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Q4DLX9 (JBP1B_TRYCC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 25. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymine dioxygenase JBP1-B

EC=1.14.11.6
Alternative name(s):
J-binding protein 1B
Thymidine hydroxylase JBP1-B
Gene names
Name:JBP1B
ORF Names:Tc00.1047053506753.120
OrganismTrypanosoma cruzi (strain CL Brener) [Complete proteome]
Taxonomic identifier353153 [NCBI]
Taxonomic lineageEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosomaSchizotrypanum

Protein attributes

Sequence length831 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Dioxygenase that catalyzes the first step of DNA base J (beta-d-glucosyl-HOMedU) biosynthesis by converting thymine to 5-hydroxymethyluracil (HOMedU). DNA base J is a hypermodified thymidine residue found in the genome of kinetoplastid parasites, which is localized primarily to repetitive DNA, namely the telomeres, and is implicated in the regulation of antigenic variation. Also specifically binds to base J-containing DNA (J-DNA). Involved in propagation and maintenance of DNA base J synthesis initiated by JBP2 by specifically binding already synthesized DNA base J and propagating J synthesis. Thymine dioxygenase activity and J-DNA-binding are independent functions By similarity.

Catalytic activity

Thymine + 2-oxoglutarate + O2 = 5-hydroxymethyluracil + succinate + CO2.

Cofactor

Binds 1 Fe2+ ion per subunit By similarity.

Subunit structure

Monomer. Binds to DNA as a monomer By similarity.

Subcellular location

Nucleus By similarity.

Domain

The DNA-binding JBP1 domain (DB-JBP1) is necessary and sufficient for binding to J-DNA By similarity.

Sequence similarities

Belongs to the TET family. JBP1 subfamily.

Ontologies

Keywords
   Cellular componentNucleus
   LigandDNA-binding
Iron
Metal-binding
   Molecular functionDioxygenase
Oxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

thymine dioxygenase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 831831Thymine dioxygenase JBP1-B
PRO_0000377557

Regions

Region80 – 282203Thymine dioxygenase By similarity
Region409 – 578170DNA-binding JBP1 domain By similarity

Sites

Metal binding2071Iron; catalytic By similarity
Metal binding2091Iron; catalytic By similarity
Metal binding2571Iron; catalytic By similarity
Binding site27312-oxoglutarate By similarity
Site5421Involved in J base recognition, conferring specificity towards J-DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4DLX9 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: 8AC5FC67B4CE1595

FASTA83193,540
        10         20         30         40         50         60 
MKQKRGKQDV KMVESAPPQL LPKKGRLEIS ELAPQQRTIR TAEEIEMAYN EAVRKHPFYD 

        70         80         90        100        110        120 
NADHTIDFHD ATVFRDARGV VGGVLLPGAL PAFAATMAAD VLRPAAVRTS LRSNMFGGFA 

       130        140        150        160        170        180 
PLSGIAGYFD YRGSPVELKC RKTSFTYENV HSWPNVFPMI DYVSAIYKAV FPERWAAQDA 

       190        200        210        220        230        240 
AVPDIVRIHG SPFSTLTVNQ QFRTASHTDA GDFDMGYGLL AVLEGKFEGL SLALDDFGVC 

       250        260        270        280        290        300 
FRMQPRDVLI FNTHFFHSNT EPELNHPKDD WSRLTCVCYY RAALGEPACV AEYERRLARA 

       310        320        330        340        350        360 
KEIGASPPPA VDAILQKDNG NNFNKPAPTF TYSLTPFGGA ASICSLHCCT AKLLRLHELL 

       370        380        390        400        410        420 
LENPTLEVIL FGESLRTDDG LPRREKEQLI SVHLPVVVKM SPSGGFSELG GALKAAEEKQ 

       430        440        450        460        470        480 
YFFEEKYLAD ELGPDLMSMW TQSRAHWLRL VKEDWERLCR RDPERTKFTW NNSSAMNAAF 

       490        500        510        520        530        540 
FDLCEVAKQM MIGLLNKETP SSAENHSFWI LFAAHLNYAC TTENGMPRDA VGMHKLNVKL 

       550        560        570        580        590        600 
KDFHFGGTRY LKDMPPEEQE RRLERKKRIE EARRRGNAAR ETHTDNWLLN DTFDYQQEDR 

       610        620        630        640        650        660 
KVEFEENGWM TPEAYVKHLG LKPCGDVTAA ASPTEPIHVL VVLPRPAAAA PKDVKRDVPL 

       670        680        690        700        710        720 
ATSEESIRLL MNPAAQRVLT GKARNVTLPS PLSFGGVKIT VLFDGDDIDC IHPDFVVLQH 

       730        740        750        760        770        780 
LLAAIEEDEA AKARVKYWAH VARYCVFVVE TDVRDRRHFL LREEVRVAYE DVAEDCFRSL 

       790        800        810        820        830 
HAAAYSTKCN RLRTTPSLIA LSNSKNIGLR FKFRGSPLNT IALIVVGERL D 

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAHK01000343 Genomic DNA. Translation: EAN93526.1.
RefSeqXP_815377.1. XM_810284.1.

3D structure databases

ProteinModelPortalQ4DLX9.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3547091.
KEGGtcr:506753.120.

Family and domain databases

InterProIPR024779. 2OGFeDO_nucleic_acid_mod.
[Graphical view]
PfamPF12851. Tet_JBP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameJBP1B_TRYCC
AccessionPrimary (citable) accession number: Q4DLX9
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: September 13, 2005
Last modified: May 14, 2014
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families