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Protein

Lipoyl synthase 1, mitochondrial

Gene

Tc00.1047053511291.30

Organism
Trypanosoma cruzi (strain CL Brener)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Miscellaneous

This protein may be expected to contain an N-terminal transit peptide but none has been predicted.UniRule annotation

Catalytic activityi

Protein N6-(octanoyl)lysine + an [Fe-S] cluster scaffold protein carrying a [4Fe-4S]2+ cluster + 2 S-adenosyl-L-methionine + 2 oxidized [2Fe-2S] ferredoxin + 6 H+ = protein N6-(dihydrolipoyl)lysine + an [Fe-S] cluster scaffold protein + 2 sulfide + 4 Fe3+ + 2 L-methionine + 2 5'-deoxyadenosine + 2 reduced [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Lipoyl synthase 1, mitochondrial (Tc00.1047053511291.30), Lipoyl synthase 2, mitochondrial (Tc00.1047053506211.10)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi127Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi132Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi138Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi159Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi163Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi166Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase 1, mitochondrial (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthase 1UniRule annotation
Short name:
LS 1UniRule annotation
Short name:
Lip-syn 1UniRule annotation
Lipoic acid synthase 1UniRule annotation
Gene namesi
ORF Names:Tc00.1047053511291.30
OrganismiTrypanosoma cruzi (strain CL Brener)
Taxonomic identifieri353153 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosomaSchizotrypanum
Proteomesi
  • UP000002296 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Mitochondrion UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003982411 – 423Lipoyl synthase 1, mitochondrialAdd BLAST423

Proteomic databases

PaxDbiQ4DC43
PRIDEiQ4DC43

Interactioni

Protein-protein interaction databases

STRINGi353153.XP_811939.1

Structurei

3D structure databases

ProteinModelPortaliQ4DC43
SMRiQ4DC43
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2672 Eukaryota
COG0320 LUCA
KOiK03644

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR031691 LIAS_N
IPR003698 Lipoyl_synth
IPR007197 rSAM
PfamiView protein in Pfam
PF16881 LIAS_N, 1 hit
PF04055 Radical_SAM, 1 hit
SFLDiSFLDG01058 lipoyl_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit

Sequencei

Sequence statusi: Complete.

Q4DC43-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFHRHLCKLC SKTPSAATLA SPLGKLQEER GEGVAKDLKK DKQHRQIFLQ
60 70 80 90 100
KFRERLDSDT TGKNTLAGFI DLPEGISPTM AAVGPLKRGE EPLPPWLKMK
110 120 130 140 150
VAKGVSRLPR FNSIRKSMRE KRLATVCEEA KCPNIGECWG GDEEEGTATA
160 170 180 190 200
TIMVMGSHCT RGCRFCSVLT SRTPPPLDPD EPQKVANAVA EMGVDYIVMT
210 220 230 240 250
MVDRDDLNDG GAAHVVRCVN AIKEKNPLLL LEALVGDFHG DLKLVETVAL
260 270 280 290 300
SPLSVYAHNI ECVERITPNV RDRRASYRQS LKVLEHVNSF TKGAMLTKSS
310 320 330 340 350
IMLGLGEKEE EVRQTLRDLR TAGVSAVTLG QYLQPARTRL KVSRYAHPKE
360 370 380 390 400
FQMWEEEAMA MGFLYCASGP LVRSSYRAGE YYIKSLVKQR GAAATKSNTT
410 420
TTTTNTASLA AATVTDSATL QGE
Length:423
Mass (Da):46,531
Last modified:September 13, 2005 - v1
Checksum:i7EBC5CA8279BA97D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAHK01000671 Genomic DNA Translation: EAN90088.1
RefSeqiXP_811939.1, XM_806846.1

Genome annotation databases

EnsemblProtistsiEAN90088; EAN90088; Tc00.1047053511291.30
GeneDBiTcCLB.511291.30:pep
GeneIDi3543008
KEGGitcr:511291.30

Similar proteinsi

Entry informationi

Entry nameiLIPA1_TRYCC
AccessioniPrimary (citable) accession number: Q4DC43
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: September 13, 2005
Last modified: May 23, 2018
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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