Q4DBW3 (JBP1A_TRYCC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymine dioxygenase JBP1-A
EC=1.14.11.6

Alternative name(s):
J-binding protein 1A
Thymidine hydroxylase JBP1-A

Gene names

Name:	JBP1A
ORF Names:	Tc00.1047053510357.10

Organism

Trypanosoma cruzi (strain CL Brener) [Complete proteome]

Taxonomic identifier

353153 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Trypanosoma › Schizotrypanum › Trypanosoma cruzi

Protein attributes

Sequence length	832 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Dioxygenase that catalyzes the first step of DNA base J (beta-d-glucosyl-HOMedU) biosynthesis by converting thymine to 5-hydroxymethyluracil (HOMedU). DNA base J is a hypermodified thymidine residue found in the genome of kinetoplastid parasites, which is localized primarily to repetitive DNA, namely the telomeres, and is implicated in the regulation of antigenic variation. Also specifically binds to base J-containing DNA (J-DNA). Involved in propagation and maintenance of DNA base J synthesis initiated by JBP2 by specifically binding already synthesized DNA base J and propagating J synthesis. Thymine dioxygenase activity and J-DNA-binding are independent functions By similarity.
Catalytic activity	Thymine + 2-oxoglutarate + O2 = 5-hydroxymethyluracil + succinate + CO2.
Cofactor	Binds 1 Fe2+ ion per subunit By similarity.
Subunit structure	Monomer. Binds to DNA as a monomer By similarity.
Subcellular location	Nucleus By similarity.
Domain	The DNA-binding JBP1 domain (DB-JBP1) is necessary and sufficient for binding to J-DNA By similarity.
Sequence similarities	Belongs to the TET family. JBP1 subfamily.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	DNA-binding Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW thymine dioxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 832	832	Thymine dioxygenase JBP1-A		PRO_0000377556
Regions
	Region	80 – 282	203	Thymine dioxygenase By similarity
	Region	409 – 578	170	DNA-binding JBP1 domain By similarity
Sites
	Metal binding	207	1	Iron; catalytic By similarity
	Metal binding	209	1	Iron; catalytic By similarity
	Metal binding	257	1	Iron; catalytic By similarity
	Binding site	273	1	2-oxoglutarate By similarity
	Site	542	1	Involved in J base recognition, conferring specificity towards J-DNA By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4DBW3 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 78C00B8F2ABF54FA	FASTA	832	93,816
	10 20 30 40 50 60 MKQKRGKQDV KMLESAPPQL LPKKGRLEIS ELAPQQRTIR TAEEIETAYN EAVRKHPFYD 70 80 90 100 110 120 NADHTIDFHD ATVFRDARGV VGGVFLPGAL PAFAATMAAD VLRPAAVRTS LRSNMFGGFA 130 140 150 160 170 180 PLSGIAGYFD YRGSPVELKC RKTSFTYENV HSWPNVFPMI DYVSAIYKAV FPEQWAAQDA 190 200 210 220 230 240 AVPDIVRIHG SPFSTLTVNQ QFRTASHTDA GDFDMGYGLL AVLEGKFEGL SLALDDFGVC 250 260 270 280 290 300 FRMQPRDILI FNTHFFHSNT EPELNHPRDD WSRLTCVCYY RAALGEPACV AEYERRLARA 310 320 330 340 350 360 KEIGASPPPA VDAILQKDNG NNFNKPAPTF PYLLTPFGGA ASVCSLHCCT AKLLRLHELL 370 380 390 400 410 420 LENPTLEVIL FGESLRTDDG LPRREKEQLI SVHLPVVVKM SPSGGFSELG GALKAAEEKQ 430 440 450 460 470 480 YFFEEKYLAD ELGPDLMSMW TQSRAHWLRL VKEDWERLCR RDPERTKFTW NNSSAMNAAF 490 500 510 520 530 540 FDLCEVAKQM MIGLLNKETP SSAENHSFWI LFAAHLNYAC TTENGMPRDA VGMHKLNVKL 550 560 570 580 590 600 KDFHFGGTRY LKDMPPEEQE RRLERKKRIE EARRRGSSAH ETHTDNWLLN DTFDYQQEDR 610 620 630 640 650 660 KVEFEENGWM TPEAYVKHLG LKPCGDVTAA ASPTEPIHVL VVLPRPAAAA TAKDAKRDVP 670 680 690 700 710 720 LATSEESIRL LMNPAAQRVL RGKARNVALP SPLSFGGVKI TVLFDGDDID CIHPDFVILQ 730 740 750 760 770 780 HLLATIEEDE AAKARVKYWA RVARYCVFVV ETDVRDRRHF LLREEVRVAY EDVAEDCFRS 790 800 810 820 830 LHAAAYSTKY NRLRTTPSLI ALCNRKNIGL RFKFRGSPLN TIALVVVGER LD

References

[1]

"The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease." El-Sayed N.M.A., Myler P.J., Bartholomeu D.C., Nilsson D., Aggarwal G., Tran A.-N., Ghedin E., Worthey E.A., Delcher A.L., Blandin G., Westenberger S.J., Caler E., Cerqueira G.C., Branche C., Haas B., Anupama A., Arner E., Aslund L. , Attipoe P., Bontempi E., Bringaud F., Burton P., Cadag E., Campbell D.A., Carrington M., Crabtree J., Darban H., da Silveira J.F., de Jong P., Edwards K., Englund P.T., Fazelina G., Feldblyum T., Ferella M., Frasch A.C., Gull K., Horn D., Hou L., Huang Y., Kindlund E., Klingbeil M., Kluge S., Koo H., Lacerda D., Levin M.J., Lorenzi H., Louie T., Machado C.R., McCulloch R., McKenna A., Mizuno Y., Mottram J.C., Nelson S., Ochaya S., Osoegawa K., Pai G., Parsons M., Pentony M., Pettersson U., Pop M., Ramirez J.L., Rinta J., Robertson L., Salzberg S.L., Sanchez D.O., Seyler A., Sharma R., Shetty J., Simpson A.J., Sisk E., Tammi M.T., Tarleton R., Teixeira S., Van Aken S., Vogt C., Ward P.N., Wickstead B., Wortman J., White O., Fraser C.M., Stuart K.D., Andersson B. Science 309:409-415(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CL Brener.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAHK01000683 Genomic DNA. Translation: EAN90000.1.
RefSeq	XP_811851.1. XM_806758.1.
3D structure databases
ProteinModelPortal	Q4DBW3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3542898.
KEGG	tcr:510357.10.
Phylogenomic databases
ProtClustDB	CLSZ2441100.
Family and domain databases
InterPro	IPR024779. 2OGFeDO_nucleic_acid_mod. [Graphical view]
Pfam	PF12851. Tet_JBP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

JBP1A_TRYCC

Accession

Primary (citable) accession number: Q4DBW3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2009
Last sequence update:	September 13, 2005
Last modified:	February 6, 2013
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)