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Protein

Catalase-peroxidase 2

Gene

katG2

Organism
Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315))
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity. Shows peroxidase specificity towards odianisidine, ABTS and pyrogallol, but methoxyphenol and 2-chloronaphthol are not peroxidized.UniRule annotation1 Publication

Catalytic activityi

Donor + H2O2 = oxidized donor + 2 H2O.UniRule annotation
2 H2O2 = O2 + 2 H2O.UniRule annotation

Cofactori

heme bUniRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per dimer.UniRule annotation

pH dependencei

Optimum pH is 5.5 for the peroxidase reaction and 6.0 for the catalase reaction. Active from pH 5.5 to 8.5.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei88 – 881Transition state stabilizerUniRule annotation
Active sitei92 – 921Proton acceptorUniRule annotation
Metal bindingi268 – 2681Iron (heme axial ligand)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciBCEN216591:GJI4-5734-MONOMER.
BRENDAi1.11.1.6. 8982.

Protein family/group databases

PeroxiBasei2309. BcenCP01_J2315.

Names & Taxonomyi

Protein namesi
Recommended name:
Catalase-peroxidase 2UniRule annotation (EC:1.11.1.21UniRule annotation)
Short name:
CP 2UniRule annotation
Alternative name(s):
Peroxidase/catalase 2UniRule annotation
Gene namesi
Name:katG2UniRule annotation
Synonyms:katA
Ordered Locus Names:BceJ2315_55450
ORF Names:BCAM2107
OrganismiBurkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315))
Taxonomic identifieri216591 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
Proteomesi
  • UP000001035 Componenti: Chromosome 2

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 736736Catalase-peroxidase 2PRO_0000354740Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki91 ↔ 227Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-253)UniRule annotation
Cross-linki227 ↔ 253Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-91)UniRule annotation

Post-translational modificationi

The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme.UniRule annotation

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi216591.BCAM2107.

Structurei

3D structure databases

ProteinModelPortaliQ4F6N6.
SMRiQ4F6N6. Positions 16-736.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Peroxidase/catalase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0376. LUCA.
HOGENOMiHOG000218110.
KOiK03782.
OMAiNEMLAND.
OrthoDBiEOG6RRKKM.

Family and domain databases

HAMAPiMF_01961. Catal_peroxid.
InterProiIPR000763. Catalase_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 2 hits.
[Graphical view]
PRINTSiPR00460. BPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 2 hits.
TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4F6N6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNEGQCPFN HANGGGTTNR DWWPNELRLD LLSQHSSKTD PLDPGFNYAE
60 70 80 90 100
AFNSLDLDAL RKDLAALMTD SQDWWPADFG HYGPLFVRMA WHSAGTYRMG
110 120 130 140 150
DGRGGAGRGQ QRFAPLNSWP DNVSLDKARR LLWPIKQKYG QKISWADLLI
160 170 180 190 200
LTGDVALTTM GFKTFGYAGG REDTWEPDRD VYWGSETTWL GGDLRYDKGG
210 220 230 240 250
ACESQHGGNA GRNLENPLAA VQMGLIYVNP EGPDGNPDPV AAAYDIREVF
260 270 280 290 300
GRMAMNDEET VALIAGGHAF GKTHGAGPAD NVGLEPEAAG LEQQGLGWKN
310 320 330 340 350
SFGTGKGADT ITSGLEVTWS DTPTQWGMGF FKNLFGYEWE LTKSPAGAHQ
360 370 380 390 400
WVAKNAEPTI PHAHDPSKKL LPTMLTTDLS LRFDPVYEKI SRHFMDNPDV
410 420 430 440 450
FADAFARAWF KLTHRDMGPR ARYLGPDVPT EELIWQDPIP AVDHVLVDDT
460 470 480 490 500
DVAPLKETIL ASGLSVAELV STAWASASTF RGSDKRGGAN GARIRLAPQK
510 520 530 540 550
DWAVNEPARL AKVLKVLERI QGEFNSTQPG GKKISLADLI VLAGGAGIEQ
560 570 580 590 600
AAKRAGHDVV VPFAPGRMDA SQEQTDAHSF AVLEPVADGF RNFVKGKFAV
610 620 630 640 650
PAEALLIDKA QLLTLTAPQM TALVGGLRVL NVQTGDEKHG VFTDQPETLT
660 670 680 690 700
VDFFRNLLDM ATEWKPIAGE DTYEGRDRRT GELKWTGTRV DLVFGSNAVL
710 720 730
RALSEVYASA DGEAKFIRDF VAAWVKVMNL DRFDLA
Length:736
Mass (Da):80,487
Last modified:August 30, 2005 - v1
Checksum:i48CC314FEA9C30A3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ112341 Genomic DNA. Translation: AAZ14051.1.
AM747721 Genomic DNA. Translation: CAR55964.1.
RefSeqiWP_006490370.1. NC_011001.1.

Genome annotation databases

EnsemblBacteriaiCAR55964; CAR55964; BCAM2107.
GeneIDi6929708.
KEGGibcj:BCAM2107.
PATRICi19083523. VBIBurCen118154_5878.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ112341 Genomic DNA. Translation: AAZ14051.1.
AM747721 Genomic DNA. Translation: CAR55964.1.
RefSeqiWP_006490370.1. NC_011001.1.

3D structure databases

ProteinModelPortaliQ4F6N6.
SMRiQ4F6N6. Positions 16-736.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi216591.BCAM2107.

Protein family/group databases

PeroxiBasei2309. BcenCP01_J2315.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAR55964; CAR55964; BCAM2107.
GeneIDi6929708.
KEGGibcj:BCAM2107.
PATRICi19083523. VBIBurCen118154_5878.

Phylogenomic databases

eggNOGiCOG0376. LUCA.
HOGENOMiHOG000218110.
KOiK03782.
OMAiNEMLAND.
OrthoDBiEOG6RRKKM.

Enzyme and pathway databases

BioCyciBCEN216591:GJI4-5734-MONOMER.
BRENDAi1.11.1.6. 8982.

Family and domain databases

HAMAPiMF_01961. Catal_peroxid.
InterProiIPR000763. Catalase_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 2 hits.
[Graphical view]
PRINTSiPR00460. BPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 2 hits.
TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a bifunctional catalase-peroxidase of Burkholderia cenocepacia."
    Charalabous P., Risk J.M., Jenkins R., Birss A.J., Hart C.A., Smalley J.W.
    FEMS Immunol. Med. Microbiol. 50:37-44(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610.

Entry informationi

Entry nameiKATG2_BURCJ
AccessioniPrimary (citable) accession number: Q4F6N6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: August 30, 2005
Last modified: December 9, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.