organism
Daboia siamensis (Eastern Russel's viper) (Daboia russelii siamensis)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes an oxidative deamination of predominantly hydrophobic and aromatic L-amino acids (highly active against L-Leu followed by L-Phe and L-Ile and not active against L-Pro, L-Asn, L-Gly, L-Ser and L-Cys), thus producing hydrogen peroxide that may contribute to the diverse toxic effects of this enzyme. Exhibits diverse biological activities such as antibacterial activity (Minimal inhibitory concentrations (MIC) are 9.0 µg/ml against S.aureus, 144.0 µg/ml against P.aeruginosa and 288.0 µg/ml against E.coli) and inhibition of ADP- and TMVA-induced platelet aggregation. Effects of snake L-amino oxidases on platelets are controversial, since they either induce aggregation or inhibit agonist-induced aggregation. These different effects are probably due to different experimental conditions. Unlike other snake venom L-amino acid oxidases, does not induce hemorrhage. This protein may also induce hemolysis, edema, apoptosis and have antiparasitic activities.1 Publication

Catalytic activityi

An L-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.1 Publication

Cofactori

FADBy similarity

pH dependencei

Optimum pH is 8.8.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei144 – 1441SubstrateBy similarity
Binding sitei182 – 1821FAD; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei293 – 2931SubstrateBy similarity
Binding sitei378 – 3781FADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi385 – 3906FADBy similarity
Nucleotide bindingi385 – 3862SubstrateBy similarity

GO - Molecular functioni

  1. L-amino-acid oxidase activity Source: UniProtKB
  2. protein homodimerization activity Source: UniProtKB

GO - Biological processi

  1. apoptotic process Source: UniProtKB-KW
  2. defense response to bacterium Source: UniProtKB
  3. hemolysis in other organism Source: UniProtKB-KW
  4. negative regulation of platelet aggregation in other organism Source: UniProtKB
  5. oxidation-reduction process Source: UniProtKB
  6. pathogenesis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Hemostasis impairing toxin, Oxidoreductase, Platelet aggregation inhibiting toxin, Toxin

Keywords - Biological processi

Apoptosis, Cytolysis, Hemolysis

Keywords - Ligandi

FAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
L-amino-acid oxidase (EC:1.4.3.2)
Short name:
DRS-LAAO
Short name:
LAAO
Short name:
LAO
OrganismiDaboia siamensis (Eastern Russel's viper) (Daboia russelii siamensis)
Taxonomic identifieri343250 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeViperinaeDaboia

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 407›407L-amino-acid oxidasePRO_0000315372Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi10 ↔ 94By similarity
Glycosylationi93 – 931N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi252 ↔ 333By similarity
Glycosylationi282 – 2821N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Expressed by the venom gland.1 Publication

Interactioni

Subunit structurei

Homodimer; non-covalently linked.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ4F867.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG005729.

Family and domain databases

InterProiIPR002937. Amino_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragments.

Q4F867-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
ADDKNPLEEC FREDDHRIVR EYIRKFGLKL NEFVQETENG WYFIKNIRKR
60 70 80 90 100
VGEVKKDPGL LKYPVKPSEA GKSAGQLYQE SLGKAVEELK RTNCSYILNK
110 120 130 140 150
YDTYSTKEYL IKEGNLSPGA VDMIGDLLNE DSGYYVSFIE SLKHDDIFAY
160 170 180 190 200
EKRFDEIVGG MDQLPTSMYR AIEESVRFKA RVIKIQQNAE KVTVTYQTTQ
210 220 230 240 250
KNLLLETVDY VIVCTTSRAA RRITFKPPLP PKKAHALRSV HYRSGTKIFL
260 270 280 290 300
TCTKKFWEDD GIQGGKSTTD LPSRFIYYPN HNFTTGVGVI IAYGIGDDAN
310 320 330 340 350
FFQALNLNEC ADIVFNDLSS IHQLPKKDLQ TFCYPSIIQK WSLDKYAMGA
360 370 380 390 400
ITTFTPYQFQ HFSEALTAPV GRIFFAGEYT ANAHGWIDST IKSGLTAARD

VNRASEL
Length:407
Mass (Da):46,372
Last modified:January 19, 2010 - v2
Checksum:i8B7B22226EF561B9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11
Non-adjacent residuesi15 – 162Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ104365 mRNA. Translation: AAZ08620.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ104365 mRNA. Translation: AAZ08620.1.

3D structure databases

ProteinModelPortaliQ4F867.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG005729.

Family and domain databases

InterProiIPR002937. Amino_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Purification and characterization of a new L-amino acid oxidase from Daboia russellii siamensis venom."
    Zhong S.-R., Jin Y., Wu J.-B., Jia Y.-H., Xu G.-L., Wang G.-C., Xiong Y.-L., Lu Q.-M.
    Toxicon 54:763-771(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-15, NUCLEOTIDE SEQUENCE [MRNA] OF 16-407, FUNCTION, CATALYTIC ACTIVITY, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GLYCOSYLATION, BIOPHYSICOCHEMICAL PROPERTIES.
    Tissue: Venom and Venom gland.

Entry informationi

Entry nameiOXLA_DABSI
AccessioniPrimary (citable) accession number: Q4F867
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 19, 2010
Last modified: November 26, 2014
This is version 39 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.