Reviewed, UniProtKB/Swiss-Prot Q4DLX9 (JBP1B_TRYCR)

Names and origin

Protein names

Recommended name:
    Thymine dioxygenase JBP1-B
    EC=1.14.11.6
Alternative name(s):
    J-binding protein 1B

Gene names

Name:	JBP1B
ORF Names:	Tc00.1047053506753.120

Organism

Trypanosoma cruzi

Taxonomic identifier

5693 [NCBI]

Taxonomic lineage

Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Trypanosoma › Schizotrypanum

Protein attributes

Sequence length	831 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Dioxygenase that catalyzes the first step of DNA base J (beta-d-glucosyl-HOMedU) biosynthesis by converting thymine to 5-hydroxymethyluracil (HOMedU). DNA base J is a hypermodified thymidine residue found in the genome of kinetoplastid parasites, which is localized primarily to repetitive DNA, namely the telomeres, and is implicated in the regulation of antigenic variation. Specifically binds DNA base J. Involved in propagation and maintenance of DNA base J synthesis initiated by JBP2 by specifically binding already synthesized DNA base J and propagating J synthesis By similarity.
Catalytic activity	Thymine + 2-oxoglutarate + O2 = 5-hydroxymethyluracil + succinate + CO2.
Cofactor	Binds 1 Fe2+ ion per subunit By similarity.
Subcellular location	Nucleus By similarity.
Domain	The Myb-like region is required for the specific binding to DNA base J By similarity.
Sequence similarities	Belongs to the TET family. JBP1 subfamily.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	DNA-binding Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW thymine dioxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 831	831	Thymine dioxygenase JBP1-B		PRO_0000377557
Regions
	Region	271 – 294	24	Myb-like region
Sites
	Metal binding	207	1	Iron; catalytic By similarity
	Metal binding	209	1	Iron; catalytic By similarity
	Metal binding	257	1	Iron; catalytic By similarity
	Binding site	273	1	2-oxoglutarate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4DLX9-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 8AC5FC67B4CE1595	FASTA	831	93,540
	10 20 30 40 50 60 MKQKRGKQDV KMVESAPPQL LPKKGRLEIS ELAPQQRTIR TAEEIEMAYN EAVRKHPFYD 70 80 90 100 110 120 NADHTIDFHD ATVFRDARGV VGGVLLPGAL PAFAATMAAD VLRPAAVRTS LRSNMFGGFA 130 140 150 160 170 180 PLSGIAGYFD YRGSPVELKC RKTSFTYENV HSWPNVFPMI DYVSAIYKAV FPERWAAQDA 190 200 210 220 230 240 AVPDIVRIHG SPFSTLTVNQ QFRTASHTDA GDFDMGYGLL AVLEGKFEGL SLALDDFGVC 250 260 270 280 290 300 FRMQPRDVLI FNTHFFHSNT EPELNHPKDD WSRLTCVCYY RAALGEPACV AEYERRLARA 310 320 330 340 350 360 KEIGASPPPA VDAILQKDNG NNFNKPAPTF TYSLTPFGGA ASICSLHCCT AKLLRLHELL 370 380 390 400 410 420 LENPTLEVIL FGESLRTDDG LPRREKEQLI SVHLPVVVKM SPSGGFSELG GALKAAEEKQ 430 440 450 460 470 480 YFFEEKYLAD ELGPDLMSMW TQSRAHWLRL VKEDWERLCR RDPERTKFTW NNSSAMNAAF 490 500 510 520 530 540 FDLCEVAKQM MIGLLNKETP SSAENHSFWI LFAAHLNYAC TTENGMPRDA VGMHKLNVKL 550 560 570 580 590 600 KDFHFGGTRY LKDMPPEEQE RRLERKKRIE EARRRGNAAR ETHTDNWLLN DTFDYQQEDR 610 620 630 640 650 660 KVEFEENGWM TPEAYVKHLG LKPCGDVTAA ASPTEPIHVL VVLPRPAAAA PKDVKRDVPL 670 680 690 700 710 720 ATSEESIRLL MNPAAQRVLT GKARNVTLPS PLSFGGVKIT VLFDGDDIDC IHPDFVVLQH 730 740 750 760 770 780 LLAAIEEDEA AKARVKYWAH VARYCVFVVE TDVRDRRHFL LREEVRVAYE DVAEDCFRSL 790 800 810 820 830 HAAAYSTKCN RLRTTPSLIA LSNSKNIGLR FKFRGSPLNT IALIVVGERL D

References

[1]

"The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease." El-Sayed N.M.A., Myler P.J., Bartholomeu D.C., Nilsson D., Aggarwal G., Tran A.-N., Ghedin E., Worthey E.A., Delcher A.L., Blandin G., Westenberger S.J., Caler E., Cerqueira G.C., Branche C., Haas B., Anupama A., Arner E., Aslund L. , Attipoe P., Bontempi E., Bringaud F., Burton P., Cadag E., Campbell D.A., Carrington M., Crabtree J., Darban H., da Silveira J.F., de Jong P., Edwards K., Englund P.T., Fazelina G., Feldblyum T., Ferella M., Frasch A.C., Gull K., Horn D., Hou L., Huang Y., Kindlund E., Klingbeil M., Kluge S., Koo H., Lacerda D., Levin M.J., Lorenzi H., Louie T., Machado C.R., McCulloch R., McKenna A., Mizuno Y., Mottram J.C., Nelson S., Ochaya S., Osoegawa K., Pai G., Parsons M., Pentony M., Pettersson U., Pop M., Ramirez J.L., Rinta J., Robertson L., Salzberg S.L., Sanchez D.O., Seyler A., Sharma R., Shetty J., Simpson A.J., Sisk E., Tammi M.T., Tarleton R., Teixeira S., Van Aken S., Vogt C., Ward P.N., Wickstead B., Wortman J., White O., Fraser C.M., Stuart K.D., Andersson B. Science 309:409-415(2005) [PubMed: 16020725] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CL Brener.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAHK01000343 Genomic DNA. Translation: EAN93526.1.
RefSeq	XP_815377.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3547091.
KEGG	tcr:506753.120.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

JBP1B_TRYCR

Accession

Primary (citable) accession number: Q4DLX9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2009
Last sequence update:	September 13, 2005
Last modified:	September 22, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)