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Q4DLX9

- JBP1B_TRYCC

UniProt

Q4DLX9 - JBP1B_TRYCC

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Protein

Thymine dioxygenase JBP1-B

Gene
JBP1B, Tc00.1047053506753.120
Organism
Trypanosoma cruzi (strain CL Brener)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Dioxygenase that catalyzes the first step of DNA base J (beta-d-glucosyl-HOMedU) biosynthesis by converting thymine to 5-hydroxymethyluracil (HOMedU). DNA base J is a hypermodified thymidine residue found in the genome of kinetoplastid parasites, which is localized primarily to repetitive DNA, namely the telomeres, and is implicated in the regulation of antigenic variation. Also specifically binds to base J-containing DNA (J-DNA). Involved in propagation and maintenance of DNA base J synthesis initiated by JBP2 by specifically binding already synthesized DNA base J and propagating J synthesis. Thymine dioxygenase activity and J-DNA-binding are independent functions By similarity.

Catalytic activityi

Thymine + 2-oxoglutarate + O2 = 5-hydroxymethyluracil + succinate + CO2.

Cofactori

Binds 1 Fe2+ ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi207 – 2071Iron; catalytic By similarity
Metal bindingi209 – 2091Iron; catalytic By similarity
Metal bindingi257 – 2571Iron; catalytic By similarity
Binding sitei273 – 27312-oxoglutarate By similarity
Sitei542 – 5421Involved in J base recognition, conferring specificity towards J-DNA By similarity

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. thymine dioxygenase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Ligandi

DNA-binding, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Thymine dioxygenase JBP1-B (EC:1.14.11.6)
Alternative name(s):
J-binding protein 1B
Thymidine hydroxylase JBP1-B
Gene namesi
Name:JBP1B
ORF Names:Tc00.1047053506753.120
OrganismiTrypanosoma cruzi (strain CL Brener)
Taxonomic identifieri353153 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosomaSchizotrypanum
ProteomesiUP000002296: Unassembled WGS sequence

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 831831Thymine dioxygenase JBP1-BPRO_0000377557Add
BLAST

Interactioni

Subunit structurei

Monomer. Binds to DNA as a monomer By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ4DLX9.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni80 – 282203Thymine dioxygenase By similarityAdd
BLAST
Regioni409 – 578170DNA-binding JBP1 domain By similarityAdd
BLAST

Domaini

The DNA-binding JBP1 domain (DB-JBP1) is necessary and sufficient for binding to J-DNA By similarity.

Sequence similaritiesi

Belongs to the TET family. JBP1 subfamily.

Family and domain databases

InterProiIPR024779. 2OGFeDO_nucleic_acid_mod.
[Graphical view]
PfamiPF12851. Tet_JBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4DLX9-1 [UniParc]FASTAAdd to Basket

« Hide

MKQKRGKQDV KMVESAPPQL LPKKGRLEIS ELAPQQRTIR TAEEIEMAYN    50
EAVRKHPFYD NADHTIDFHD ATVFRDARGV VGGVLLPGAL PAFAATMAAD 100
VLRPAAVRTS LRSNMFGGFA PLSGIAGYFD YRGSPVELKC RKTSFTYENV 150
HSWPNVFPMI DYVSAIYKAV FPERWAAQDA AVPDIVRIHG SPFSTLTVNQ 200
QFRTASHTDA GDFDMGYGLL AVLEGKFEGL SLALDDFGVC FRMQPRDVLI 250
FNTHFFHSNT EPELNHPKDD WSRLTCVCYY RAALGEPACV AEYERRLARA 300
KEIGASPPPA VDAILQKDNG NNFNKPAPTF TYSLTPFGGA ASICSLHCCT 350
AKLLRLHELL LENPTLEVIL FGESLRTDDG LPRREKEQLI SVHLPVVVKM 400
SPSGGFSELG GALKAAEEKQ YFFEEKYLAD ELGPDLMSMW TQSRAHWLRL 450
VKEDWERLCR RDPERTKFTW NNSSAMNAAF FDLCEVAKQM MIGLLNKETP 500
SSAENHSFWI LFAAHLNYAC TTENGMPRDA VGMHKLNVKL KDFHFGGTRY 550
LKDMPPEEQE RRLERKKRIE EARRRGNAAR ETHTDNWLLN DTFDYQQEDR 600
KVEFEENGWM TPEAYVKHLG LKPCGDVTAA ASPTEPIHVL VVLPRPAAAA 650
PKDVKRDVPL ATSEESIRLL MNPAAQRVLT GKARNVTLPS PLSFGGVKIT 700
VLFDGDDIDC IHPDFVVLQH LLAAIEEDEA AKARVKYWAH VARYCVFVVE 750
TDVRDRRHFL LREEVRVAYE DVAEDCFRSL HAAAYSTKCN RLRTTPSLIA 800
LSNSKNIGLR FKFRGSPLNT IALIVVGERL D 831
Length:831
Mass (Da):93,540
Last modified:September 13, 2005 - v1
Checksum:i8AC5FC67B4CE1595
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAHK01000343 Genomic DNA. Translation: EAN93526.1.
RefSeqiXP_815377.1. XM_810284.1.

Genome annotation databases

GeneIDi3547091.
KEGGitcr:506753.120.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAHK01000343 Genomic DNA. Translation: EAN93526.1 .
RefSeqi XP_815377.1. XM_810284.1.

3D structure databases

ProteinModelPortali Q4DLX9.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3547091.
KEGGi tcr:506753.120.

Family and domain databases

InterProi IPR024779. 2OGFeDO_nucleic_acid_mod.
[Graphical view ]
Pfami PF12851. Tet_JBP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease."
    El-Sayed N.M.A., Myler P.J., Bartholomeu D.C., Nilsson D., Aggarwal G., Tran A.-N., Ghedin E., Worthey E.A., Delcher A.L., Blandin G., Westenberger S.J., Caler E., Cerqueira G.C., Branche C., Haas B., Anupama A., Arner E., Aslund L.
    , Attipoe P., Bontempi E., Bringaud F., Burton P., Cadag E., Campbell D.A., Carrington M., Crabtree J., Darban H., da Silveira J.F., de Jong P., Edwards K., Englund P.T., Fazelina G., Feldblyum T., Ferella M., Frasch A.C., Gull K., Horn D., Hou L., Huang Y., Kindlund E., Klingbeil M., Kluge S., Koo H., Lacerda D., Levin M.J., Lorenzi H., Louie T., Machado C.R., McCulloch R., McKenna A., Mizuno Y., Mottram J.C., Nelson S., Ochaya S., Osoegawa K., Pai G., Parsons M., Pentony M., Pettersson U., Pop M., Ramirez J.L., Rinta J., Robertson L., Salzberg S.L., Sanchez D.O., Seyler A., Sharma R., Shetty J., Simpson A.J., Sisk E., Tammi M.T., Tarleton R., Teixeira S., Van Aken S., Vogt C., Ward P.N., Wickstead B., Wortman J., White O., Fraser C.M., Stuart K.D., Andersson B.
    Science 309:409-415(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CL Brener.

Entry informationi

Entry nameiJBP1B_TRYCC
AccessioniPrimary (citable) accession number: Q4DLX9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: September 13, 2005
Last modified: May 14, 2014
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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