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Q4F867

- OXLA_DABSI

UniProt

Q4F867 - OXLA_DABSI

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Protein
L-amino-acid oxidase
Gene
N/A
Organism
Daboia siamensis (Eastern Russel's viper) (Daboia russelii siamensis)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes an oxidative deamination of predominantly hydrophobic and aromatic L-amino acids (highly active against L-Leu followed by L-Phe and L-Ile and not active against L-Pro, L-Asn, L-Gly, L-Ser and L-Cys), thus producing hydrogen peroxide that may contribute to the diverse toxic effects of this enzyme. Exhibits diverse biological activities such as antibacterial activity (Minimal inhibitory concentrations (MIC) are 9.0 µg/ml against S.aureus, 144.0 µg/ml against P.aeruginosa and 288.0 µg/ml against E.coli) and inhibition of ADP- and TMVA-induced platelet aggregation. Effects of snake L-amino oxidases on platelets are controversial, since they either induce aggregation or inhibit agonist-induced aggregation. These different effects are probably due to different experimental conditions. Unlike other snake venom L-amino acid oxidases, does not induce hemorrhage. This protein may also induce hemolysis, edema, apoptosis and have antiparasitic activities.1 Publication

Catalytic activityi

An L-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.1 Publication

Cofactori

FAD By similarity.

pH dependencei

Optimum pH is 8.8.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei144 – 1441Substrate By similarity
Binding sitei182 – 1821FAD; via amide nitrogen and carbonyl oxygen By similarity
Binding sitei293 – 2931Substrate By similarity
Binding sitei378 – 3781FAD By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi385 – 3906FAD By similarity
Nucleotide bindingi385 – 3862Substrate By similarity

GO - Molecular functioni

  1. L-amino-acid oxidase activity Source: UniProtKB
  2. protein homodimerization activity Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. apoptotic process Source: UniProtKB-KW
  2. defense response to bacterium Source: UniProtKB
  3. hemolysis in other organism Source: UniProtKB-KW
  4. oxidation-reduction process Source: UniProtKB
  5. pathogenesis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Hemostasis impairing toxin, Oxidoreductase, Platelet aggregation inhibiting toxin, Toxin

Keywords - Biological processi

Apoptosis, Cytolysis, Hemolysis

Keywords - Ligandi

FAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
L-amino-acid oxidase (EC:1.4.3.2)
Short name:
DRS-LAAO
Short name:
LAAO
Short name:
LAO
OrganismiDaboia siamensis (Eastern Russel's viper) (Daboia russelii siamensis)
Taxonomic identifieri343250 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeViperinaeDaboia

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 407›407L-amino-acid oxidase
PRO_0000315372Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi10 ↔ 94 By similarity
Glycosylationi93 – 931N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi252 ↔ 333 By similarity
Glycosylationi282 – 2821N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

N-glycosylated Inferred.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Expressed by the venom gland.1 Publication

Interactioni

Subunit structurei

Homodimer; non-covalently linked.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ4F867.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG005729.

Family and domain databases

InterProiIPR002937. Amino_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragments.

Q4F867-1 [UniParc]FASTAAdd to Basket

« Hide

ADDKNPLEEC FREDDHRIVR EYIRKFGLKL NEFVQETENG WYFIKNIRKR    50
VGEVKKDPGL LKYPVKPSEA GKSAGQLYQE SLGKAVEELK RTNCSYILNK 100
YDTYSTKEYL IKEGNLSPGA VDMIGDLLNE DSGYYVSFIE SLKHDDIFAY 150
EKRFDEIVGG MDQLPTSMYR AIEESVRFKA RVIKIQQNAE KVTVTYQTTQ 200
KNLLLETVDY VIVCTTSRAA RRITFKPPLP PKKAHALRSV HYRSGTKIFL 250
TCTKKFWEDD GIQGGKSTTD LPSRFIYYPN HNFTTGVGVI IAYGIGDDAN 300
FFQALNLNEC ADIVFNDLSS IHQLPKKDLQ TFCYPSIIQK WSLDKYAMGA 350
ITTFTPYQFQ HFSEALTAPV GRIFFAGEYT ANAHGWIDST IKSGLTAARD 400
VNRASEL 407
Length:407
Mass (Da):46,372
Last modified:January 19, 2010 - v2
Checksum:i8B7B22226EF561B9
GO

Non-adjacent residues

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-adjacent residuesi15 – 162

Non-terminal residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ104365 mRNA. Translation: AAZ08620.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ104365 mRNA. Translation: AAZ08620.1 .

3D structure databases

ProteinModelPortali Q4F867.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG005729.

Family and domain databases

InterProi IPR002937. Amino_oxidase.
[Graphical view ]
Pfami PF01593. Amino_oxidase. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Purification and characterization of a new L-amino acid oxidase from Daboia russellii siamensis venom."
    Zhong S.-R., Jin Y., Wu J.-B., Jia Y.-H., Xu G.-L., Wang G.-C., Xiong Y.-L., Lu Q.-M.
    Toxicon 54:763-771(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-15, NUCLEOTIDE SEQUENCE [MRNA] OF 16-407, FUNCTION, CATALYTIC ACTIVITY, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GLYCOSYLATION, BIOPHYSICOCHEMICAL PROPERTIES.
    Tissue: Venom and Venom gland.

Entry informationi

Entry nameiOXLA_DABSI
AccessioniPrimary (citable) accession number: Q4F867
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 19, 2010
Last modified: April 16, 2014
This is version 36 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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