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Q4DLX9

- JBP1B_TRYCC

UniProt

Q4DLX9 - JBP1B_TRYCC

Protein

Thymine dioxygenase JBP1-B

Gene

JBP1B

Organism
Trypanosoma cruzi (strain CL Brener)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 26 (01 Oct 2014)
      Sequence version 1 (13 Sep 2005)
      Previous versions | rss
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    Functioni

    Dioxygenase that catalyzes the first step of DNA base J (beta-d-glucosyl-HOMedU) biosynthesis by converting thymine to 5-hydroxymethyluracil (HOMedU). DNA base J is a hypermodified thymidine residue found in the genome of kinetoplastid parasites, which is localized primarily to repetitive DNA, namely the telomeres, and is implicated in the regulation of antigenic variation. Also specifically binds to base J-containing DNA (J-DNA). Involved in propagation and maintenance of DNA base J synthesis initiated by JBP2 by specifically binding already synthesized DNA base J and propagating J synthesis. Thymine dioxygenase activity and J-DNA-binding are independent functions By similarity.By similarity

    Catalytic activityi

    Thymine + 2-oxoglutarate + O2 = 5-hydroxymethyluracil + succinate + CO2.

    Cofactori

    Binds 1 Fe2+ ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi207 – 2071Iron; catalyticBy similarity
    Metal bindingi209 – 2091Iron; catalyticBy similarity
    Metal bindingi257 – 2571Iron; catalyticBy similarity
    Binding sitei273 – 27312-oxoglutarateBy similarity
    Sitei542 – 5421Involved in J base recognition, conferring specificity towards J-DNABy similarity

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. thymine dioxygenase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Dioxygenase, Oxidoreductase

    Keywords - Ligandi

    DNA-binding, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymine dioxygenase JBP1-B (EC:1.14.11.6)
    Alternative name(s):
    J-binding protein 1B
    Thymidine hydroxylase JBP1-B
    Gene namesi
    Name:JBP1B
    ORF Names:Tc00.1047053506753.120
    OrganismiTrypanosoma cruzi (strain CL Brener)
    Taxonomic identifieri353153 [NCBI]
    Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosomaSchizotrypanum
    ProteomesiUP000002296: Unassembled WGS sequence

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 831831Thymine dioxygenase JBP1-BPRO_0000377557Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer. Binds to DNA as a monomer By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ4DLX9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni80 – 282203Thymine dioxygenaseBy similarityAdd
    BLAST
    Regioni409 – 578170DNA-binding JBP1 domainBy similarityAdd
    BLAST

    Domaini

    The DNA-binding JBP1 domain (DB-JBP1) is necessary and sufficient for binding to J-DNA.By similarity

    Sequence similaritiesi

    Belongs to the TET family. JBP1 subfamily.Curated

    Family and domain databases

    InterProiIPR024779. 2OGFeDO_nucleic_acid_mod.
    [Graphical view]
    PfamiPF12851. Tet_JBP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q4DLX9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKQKRGKQDV KMVESAPPQL LPKKGRLEIS ELAPQQRTIR TAEEIEMAYN    50
    EAVRKHPFYD NADHTIDFHD ATVFRDARGV VGGVLLPGAL PAFAATMAAD 100
    VLRPAAVRTS LRSNMFGGFA PLSGIAGYFD YRGSPVELKC RKTSFTYENV 150
    HSWPNVFPMI DYVSAIYKAV FPERWAAQDA AVPDIVRIHG SPFSTLTVNQ 200
    QFRTASHTDA GDFDMGYGLL AVLEGKFEGL SLALDDFGVC FRMQPRDVLI 250
    FNTHFFHSNT EPELNHPKDD WSRLTCVCYY RAALGEPACV AEYERRLARA 300
    KEIGASPPPA VDAILQKDNG NNFNKPAPTF TYSLTPFGGA ASICSLHCCT 350
    AKLLRLHELL LENPTLEVIL FGESLRTDDG LPRREKEQLI SVHLPVVVKM 400
    SPSGGFSELG GALKAAEEKQ YFFEEKYLAD ELGPDLMSMW TQSRAHWLRL 450
    VKEDWERLCR RDPERTKFTW NNSSAMNAAF FDLCEVAKQM MIGLLNKETP 500
    SSAENHSFWI LFAAHLNYAC TTENGMPRDA VGMHKLNVKL KDFHFGGTRY 550
    LKDMPPEEQE RRLERKKRIE EARRRGNAAR ETHTDNWLLN DTFDYQQEDR 600
    KVEFEENGWM TPEAYVKHLG LKPCGDVTAA ASPTEPIHVL VVLPRPAAAA 650
    PKDVKRDVPL ATSEESIRLL MNPAAQRVLT GKARNVTLPS PLSFGGVKIT 700
    VLFDGDDIDC IHPDFVVLQH LLAAIEEDEA AKARVKYWAH VARYCVFVVE 750
    TDVRDRRHFL LREEVRVAYE DVAEDCFRSL HAAAYSTKCN RLRTTPSLIA 800
    LSNSKNIGLR FKFRGSPLNT IALIVVGERL D 831
    Length:831
    Mass (Da):93,540
    Last modified:September 13, 2005 - v1
    Checksum:i8AC5FC67B4CE1595
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAHK01000343 Genomic DNA. Translation: EAN93526.1.
    RefSeqiXP_815377.1. XM_810284.1.

    Genome annotation databases

    GeneIDi3547091.
    KEGGitcr:506753.120.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAHK01000343 Genomic DNA. Translation: EAN93526.1 .
    RefSeqi XP_815377.1. XM_810284.1.

    3D structure databases

    ProteinModelPortali Q4DLX9.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 3547091.
    KEGGi tcr:506753.120.

    Family and domain databases

    InterProi IPR024779. 2OGFeDO_nucleic_acid_mod.
    [Graphical view ]
    Pfami PF12851. Tet_JBP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease."
      El-Sayed N.M.A., Myler P.J., Bartholomeu D.C., Nilsson D., Aggarwal G., Tran A.-N., Ghedin E., Worthey E.A., Delcher A.L., Blandin G., Westenberger S.J., Caler E., Cerqueira G.C., Branche C., Haas B., Anupama A., Arner E., Aslund L.
      , Attipoe P., Bontempi E., Bringaud F., Burton P., Cadag E., Campbell D.A., Carrington M., Crabtree J., Darban H., da Silveira J.F., de Jong P., Edwards K., Englund P.T., Fazelina G., Feldblyum T., Ferella M., Frasch A.C., Gull K., Horn D., Hou L., Huang Y., Kindlund E., Klingbeil M., Kluge S., Koo H., Lacerda D., Levin M.J., Lorenzi H., Louie T., Machado C.R., McCulloch R., McKenna A., Mizuno Y., Mottram J.C., Nelson S., Ochaya S., Osoegawa K., Pai G., Parsons M., Pentony M., Pettersson U., Pop M., Ramirez J.L., Rinta J., Robertson L., Salzberg S.L., Sanchez D.O., Seyler A., Sharma R., Shetty J., Simpson A.J., Sisk E., Tammi M.T., Tarleton R., Teixeira S., Van Aken S., Vogt C., Ward P.N., Wickstead B., Wortman J., White O., Fraser C.M., Stuart K.D., Andersson B.
      Science 309:409-415(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CL Brener.

    Entry informationi

    Entry nameiJBP1B_TRYCC
    AccessioniPrimary (citable) accession number: Q4DLX9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 16, 2009
    Last sequence update: September 13, 2005
    Last modified: October 1, 2014
    This is version 26 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3